A novel bioreactor with mechanical stimulation for skeletal tissue engineering
Authors
Petrović, Miloš
Mitraković, Dragan
Bugarski, Branko

Vonwil, Daniel
Martin, Ivan

Obradović, Bojana

Article (Published version)
Metadata
Show full item recordAbstract
The provision of mechanical stimulation is believed to be necessary for the functional assembly of skeletal tissues, which are normally exposed to a variety of biomechanical signals in vivo. In this paper, we present a development and validation of a novel bioreactor aimed for skeletal tissue engineering that provides dynamic compression and perfusion of cultivated tissues. Dynamic compression can be applied at frequencies up to 67.5 Hz and displacements down to 5 µm thus suitable for the simulation of physiological conditions in a native cartilage tissue (0.1-1 Hz, 5-10 % strain). The bioreactor also includes a load sensor that was calibrated so to measure average loads imposed on tissue samples. Regimes of the mechanical stimulation and acquisition of load sensor outputs are directed by an automatic control system using applications developed within the LabView platform. In addition, perfusion of tissue samples at physiological velocities (10-100 µm/s) provides efficient mass transfe...r, as well as the possibilities to expose the cells to hydrodynamic shear and simulate the conditions in a native bone tissue. Thus, the novel bioreactor is suited for studies of the effects of different biomechanical signals on in vitro regeneration of skeletal tissues, as well as for the studies of newly formulated biomaterials and cell biomaterial interactions under in vivo-like settings.
Keywords:
bioreactor / dynamic compression / perfusion / cartilage / bone / tissue engineeringSource:
Chemical Industry & Chemical Engineering Quarterly, 2009, 15, 1, 41-44Publisher:
- Association of the Chemical Engineers of Serbia
Funding / projects:
- Swiss National Science FoundationSwiss National Science Foundation (SNSF) [IB73B0-111016/1]
- Interakcija imobilisanih ćelija, tkiva i biološki aktivnih molekula u bioreaktorskim sistemima (RS-142075)
DOI: 10.2298/CICEQ0901041P
ISSN: 1451-9372
WoS: 000263780100011
Scopus: 2-s2.0-74049103091
Institution/Community
Tehnološko-metalurški fakultetTY - JOUR AU - Petrović, Miloš AU - Mitraković, Dragan AU - Bugarski, Branko AU - Vonwil, Daniel AU - Martin, Ivan AU - Obradović, Bojana PY - 2009 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1453 AB - The provision of mechanical stimulation is believed to be necessary for the functional assembly of skeletal tissues, which are normally exposed to a variety of biomechanical signals in vivo. In this paper, we present a development and validation of a novel bioreactor aimed for skeletal tissue engineering that provides dynamic compression and perfusion of cultivated tissues. Dynamic compression can be applied at frequencies up to 67.5 Hz and displacements down to 5 µm thus suitable for the simulation of physiological conditions in a native cartilage tissue (0.1-1 Hz, 5-10 % strain). The bioreactor also includes a load sensor that was calibrated so to measure average loads imposed on tissue samples. Regimes of the mechanical stimulation and acquisition of load sensor outputs are directed by an automatic control system using applications developed within the LabView platform. In addition, perfusion of tissue samples at physiological velocities (10-100 µm/s) provides efficient mass transfer, as well as the possibilities to expose the cells to hydrodynamic shear and simulate the conditions in a native bone tissue. Thus, the novel bioreactor is suited for studies of the effects of different biomechanical signals on in vitro regeneration of skeletal tissues, as well as for the studies of newly formulated biomaterials and cell biomaterial interactions under in vivo-like settings. PB - Association of the Chemical Engineers of Serbia T2 - Chemical Industry & Chemical Engineering Quarterly T1 - A novel bioreactor with mechanical stimulation for skeletal tissue engineering EP - 44 IS - 1 SP - 41 VL - 15 DO - 10.2298/CICEQ0901041P ER -
@article{ author = "Petrović, Miloš and Mitraković, Dragan and Bugarski, Branko and Vonwil, Daniel and Martin, Ivan and Obradović, Bojana", year = "2009", abstract = "The provision of mechanical stimulation is believed to be necessary for the functional assembly of skeletal tissues, which are normally exposed to a variety of biomechanical signals in vivo. In this paper, we present a development and validation of a novel bioreactor aimed for skeletal tissue engineering that provides dynamic compression and perfusion of cultivated tissues. Dynamic compression can be applied at frequencies up to 67.5 Hz and displacements down to 5 µm thus suitable for the simulation of physiological conditions in a native cartilage tissue (0.1-1 Hz, 5-10 % strain). The bioreactor also includes a load sensor that was calibrated so to measure average loads imposed on tissue samples. Regimes of the mechanical stimulation and acquisition of load sensor outputs are directed by an automatic control system using applications developed within the LabView platform. In addition, perfusion of tissue samples at physiological velocities (10-100 µm/s) provides efficient mass transfer, as well as the possibilities to expose the cells to hydrodynamic shear and simulate the conditions in a native bone tissue. Thus, the novel bioreactor is suited for studies of the effects of different biomechanical signals on in vitro regeneration of skeletal tissues, as well as for the studies of newly formulated biomaterials and cell biomaterial interactions under in vivo-like settings.", publisher = "Association of the Chemical Engineers of Serbia", journal = "Chemical Industry & Chemical Engineering Quarterly", title = "A novel bioreactor with mechanical stimulation for skeletal tissue engineering", pages = "44-41", number = "1", volume = "15", doi = "10.2298/CICEQ0901041P" }
Petrović, M., Mitraković, D., Bugarski, B., Vonwil, D., Martin, I.,& Obradović, B.. (2009). A novel bioreactor with mechanical stimulation for skeletal tissue engineering. in Chemical Industry & Chemical Engineering Quarterly Association of the Chemical Engineers of Serbia., 15(1), 41-44. https://doi.org/10.2298/CICEQ0901041P
Petrović M, Mitraković D, Bugarski B, Vonwil D, Martin I, Obradović B. A novel bioreactor with mechanical stimulation for skeletal tissue engineering. in Chemical Industry & Chemical Engineering Quarterly. 2009;15(1):41-44. doi:10.2298/CICEQ0901041P .
Petrović, Miloš, Mitraković, Dragan, Bugarski, Branko, Vonwil, Daniel, Martin, Ivan, Obradović, Bojana, "A novel bioreactor with mechanical stimulation for skeletal tissue engineering" in Chemical Industry & Chemical Engineering Quarterly, 15, no. 1 (2009):41-44, https://doi.org/10.2298/CICEQ0901041P . .