Biocompatible and bioactive nanostructured glass coatings synthesized by pulsed laser deposition: In vitro biological tests
Authorized Users Only
2009
Authors
Popescu, A. C.
Sima, F.

Duta, L.
Popescu, A. C.
Mihailescu, Ion N.
Capitanu, D.
Mustata, R.
Sima, L. E.

Petrescu, S. M.

Janaćković, Đorđe

Article (Published version)

Metadata
Show full item recordAbstract
We report on the synthesis by pulsed laser deposition with a KrF* excimer laser source (lambda = 248 nm, tau = 25 ns) of bioglass thin films of 6P57 and 6P61 types. Physiology, viability, and proliferation of human osteoblast cells were determined by quantitative in vitro tests performed by flow cytometry on primary osteoblasts cultured on pulsed laser deposited bioglasses. Both types of glass films proved to be appropriate mediums for cell survival and proliferation. In a parallel investigation, cell morphology and adhesion to the surface was studied by fluorescence microscopy and scanning electron microscopy. Strong bonds between the materials and cells were found in both cases, as osteoblast pseudopodes penetrated deep into the material. According to our observations, the 6P57 glass films were superior with respect to viability and proliferation performances.
Keywords:
Bioglass thin films / In vitro biotests / Pulsed laser depositionSource:
Applied Surface Science, 2009, 255, 10, 5486-5490Publisher:
- Elsevier, Amsterdam
DOI: 10.1016/j.apsusc.2008.10.031
ISSN: 0169-4332
WoS: 000263865000085
Scopus: 2-s2.0-61749095129
Institution/Community
Tehnološko-metalurški fakultetTY - JOUR AU - Popescu, A. C. AU - Sima, F. AU - Duta, L. AU - Popescu, A. C. AU - Mihailescu, Ion N. AU - Capitanu, D. AU - Mustata, R. AU - Sima, L. E. AU - Petrescu, S. M. AU - Janaćković, Đorđe PY - 2009 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1483 AB - We report on the synthesis by pulsed laser deposition with a KrF* excimer laser source (lambda = 248 nm, tau = 25 ns) of bioglass thin films of 6P57 and 6P61 types. Physiology, viability, and proliferation of human osteoblast cells were determined by quantitative in vitro tests performed by flow cytometry on primary osteoblasts cultured on pulsed laser deposited bioglasses. Both types of glass films proved to be appropriate mediums for cell survival and proliferation. In a parallel investigation, cell morphology and adhesion to the surface was studied by fluorescence microscopy and scanning electron microscopy. Strong bonds between the materials and cells were found in both cases, as osteoblast pseudopodes penetrated deep into the material. According to our observations, the 6P57 glass films were superior with respect to viability and proliferation performances. PB - Elsevier, Amsterdam T2 - Applied Surface Science T1 - Biocompatible and bioactive nanostructured glass coatings synthesized by pulsed laser deposition: In vitro biological tests EP - 5490 IS - 10 SP - 5486 VL - 255 DO - 10.1016/j.apsusc.2008.10.031 ER -
@article{ author = "Popescu, A. C. and Sima, F. and Duta, L. and Popescu, A. C. and Mihailescu, Ion N. and Capitanu, D. and Mustata, R. and Sima, L. E. and Petrescu, S. M. and Janaćković, Đorđe", year = "2009", abstract = "We report on the synthesis by pulsed laser deposition with a KrF* excimer laser source (lambda = 248 nm, tau = 25 ns) of bioglass thin films of 6P57 and 6P61 types. Physiology, viability, and proliferation of human osteoblast cells were determined by quantitative in vitro tests performed by flow cytometry on primary osteoblasts cultured on pulsed laser deposited bioglasses. Both types of glass films proved to be appropriate mediums for cell survival and proliferation. In a parallel investigation, cell morphology and adhesion to the surface was studied by fluorescence microscopy and scanning electron microscopy. Strong bonds between the materials and cells were found in both cases, as osteoblast pseudopodes penetrated deep into the material. According to our observations, the 6P57 glass films were superior with respect to viability and proliferation performances.", publisher = "Elsevier, Amsterdam", journal = "Applied Surface Science", title = "Biocompatible and bioactive nanostructured glass coatings synthesized by pulsed laser deposition: In vitro biological tests", pages = "5490-5486", number = "10", volume = "255", doi = "10.1016/j.apsusc.2008.10.031" }
Popescu, A. C., Sima, F., Duta, L., Popescu, A. C., Mihailescu, I. N., Capitanu, D., Mustata, R., Sima, L. E., Petrescu, S. M.,& Janaćković, Đ.. (2009). Biocompatible and bioactive nanostructured glass coatings synthesized by pulsed laser deposition: In vitro biological tests. in Applied Surface Science Elsevier, Amsterdam., 255(10), 5486-5490. https://doi.org/10.1016/j.apsusc.2008.10.031
Popescu AC, Sima F, Duta L, Popescu AC, Mihailescu IN, Capitanu D, Mustata R, Sima LE, Petrescu SM, Janaćković Đ. Biocompatible and bioactive nanostructured glass coatings synthesized by pulsed laser deposition: In vitro biological tests. in Applied Surface Science. 2009;255(10):5486-5490. doi:10.1016/j.apsusc.2008.10.031 .
Popescu, A. C., Sima, F., Duta, L., Popescu, A. C., Mihailescu, Ion N., Capitanu, D., Mustata, R., Sima, L. E., Petrescu, S. M., Janaćković, Đorđe, "Biocompatible and bioactive nanostructured glass coatings synthesized by pulsed laser deposition: In vitro biological tests" in Applied Surface Science, 255, no. 10 (2009):5486-5490, https://doi.org/10.1016/j.apsusc.2008.10.031 . .