Interaction of cells with polymer networks in nanostructured devices as alginate beads for cell immobilization are considered. Influence of process parameters as oxygen concentration inside a bead on changes of alginate material structure is experimentally investigated. Significant swelling of alginate beads during process is observed and ascribed to combined action of chemical components in the reactor and of growing cells, causing expansion of beads and degradation of their networks. The culture of mouse/mouse hybridoma cells secreting a monoclonal antibody (IgG) specific to human chorionic gonadotropin (beta-hCG) was used. A kinetic model of catalytic alginate degradation is developed for controlling the process. The model provides possibilities to analyze conditions for suppressing large swelling and influence of oxidative agents. Moreover, it provides some deeper insights into mechanism of polymer network-cell interactions and dynamics during the process. It is based on separation... of contributions of reactants in the system from contributions of cells to degradation of the network and in that way to effective swelling of the bead.
TY - JOUR
AU - Plavšić, Milenko B.
AU - Pajić-Lijaković, Ivana
AU - Lazić, N.
AU - Bugarski, Branko
AU - Putanov, P.
PY - 2009
UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1528
AB - Interaction of cells with polymer networks in nanostructured devices as alginate beads for cell immobilization are considered. Influence of process parameters as oxygen concentration inside a bead on changes of alginate material structure is experimentally investigated. Significant swelling of alginate beads during process is observed and ascribed to combined action of chemical components in the reactor and of growing cells, causing expansion of beads and degradation of their networks. The culture of mouse/mouse hybridoma cells secreting a monoclonal antibody (IgG) specific to human chorionic gonadotropin (beta-hCG) was used. A kinetic model of catalytic alginate degradation is developed for controlling the process. The model provides possibilities to analyze conditions for suppressing large swelling and influence of oxidative agents. Moreover, it provides some deeper insights into mechanism of polymer network-cell interactions and dynamics during the process. It is based on separation of contributions of reactants in the system from contributions of cells to degradation of the network and in that way to effective swelling of the bead.
PB - Taylor & Francis Inc, Philadelphia
T2 - Materials and Manufacturing Processes
T1 - Catalytic Degradation Processes and Swelling of Alginate Bio-Medical Gels under Influence of Oxygen
EP - 1196
IS - 10-11
SP - 1190
VL - 24
DO - 10.1080/10426910903031727
ER -
@article{
author = "Plavšić, Milenko B. and Pajić-Lijaković, Ivana and Lazić, N. and Bugarski, Branko and Putanov, P.",
year = "2009",
abstract = "Interaction of cells with polymer networks in nanostructured devices as alginate beads for cell immobilization are considered. Influence of process parameters as oxygen concentration inside a bead on changes of alginate material structure is experimentally investigated. Significant swelling of alginate beads during process is observed and ascribed to combined action of chemical components in the reactor and of growing cells, causing expansion of beads and degradation of their networks. The culture of mouse/mouse hybridoma cells secreting a monoclonal antibody (IgG) specific to human chorionic gonadotropin (beta-hCG) was used. A kinetic model of catalytic alginate degradation is developed for controlling the process. The model provides possibilities to analyze conditions for suppressing large swelling and influence of oxidative agents. Moreover, it provides some deeper insights into mechanism of polymer network-cell interactions and dynamics during the process. It is based on separation of contributions of reactants in the system from contributions of cells to degradation of the network and in that way to effective swelling of the bead.",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "Materials and Manufacturing Processes",
title = "Catalytic Degradation Processes and Swelling of Alginate Bio-Medical Gels under Influence of Oxygen",
pages = "1196-1190",
number = "10-11",
volume = "24",
doi = "10.1080/10426910903031727"
}
Plavšić, M. B., Pajić-Lijaković, I., Lazić, N., Bugarski, B.,& Putanov, P.. (2009). Catalytic Degradation Processes and Swelling of Alginate Bio-Medical Gels under Influence of Oxygen. in Materials and Manufacturing Processes
Taylor & Francis Inc, Philadelphia., 24(10-11), 1190-1196.
https://doi.org/10.1080/10426910903031727
Plavšić MB, Pajić-Lijaković I, Lazić N, Bugarski B, Putanov P. Catalytic Degradation Processes and Swelling of Alginate Bio-Medical Gels under Influence of Oxygen. in Materials and Manufacturing Processes. 2009;24(10-11):1190-1196.
doi:10.1080/10426910903031727 .
Plavšić, Milenko B., Pajić-Lijaković, Ivana, Lazić, N., Bugarski, Branko, Putanov, P., "Catalytic Degradation Processes and Swelling of Alginate Bio-Medical Gels under Influence of Oxygen" in Materials and Manufacturing Processes, 24, no. 10-11 (2009):1190-1196,
https://doi.org/10.1080/10426910903031727 . .
