Temperature- and pH-sensitive hydrogels based on N-isopropylacrylamide (NiPAAm) and itaconic acid (IA) were applied for immobilization of lipase from Candida rugosa (CRL). The hydrogels were synthesized by free radical crosslinking copolymerization in the presence of lipase. Characterization of samples by swelling studies, at pH 2.20 and 6.80 at a temperature of 37 degrees C, scanning electron microscopy (SEM) and Fourier transform infrared analysis (FT-IR) confirmed that the degree of crosslinking, the nonionic/ionic (NiPAAm/IA) ratio and the enzyme content had impacts on the hydrogel structure, mechanical properties, morphology and swelling kinetics. All hydrogels demonstrated protein loading efficiencies as high as 95 wt.%. A specific activity of the immobilized lipase of around 38 IU/g was attained for an enzyme loading of 20.0 wt.%. As a result, improved pH and temperature optima values were obtained for the immobilized systems in relation to those for the free lipase.
TY - JOUR
AU - Milašinović, Nikola
AU - Milosavljević, Nedeljko
AU - Filipović, Jovanka
AU - Knežević-Jugović, Zorica
AU - Kalagasidis Krušić, Melina
PY - 2010
UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1586
AB - Temperature- and pH-sensitive hydrogels based on N-isopropylacrylamide (NiPAAm) and itaconic acid (IA) were applied for immobilization of lipase from Candida rugosa (CRL). The hydrogels were synthesized by free radical crosslinking copolymerization in the presence of lipase. Characterization of samples by swelling studies, at pH 2.20 and 6.80 at a temperature of 37 degrees C, scanning electron microscopy (SEM) and Fourier transform infrared analysis (FT-IR) confirmed that the degree of crosslinking, the nonionic/ionic (NiPAAm/IA) ratio and the enzyme content had impacts on the hydrogel structure, mechanical properties, morphology and swelling kinetics. All hydrogels demonstrated protein loading efficiencies as high as 95 wt.%. A specific activity of the immobilized lipase of around 38 IU/g was attained for an enzyme loading of 20.0 wt.%. As a result, improved pH and temperature optima values were obtained for the immobilized systems in relation to those for the free lipase.
PB - Elsevier Science Bv, Amsterdam
T2 - Reactive & Functional Polymers
T1 - Synthesis, characterization and application of poly(N-isopropylacrylamide-co-itaconic acid) hydrogels as supports for lipase immobilization
EP - 814
IS - 10
SP - 807
VL - 70
DO - 10.1016/j.reactfunctpolym.2010.07.017
ER -
@article{
author = "Milašinović, Nikola and Milosavljević, Nedeljko and Filipović, Jovanka and Knežević-Jugović, Zorica and Kalagasidis Krušić, Melina",
year = "2010",
abstract = "Temperature- and pH-sensitive hydrogels based on N-isopropylacrylamide (NiPAAm) and itaconic acid (IA) were applied for immobilization of lipase from Candida rugosa (CRL). The hydrogels were synthesized by free radical crosslinking copolymerization in the presence of lipase. Characterization of samples by swelling studies, at pH 2.20 and 6.80 at a temperature of 37 degrees C, scanning electron microscopy (SEM) and Fourier transform infrared analysis (FT-IR) confirmed that the degree of crosslinking, the nonionic/ionic (NiPAAm/IA) ratio and the enzyme content had impacts on the hydrogel structure, mechanical properties, morphology and swelling kinetics. All hydrogels demonstrated protein loading efficiencies as high as 95 wt.%. A specific activity of the immobilized lipase of around 38 IU/g was attained for an enzyme loading of 20.0 wt.%. As a result, improved pH and temperature optima values were obtained for the immobilized systems in relation to those for the free lipase.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Reactive & Functional Polymers",
title = "Synthesis, characterization and application of poly(N-isopropylacrylamide-co-itaconic acid) hydrogels as supports for lipase immobilization",
pages = "814-807",
number = "10",
volume = "70",
doi = "10.1016/j.reactfunctpolym.2010.07.017"
}
Milašinović, N., Milosavljević, N., Filipović, J., Knežević-Jugović, Z.,& Kalagasidis Krušić, M.. (2010). Synthesis, characterization and application of poly(N-isopropylacrylamide-co-itaconic acid) hydrogels as supports for lipase immobilization. in Reactive & Functional Polymers
Elsevier Science Bv, Amsterdam., 70(10), 807-814.
https://doi.org/10.1016/j.reactfunctpolym.2010.07.017
Milašinović N, Milosavljević N, Filipović J, Knežević-Jugović Z, Kalagasidis Krušić M. Synthesis, characterization and application of poly(N-isopropylacrylamide-co-itaconic acid) hydrogels as supports for lipase immobilization. in Reactive & Functional Polymers. 2010;70(10):807-814.
doi:10.1016/j.reactfunctpolym.2010.07.017 .
Milašinović, Nikola, Milosavljević, Nedeljko, Filipović, Jovanka, Knežević-Jugović, Zorica, Kalagasidis Krušić, Melina, "Synthesis, characterization and application of poly(N-isopropylacrylamide-co-itaconic acid) hydrogels as supports for lipase immobilization" in Reactive & Functional Polymers, 70, no. 10 (2010):807-814,
https://doi.org/10.1016/j.reactfunctpolym.2010.07.017 . .
