Electrochemical determination of glucose using polyaniline electrode modified by glucose oxidize
2010
Authors
Gvozdenović, MilicaJugović, Branimir
Bezbradica, Dejan
Antov, Mirjana
Knežević-Jugović, Zorica
Grgur, Branimir
Conference object (Published version)
Metadata
Show full item recordAbstract
Polyaniline (PANI) enzyme electrode was formed by immobilization of Glucose oxidase (GOx) via glutaraldehyde into electrochemicaly polymerized PANI on graphite electrode. Electrochemical polymerization of PANI on graphite was performed from aqueous solution of 1.0 mol dm-3 HCl and 0.25 mol dm-3 aniline at constant current density of 2.0 mA cm-2. Hronopotentiometric curves of the PANI enzyme electrode obtained at current density of 10 μA cm-2 were recorded in different glucose concentrations. The linearity response range was between 1.0-5.0 mmol dm-3 of glucose concentration. The estimated apparent Michaelis-Menten constant, was Km ′ = 0.30 mmol dm-3, which is scientifically lower than that of free enzyme.
Keywords:
glucose oxidase / polyaniline / immobilization / electrochemical synthesisSource:
The 61st Annual Meeting of the International Society of Electrochemistry: Electrochemistry from Biol, 2010, s11-P-041Publisher:
- Nice : International Society of Electrochemistry
Funding / projects:
- Modifikacija metalnih i nemetalnih materijala elektroprovodnim polimerima za primenu u novim tehnologijama (RS-MESTD-MPN2006-2010-142044)
Institution/Community
Tehnološko-metalurški fakultetTY - CONF AU - Gvozdenović, Milica AU - Jugović, Branimir AU - Bezbradica, Dejan AU - Antov, Mirjana AU - Knežević-Jugović, Zorica AU - Grgur, Branimir PY - 2010 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1575 AB - Polyaniline (PANI) enzyme electrode was formed by immobilization of Glucose oxidase (GOx) via glutaraldehyde into electrochemicaly polymerized PANI on graphite electrode. Electrochemical polymerization of PANI on graphite was performed from aqueous solution of 1.0 mol dm-3 HCl and 0.25 mol dm-3 aniline at constant current density of 2.0 mA cm-2. Hronopotentiometric curves of the PANI enzyme electrode obtained at current density of 10 μA cm-2 were recorded in different glucose concentrations. The linearity response range was between 1.0-5.0 mmol dm-3 of glucose concentration. The estimated apparent Michaelis-Menten constant, was Km ′ = 0.30 mmol dm-3, which is scientifically lower than that of free enzyme. PB - Nice : International Society of Electrochemistry C3 - The 61st Annual Meeting of the International Society of Electrochemistry: Electrochemistry from Biol T1 - Electrochemical determination of glucose using polyaniline electrode modified by glucose oxidize VL - s11-P-041 UR - https://hdl.handle.net/21.15107/rcub_technorep_1575 ER -
@conference{ author = "Gvozdenović, Milica and Jugović, Branimir and Bezbradica, Dejan and Antov, Mirjana and Knežević-Jugović, Zorica and Grgur, Branimir", year = "2010", abstract = "Polyaniline (PANI) enzyme electrode was formed by immobilization of Glucose oxidase (GOx) via glutaraldehyde into electrochemicaly polymerized PANI on graphite electrode. Electrochemical polymerization of PANI on graphite was performed from aqueous solution of 1.0 mol dm-3 HCl and 0.25 mol dm-3 aniline at constant current density of 2.0 mA cm-2. Hronopotentiometric curves of the PANI enzyme electrode obtained at current density of 10 μA cm-2 were recorded in different glucose concentrations. The linearity response range was between 1.0-5.0 mmol dm-3 of glucose concentration. The estimated apparent Michaelis-Menten constant, was Km ′ = 0.30 mmol dm-3, which is scientifically lower than that of free enzyme.", publisher = "Nice : International Society of Electrochemistry", journal = "The 61st Annual Meeting of the International Society of Electrochemistry: Electrochemistry from Biol", title = "Electrochemical determination of glucose using polyaniline electrode modified by glucose oxidize", volume = "s11-P-041", url = "https://hdl.handle.net/21.15107/rcub_technorep_1575" }
Gvozdenović, M., Jugović, B., Bezbradica, D., Antov, M., Knežević-Jugović, Z.,& Grgur, B.. (2010). Electrochemical determination of glucose using polyaniline electrode modified by glucose oxidize. in The 61st Annual Meeting of the International Society of Electrochemistry: Electrochemistry from Biol Nice : International Society of Electrochemistry., s11-P-041. https://hdl.handle.net/21.15107/rcub_technorep_1575
Gvozdenović M, Jugović B, Bezbradica D, Antov M, Knežević-Jugović Z, Grgur B. Electrochemical determination of glucose using polyaniline electrode modified by glucose oxidize. in The 61st Annual Meeting of the International Society of Electrochemistry: Electrochemistry from Biol. 2010;s11-P-041. https://hdl.handle.net/21.15107/rcub_technorep_1575 .
Gvozdenović, Milica, Jugović, Branimir, Bezbradica, Dejan, Antov, Mirjana, Knežević-Jugović, Zorica, Grgur, Branimir, "Electrochemical determination of glucose using polyaniline electrode modified by glucose oxidize" in The 61st Annual Meeting of the International Society of Electrochemistry: Electrochemistry from Biol, s11-P-041 (2010), https://hdl.handle.net/21.15107/rcub_technorep_1575 .