Show simple item record

dc.creatorKovatcheva-Datchary, Petia
dc.creatorEgert, Markus
dc.creatorMaathuis, Annet
dc.creatorRajilić-Stojanović, Mirjana
dc.creatorde Graaf, Albert A.
dc.creatorSmidt, Hauke
dc.creatorde Vos, Willem M.
dc.creatorVenema, Koen
dc.date.accessioned2021-03-10T11:09:07Z
dc.date.available2021-03-10T11:09:07Z
dc.date.issued2009
dc.identifier.issn1462-2912
dc.identifier.urihttp://TechnoRep.tmf.bg.ac.rs/handle/123456789/1487
dc.description.abstractCarbohydrates, including starches, are an important energy source for humans, and are known for their interactions with the microbiota in the digestive tract. Largely, those interactions are thought to promote human health. Using 16S ribosomal RNA (rRNA)-based stable isotope probing (SIP), we identified starch-fermenting bacteria under human colon-like conditions. To the microbiota of the TIM-2 in vitro model of the human colon 7.4 g l(-1) of [U-(13)C]-starch was added. RNA extracted from lumen samples after 0 (control), 2, 4 and 8 h was subjected to density-gradient ultracentrifugation. Terminal-restriction fragment length polymorphism (T-RFLP) fingerprinting and phylogenetic analyses of the labelled and unlabelled 16S rRNA suggested populations related to Ruminococcus bromii, Prevotella spp. and Eubacterium rectale to be involved in starch metabolism. Additionally, 16S rRNA related to that of Bifidobacterium adolescentis was abundant in all analysed fractions. While this might be due to the enrichment of high-GC RNA in high-density fractions, it could also indicate an active role in starch fermentation. Comparison of the T-RFLP fingerprints of experiments performed with labelled and unlabelled starch revealed Ruminococcus bromii as the primary degrader in starch fermentation in the studied model, as it was found to solely predominate in the labelled fractions. LC-MS analyses of the lumen and dialysate samples showed that, for both experiments, starch fermentation primarily yielded acetate, butyrate and propionate. Integration of molecular and metabolite data suggests metabolic cross-feeding in the system, where populations related to Ruminococcus bromii are the primary starch degrader, while those related to Prevotella spp., Bifidobacterium adolescentis and Eubacterium rectale might be further involved in the trophic chain.en
dc.publisherWiley-Blackwell, Malden
dc.rightsrestrictedAccess
dc.sourceEnvironmental Microbiology
dc.titleLinking phylogenetic identities of bacteria to starch fermentation in an in vitro model of the large intestine by RNA-based stable isotope probingen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage926
dc.citation.issue4
dc.citation.other11(4): 914-926
dc.citation.rankM21
dc.citation.spage914
dc.citation.volume11
dc.identifier.doi10.1111/j.1462-2920.2008.01815.x
dc.identifier.pmid19128319
dc.identifier.rcubconv_3161
dc.identifier.scopus2-s2.0-63849262393
dc.identifier.wos000264881300017
dc.type.versionpublishedVersion


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record