Myeloperoxidase-mediated oxidation of organophosphorus pesticides as a pre-step in their determination by AChE based bioanalytical methods
Само за регистроване кориснике
2010
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
In order to improve the sensitivity of assays for inhibitors of the enzyme acetylcholine esterase (AChE), an effective method was developed for the conversion of the organophosphate pesticides (OPs) diazinon, malathion, chlorpyrifos, azinphos-methyl and phorate into more toxic inhibitors. This was accomplished by converting them from the thio form into their oxo form using the enzyme myeloperoxidase. The oxo forms, which are the only products of conversion, were determined by AChE bioassays, using either the free enzyme, or a flow injection analysis manifold with immobilized AChE and spectrophotometric detection. All modified OPs exhibited inhibitory power at ppb levels and within 10 min. The method is considered to represent an excellent means for improving the sensitivity of assays for determination of OPs.
Кључне речи:
Organophosphate pesticides / Myeloperoxidase / Oxidation / Bioanalytics / AcethylcholinesteraseИзвор:
Microchimica Acta, 2010, 170, 3-4, 289-297Издавач:
- Springer Wien, Wien
Финансирање / пројекти:
- Ministry of Science and Environmental Protection of the Republic of Serbia
DOI: 10.1007/s00604-010-0324-2
ISSN: 0026-3672
WoS: 000281384000011
Scopus: 2-s2.0-77956180737
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Lazarević-Pašti, Tamara AU - Momić, Tatjana AU - Onjia, Antonije AU - Vujisić, Ljubodrag V. AU - Vasić, Vesna M. PY - 2010 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1639 AB - In order to improve the sensitivity of assays for inhibitors of the enzyme acetylcholine esterase (AChE), an effective method was developed for the conversion of the organophosphate pesticides (OPs) diazinon, malathion, chlorpyrifos, azinphos-methyl and phorate into more toxic inhibitors. This was accomplished by converting them from the thio form into their oxo form using the enzyme myeloperoxidase. The oxo forms, which are the only products of conversion, were determined by AChE bioassays, using either the free enzyme, or a flow injection analysis manifold with immobilized AChE and spectrophotometric detection. All modified OPs exhibited inhibitory power at ppb levels and within 10 min. The method is considered to represent an excellent means for improving the sensitivity of assays for determination of OPs. PB - Springer Wien, Wien T2 - Microchimica Acta T1 - Myeloperoxidase-mediated oxidation of organophosphorus pesticides as a pre-step in their determination by AChE based bioanalytical methods EP - 297 IS - 3-4 SP - 289 VL - 170 DO - 10.1007/s00604-010-0324-2 ER -
@article{ author = "Lazarević-Pašti, Tamara and Momić, Tatjana and Onjia, Antonije and Vujisić, Ljubodrag V. and Vasić, Vesna M.", year = "2010", abstract = "In order to improve the sensitivity of assays for inhibitors of the enzyme acetylcholine esterase (AChE), an effective method was developed for the conversion of the organophosphate pesticides (OPs) diazinon, malathion, chlorpyrifos, azinphos-methyl and phorate into more toxic inhibitors. This was accomplished by converting them from the thio form into their oxo form using the enzyme myeloperoxidase. The oxo forms, which are the only products of conversion, were determined by AChE bioassays, using either the free enzyme, or a flow injection analysis manifold with immobilized AChE and spectrophotometric detection. All modified OPs exhibited inhibitory power at ppb levels and within 10 min. The method is considered to represent an excellent means for improving the sensitivity of assays for determination of OPs.", publisher = "Springer Wien, Wien", journal = "Microchimica Acta", title = "Myeloperoxidase-mediated oxidation of organophosphorus pesticides as a pre-step in their determination by AChE based bioanalytical methods", pages = "297-289", number = "3-4", volume = "170", doi = "10.1007/s00604-010-0324-2" }
Lazarević-Pašti, T., Momić, T., Onjia, A., Vujisić, L. V.,& Vasić, V. M.. (2010). Myeloperoxidase-mediated oxidation of organophosphorus pesticides as a pre-step in their determination by AChE based bioanalytical methods. in Microchimica Acta Springer Wien, Wien., 170(3-4), 289-297. https://doi.org/10.1007/s00604-010-0324-2
Lazarević-Pašti T, Momić T, Onjia A, Vujisić LV, Vasić VM. Myeloperoxidase-mediated oxidation of organophosphorus pesticides as a pre-step in their determination by AChE based bioanalytical methods. in Microchimica Acta. 2010;170(3-4):289-297. doi:10.1007/s00604-010-0324-2 .
Lazarević-Pašti, Tamara, Momić, Tatjana, Onjia, Antonije, Vujisić, Ljubodrag V., Vasić, Vesna M., "Myeloperoxidase-mediated oxidation of organophosphorus pesticides as a pre-step in their determination by AChE based bioanalytical methods" in Microchimica Acta, 170, no. 3-4 (2010):289-297, https://doi.org/10.1007/s00604-010-0324-2 . .