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dc.creatorMilašinović, Nikola
dc.creatorMilosavljević, Nedeljko
dc.creatorFilipović, Jovanka
dc.creatorKnežević-Jugović, Zorica
dc.creatorKalagasidis-Krušić, Melina
dc.date.accessioned2021-03-10T11:15:31Z
dc.date.available2021-03-10T11:15:31Z
dc.date.issued2010
dc.identifier.issn1381-5148
dc.identifier.urihttp://TechnoRep.tmf.bg.ac.rs/handle/123456789/1586
dc.description.abstractTemperature- and pH-sensitive hydrogels based on N-isopropylacrylamide (NiPAAm) and itaconic acid (IA) were applied for immobilization of lipase from Candida rugosa (CRL). The hydrogels were synthesized by free radical crosslinking copolymerization in the presence of lipase. Characterization of samples by swelling studies, at pH 2.20 and 6.80 at a temperature of 37 degrees C, scanning electron microscopy (SEM) and Fourier transform infrared analysis (FT-IR) confirmed that the degree of crosslinking, the nonionic/ionic (NiPAAm/IA) ratio and the enzyme content had impacts on the hydrogel structure, mechanical properties, morphology and swelling kinetics. All hydrogels demonstrated protein loading efficiencies as high as 95 wt.%. A specific activity of the immobilized lipase of around 38 IU/g was attained for an enzyme loading of 20.0 wt.%. As a result, improved pH and temperature optima values were obtained for the immobilized systems in relation to those for the free lipase.en
dc.publisherElsevier Science Bv, Amsterdam
dc.relationinfo:eu-repo/grantAgreement/MESTD/MPN2006-2010/142023/RS//
dc.rightsrestrictedAccess
dc.sourceReactive & Functional Polymers
dc.subjectN-Isopropylacrylamideen
dc.subjectItaconic aciden
dc.subjectEnzyme immobilizationen
dc.subjectCandida rugosa lipaseen
dc.subjectEnzyme activityen
dc.titleSynthesis, characterization and application of poly(N-isopropylacrylamide-co-itaconic acid) hydrogels as supports for lipase immobilizationen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage814
dc.citation.issue10
dc.citation.other70(10): 807-814
dc.citation.rankM21
dc.citation.spage807
dc.citation.volume70
dc.identifier.doi10.1016/j.reactfunctpolym.2010.07.017
dc.identifier.rcubconv_3461
dc.identifier.scopus2-s2.0-77956205453
dc.identifier.wos000282460700014
dc.type.versionpublishedVersion


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