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dc.creatorRajilić-Stojanović, Mirjana
dc.creatorMaathuis, Annet
dc.creatorHeilig, Hans G. H. J.
dc.creatorVenema, Koen
dc.creatorde Vos, Willem M.
dc.creatorSmidt, Hauke
dc.date.accessioned2021-03-10T11:24:21Z
dc.date.available2021-03-10T11:24:21Z
dc.date.issued2010
dc.identifier.issn1350-0872
dc.identifier.urihttp://TechnoRep.tmf.bg.ac.rs/handle/123456789/1720
dc.description.abstractA high-density phylogenetic microarray targeting small subunit rRNA (SSU rRNA) sequences of over 1000 microbial phylotypes of the human gastrointestinal tract, the HITChip, was used to assess the impact of faecal inoculum preparation and operation conditions on an in vitro model of the human large intestine (TIM-2). This revealed that propagation of mixed faecal donations for the production of standardized inocula has only a limited effect on the microbiota composition, with slight changes observed mainly within the Firmicutes. Adversely, significant shifts in several major groups of intestinal microbiota were observed after inoculation of the in vitro model. Hierarchical cluster analysis was able to show that samples taken throughout the inoculum preparation grouped with microbiota profiles observed for faecal samples of healthy adults. In contrast, the TIM-2 microbiota was distinct. While members of the Bacteroidetes and some groups within the Bacilli were increased in TIM-2 microbiota, a strong reduction in the relative abundance of other microbial groups, including Bifidobacterium spp., Streptococcus spp., and Clostridium clusters IV and XlVa, was observed. The changes detected with the HITChip could be confirmed using denaturing gradient gel electrophoresis (DGGE) of SSU rRNA amplicons.en
dc.publisherMicrobiology Soc, London
dc.rightsopenAccess
dc.sourceMicrobiology-Sgm
dc.titleEvaluating the microbial diversity of an in vitro model of the human large intestine by phylogenetic microarray analysisen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage3281
dc.citation.other156: 3270-3281
dc.citation.rankM22
dc.citation.spage3270
dc.citation.volume156
dc.identifier.doi10.1099/mic.0.042044-0
dc.identifier.fulltexthttp://TechnoRep.tmf.bg.ac.rs/bitstream/id/317/1717.pdf
dc.identifier.pmid20847013
dc.identifier.rcubconv_3493
dc.identifier.scopus2-s2.0-78049502175
dc.identifier.wos000284660400008
dc.type.versionpublishedVersion


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