Catalytic Degradation Processes and Swelling of Alginate Bio-Medical Gels under Influence of Oxygen
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2009
Članak u časopisu (Objavljena verzija)

Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Interaction of cells with polymer networks in nanostructured devices as alginate beads for cell immobilization are considered. Influence of process parameters as oxygen concentration inside a bead on changes of alginate material structure is experimentally investigated. Significant swelling of alginate beads during process is observed and ascribed to combined action of chemical components in the reactor and of growing cells, causing expansion of beads and degradation of their networks. The culture of mouse/mouse hybridoma cells secreting a monoclonal antibody (IgG) specific to human chorionic gonadotropin (beta-hCG) was used. A kinetic model of catalytic alginate degradation is developed for controlling the process. The model provides possibilities to analyze conditions for suppressing large swelling and influence of oxidative agents. Moreover, it provides some deeper insights into mechanism of polymer network-cell interactions and dynamics during the process. It is based on separation... of contributions of reactants in the system from contributions of cells to degradation of the network and in that way to effective swelling of the bead.
Ključne reči:
Ageing / Alginates / Free radicals / Hybridoma cells / Immobilized cell lifespan / Immunoglobulin production / Polymer networks / SwellingIzvor:
Materials and Manufacturing Processes, 2009, 24, 10-11, 1190-1196Izdavač:
- Taylor & Francis Inc, Philadelphia
DOI: 10.1080/10426910903031727
ISSN: 1042-6914
WoS: 000274699700026
Scopus: 2-s2.0-77949548262
Institucija/grupa
Tehnološko-metalurški fakultetTY - JOUR AU - Plavšić, Milenko B. AU - Pajić-Lijaković, Ivana AU - Lazić, N. AU - Bugarski, Branko AU - Putanov, P. PY - 2009 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1528 AB - Interaction of cells with polymer networks in nanostructured devices as alginate beads for cell immobilization are considered. Influence of process parameters as oxygen concentration inside a bead on changes of alginate material structure is experimentally investigated. Significant swelling of alginate beads during process is observed and ascribed to combined action of chemical components in the reactor and of growing cells, causing expansion of beads and degradation of their networks. The culture of mouse/mouse hybridoma cells secreting a monoclonal antibody (IgG) specific to human chorionic gonadotropin (beta-hCG) was used. A kinetic model of catalytic alginate degradation is developed for controlling the process. The model provides possibilities to analyze conditions for suppressing large swelling and influence of oxidative agents. Moreover, it provides some deeper insights into mechanism of polymer network-cell interactions and dynamics during the process. It is based on separation of contributions of reactants in the system from contributions of cells to degradation of the network and in that way to effective swelling of the bead. PB - Taylor & Francis Inc, Philadelphia T2 - Materials and Manufacturing Processes T1 - Catalytic Degradation Processes and Swelling of Alginate Bio-Medical Gels under Influence of Oxygen EP - 1196 IS - 10-11 SP - 1190 VL - 24 DO - 10.1080/10426910903031727 ER -
@article{ author = "Plavšić, Milenko B. and Pajić-Lijaković, Ivana and Lazić, N. and Bugarski, Branko and Putanov, P.", year = "2009", abstract = "Interaction of cells with polymer networks in nanostructured devices as alginate beads for cell immobilization are considered. Influence of process parameters as oxygen concentration inside a bead on changes of alginate material structure is experimentally investigated. Significant swelling of alginate beads during process is observed and ascribed to combined action of chemical components in the reactor and of growing cells, causing expansion of beads and degradation of their networks. The culture of mouse/mouse hybridoma cells secreting a monoclonal antibody (IgG) specific to human chorionic gonadotropin (beta-hCG) was used. A kinetic model of catalytic alginate degradation is developed for controlling the process. The model provides possibilities to analyze conditions for suppressing large swelling and influence of oxidative agents. Moreover, it provides some deeper insights into mechanism of polymer network-cell interactions and dynamics during the process. It is based on separation of contributions of reactants in the system from contributions of cells to degradation of the network and in that way to effective swelling of the bead.", publisher = "Taylor & Francis Inc, Philadelphia", journal = "Materials and Manufacturing Processes", title = "Catalytic Degradation Processes and Swelling of Alginate Bio-Medical Gels under Influence of Oxygen", pages = "1196-1190", number = "10-11", volume = "24", doi = "10.1080/10426910903031727" }
Plavšić, M. B., Pajić-Lijaković, I., Lazić, N., Bugarski, B.,& Putanov, P.. (2009). Catalytic Degradation Processes and Swelling of Alginate Bio-Medical Gels under Influence of Oxygen. in Materials and Manufacturing Processes Taylor & Francis Inc, Philadelphia., 24(10-11), 1190-1196. https://doi.org/10.1080/10426910903031727
Plavšić MB, Pajić-Lijaković I, Lazić N, Bugarski B, Putanov P. Catalytic Degradation Processes and Swelling of Alginate Bio-Medical Gels under Influence of Oxygen. in Materials and Manufacturing Processes. 2009;24(10-11):1190-1196. doi:10.1080/10426910903031727 .
Plavšić, Milenko B., Pajić-Lijaković, Ivana, Lazić, N., Bugarski, Branko, Putanov, P., "Catalytic Degradation Processes and Swelling of Alginate Bio-Medical Gels under Influence of Oxygen" in Materials and Manufacturing Processes, 24, no. 10-11 (2009):1190-1196, https://doi.org/10.1080/10426910903031727 . .