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dc.creatorLu, Xiyang
dc.creatorYao, Yao
dc.creatorYang, Yang
dc.creatorZhang, Zhongxi
dc.creatorGu, Jinjie
dc.creatorMojović, Ljiljana
dc.creatorKnežević-Jugović, Zorica
dc.creatorBaganz, Frank
dc.creatorLye, Gary
dc.creatorShi, Jiping
dc.creatorHao, Jian
dc.date.accessioned2021-03-10T09:33:16Z
dc.date.available2021-03-10T09:33:16Z
dc.date.issued2021
dc.identifier.issn0885-4513
dc.identifier.urihttp://TechnoRep.tmf.bg.ac.rs/handle/123456789/14
dc.description.abstractEthylene glycol and glycolic acid are bulk chemicals with a broad range of applications. The ethylene glycol and glycolic acid biosynthesis pathways have been produced by microorganisms and used as a biological route for their production. Unlike the methods that use xylose or glucose as carbon sources, xylonic acid was used as a carbon source to produce ethylene glycol and glycolic acid in this study. Amounts of 4.2 g/L of ethylene glycol and 0.7 g/L of glycolic acid were produced by a wild-typeEscherichia coliW3110 within 10 H of cultivation with a substrate conversion ratio of 0.5 mol/mol. Furthermore,E. colistrains that produce solely ethylene glycol or glycolic acid were constructed. 10.3 g/L of glycolic acid was produced byE. coli Delta yqhD+aldA, and the achieved conversion ratio was 0.56 mol/mol. Similarly, theE. coli Delta aldA+yqhDproduced 8.0 g/L of ethylene glycol with a conversion ratio of 0.71 mol/mol. Ethylene glycol and glycolic acid production byE. colion xylonic acid as a carbon source provides new information on the biosynthesis pathway of these products and opens a novel way of biomass utilization.en
dc.publisherWiley, Hoboken
dc.relationNational Key R&D Program of China [2017YFE0112700]
dc.relationRoyal Society-Newton Advanced Fellowship
dc.relationNAF [\R2\180721]
dc.relationNatural Science Foundation of ShanghaiNatural Science Foundation of Shanghai [19ZR1463600]
dc.relationNational Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [21576279]
dc.rightsrestrictedAccess
dc.sourceBiotechnology and Applied Biochemistry
dc.subjectEen
dc.subjectcolien
dc.subjectethylene glycolen
dc.subjectglycolic aciden
dc.subjectxylonic aciden
dc.subjectxyloseen
dc.titleEthylene glycol and glycolic acid production by wild-typeEscherichia colien
dc.typearticle
dc.rights.licenseARR
dc.citation.epage755
dc.citation.issue4
dc.citation.rankM23
dc.citation.spage744
dc.citation.spage755
dc.citation.volume68
dc.identifier.doi10.1002/bab.1987
dc.identifier.pmid32683722
dc.identifier.scopus2-s2.0-85088944961
dc.identifier.wos000553676700001
dc.type.versionpublishedVersion


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Приказ основних података о документу