Cotton yarn was first oxidized by sodium periodate to introduce aldehyde groups which were able to react with amino groups of trypsin to form Schiff's base, and result in cotton yarn immobilized trypsin. The effect of periodate oxidation on the chemical and physical properties of cotton yarn was evaluated by determining aldehyde group content, fineness and tensile strength of yarn. Measurements of protein load from Bradford assay and catalytic activity in hydrolysis of N-alpha-benzoyl-DL-arginine p-nitroanilide were made for the immobilized enzyme. The maximum amount of immobilized trypsin was 6.1 mg/g dried cotton yarn. Trypsin immobilized on oxidized cotton yarn retained 90% and 72% of the initial activity at 4 degrees C and 25 degrees C, respectively, over 60 days of storage in physiological solution.
TY - JOUR
AU - Nikolić, Tanja
AU - Kostić, Mirjana
AU - Praskalo, Jovana
AU - Pejić, Biljana
AU - Petronijević, Živomir
AU - Škundrić, Petar
PY - 2010
UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1672
AB - Cotton yarn was first oxidized by sodium periodate to introduce aldehyde groups which were able to react with amino groups of trypsin to form Schiff's base, and result in cotton yarn immobilized trypsin. The effect of periodate oxidation on the chemical and physical properties of cotton yarn was evaluated by determining aldehyde group content, fineness and tensile strength of yarn. Measurements of protein load from Bradford assay and catalytic activity in hydrolysis of N-alpha-benzoyl-DL-arginine p-nitroanilide were made for the immobilized enzyme. The maximum amount of immobilized trypsin was 6.1 mg/g dried cotton yarn. Trypsin immobilized on oxidized cotton yarn retained 90% and 72% of the initial activity at 4 degrees C and 25 degrees C, respectively, over 60 days of storage in physiological solution.
PB - Elsevier Sci Ltd, Oxford
T2 - Carbohydrate Polymers
T1 - Sodium periodate oxidized cotton yarn as carrier for immobilization of trypsin
EP - 981
IS - 3
SP - 976
VL - 82
DO - 10.1016/j.carbpol.2010.06.028
ER -
@article{
author = "Nikolić, Tanja and Kostić, Mirjana and Praskalo, Jovana and Pejić, Biljana and Petronijević, Živomir and Škundrić, Petar",
year = "2010",
abstract = "Cotton yarn was first oxidized by sodium periodate to introduce aldehyde groups which were able to react with amino groups of trypsin to form Schiff's base, and result in cotton yarn immobilized trypsin. The effect of periodate oxidation on the chemical and physical properties of cotton yarn was evaluated by determining aldehyde group content, fineness and tensile strength of yarn. Measurements of protein load from Bradford assay and catalytic activity in hydrolysis of N-alpha-benzoyl-DL-arginine p-nitroanilide were made for the immobilized enzyme. The maximum amount of immobilized trypsin was 6.1 mg/g dried cotton yarn. Trypsin immobilized on oxidized cotton yarn retained 90% and 72% of the initial activity at 4 degrees C and 25 degrees C, respectively, over 60 days of storage in physiological solution.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Carbohydrate Polymers",
title = "Sodium periodate oxidized cotton yarn as carrier for immobilization of trypsin",
pages = "981-976",
number = "3",
volume = "82",
doi = "10.1016/j.carbpol.2010.06.028"
}
Nikolić, T., Kostić, M., Praskalo, J., Pejić, B., Petronijević, Ž.,& Škundrić, P.. (2010). Sodium periodate oxidized cotton yarn as carrier for immobilization of trypsin. in Carbohydrate Polymers
Elsevier Sci Ltd, Oxford., 82(3), 976-981.
https://doi.org/10.1016/j.carbpol.2010.06.028
Nikolić T, Kostić M, Praskalo J, Pejić B, Petronijević Ž, Škundrić P. Sodium periodate oxidized cotton yarn as carrier for immobilization of trypsin. in Carbohydrate Polymers. 2010;82(3):976-981.
doi:10.1016/j.carbpol.2010.06.028 .
Nikolić, Tanja, Kostić, Mirjana, Praskalo, Jovana, Pejić, Biljana, Petronijević, Živomir, Škundrić, Petar, "Sodium periodate oxidized cotton yarn as carrier for immobilization of trypsin" in Carbohydrate Polymers, 82, no. 3 (2010):976-981,
https://doi.org/10.1016/j.carbpol.2010.06.028 . .
