Microwave-assisted immobilization of lipase from Candida rugosa on Eupergit (R) supports
Samo za registrovane korisnike
2009
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
BACKGROUND: Immobilization of lipase (triacylglycerol acylhydrolase EC 3.1.1.3) from Candida rugosa on Eupergite (R) C and Eupergit (R) C 250L was performed under microwave irradiation in order to reduce immobilization time. Lipase loading, hydrolytic activity, esterification activity and operational stability in organic solvent of immobilized lipase preparation were determined. RESULTS: The microwave-assisted procedure resulted in a 29% lower lipase loadings, compared with immobilized lipase obtained without microwaves. In hydrolytic activity assay, lipase immobilized under microwaves exhibited a 23% higher specific activity. Slight activation of lipase by microwave-assisted immobilization was observed, since specific activity was around 5% higher than for free lipase. Lipase of highest activity was obtained after 2 min immobilization on Eupergit (R) C. The same preparation exhibited high esterification activity in organic medium and a half life of 212 h was determined in multiple use... assay. CONCLUSION: The application of microwave irradiation leads to reduction of immobilization time from 2 days to only 2 min. The immobilized lipase obtained has prospects for further application due to its high retained activity and stability.
Ključne reči:
lipase / Eupergit (R) / microwave / immobilization / esterificationIzvor:
Journal of Chemical Technology and Biotechnology, 2009, 84, 11, 1642-1648Izdavač:
- Wiley, Hoboken
Finansiranje / projekti:
- Razvoj biotehnoloških postupaka za proizvodnju aditiva i novih formulacija za prehrambenu industriju (RS-MESTD-MPN2006-2010-20064)
DOI: 10.1002/jctb.2222
ISSN: 0268-2575
WoS: 000271585100005
Scopus: 2-s2.0-70449344233
Institucija/grupa
Tehnološko-metalurški fakultetTY - JOUR AU - Bezbradica, Dejan AU - Mijin, Dušan AU - Mihailović, Mladen AU - Knežević-Jugović, Zorica PY - 2009 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1455 AB - BACKGROUND: Immobilization of lipase (triacylglycerol acylhydrolase EC 3.1.1.3) from Candida rugosa on Eupergite (R) C and Eupergit (R) C 250L was performed under microwave irradiation in order to reduce immobilization time. Lipase loading, hydrolytic activity, esterification activity and operational stability in organic solvent of immobilized lipase preparation were determined. RESULTS: The microwave-assisted procedure resulted in a 29% lower lipase loadings, compared with immobilized lipase obtained without microwaves. In hydrolytic activity assay, lipase immobilized under microwaves exhibited a 23% higher specific activity. Slight activation of lipase by microwave-assisted immobilization was observed, since specific activity was around 5% higher than for free lipase. Lipase of highest activity was obtained after 2 min immobilization on Eupergit (R) C. The same preparation exhibited high esterification activity in organic medium and a half life of 212 h was determined in multiple use assay. CONCLUSION: The application of microwave irradiation leads to reduction of immobilization time from 2 days to only 2 min. The immobilized lipase obtained has prospects for further application due to its high retained activity and stability. PB - Wiley, Hoboken T2 - Journal of Chemical Technology and Biotechnology T1 - Microwave-assisted immobilization of lipase from Candida rugosa on Eupergit (R) supports EP - 1648 IS - 11 SP - 1642 VL - 84 DO - 10.1002/jctb.2222 ER -
@article{ author = "Bezbradica, Dejan and Mijin, Dušan and Mihailović, Mladen and Knežević-Jugović, Zorica", year = "2009", abstract = "BACKGROUND: Immobilization of lipase (triacylglycerol acylhydrolase EC 3.1.1.3) from Candida rugosa on Eupergite (R) C and Eupergit (R) C 250L was performed under microwave irradiation in order to reduce immobilization time. Lipase loading, hydrolytic activity, esterification activity and operational stability in organic solvent of immobilized lipase preparation were determined. RESULTS: The microwave-assisted procedure resulted in a 29% lower lipase loadings, compared with immobilized lipase obtained without microwaves. In hydrolytic activity assay, lipase immobilized under microwaves exhibited a 23% higher specific activity. Slight activation of lipase by microwave-assisted immobilization was observed, since specific activity was around 5% higher than for free lipase. Lipase of highest activity was obtained after 2 min immobilization on Eupergit (R) C. The same preparation exhibited high esterification activity in organic medium and a half life of 212 h was determined in multiple use assay. CONCLUSION: The application of microwave irradiation leads to reduction of immobilization time from 2 days to only 2 min. The immobilized lipase obtained has prospects for further application due to its high retained activity and stability.", publisher = "Wiley, Hoboken", journal = "Journal of Chemical Technology and Biotechnology", title = "Microwave-assisted immobilization of lipase from Candida rugosa on Eupergit (R) supports", pages = "1648-1642", number = "11", volume = "84", doi = "10.1002/jctb.2222" }
Bezbradica, D., Mijin, D., Mihailović, M.,& Knežević-Jugović, Z.. (2009). Microwave-assisted immobilization of lipase from Candida rugosa on Eupergit (R) supports. in Journal of Chemical Technology and Biotechnology Wiley, Hoboken., 84(11), 1642-1648. https://doi.org/10.1002/jctb.2222
Bezbradica D, Mijin D, Mihailović M, Knežević-Jugović Z. Microwave-assisted immobilization of lipase from Candida rugosa on Eupergit (R) supports. in Journal of Chemical Technology and Biotechnology. 2009;84(11):1642-1648. doi:10.1002/jctb.2222 .
Bezbradica, Dejan, Mijin, Dušan, Mihailović, Mladen, Knežević-Jugović, Zorica, "Microwave-assisted immobilization of lipase from Candida rugosa on Eupergit (R) supports" in Journal of Chemical Technology and Biotechnology, 84, no. 11 (2009):1642-1648, https://doi.org/10.1002/jctb.2222 . .