Polyaniline (PANI) enzyme electrode was formed by immobilisation of Glucose oxidase (GOx) via glutaraldehyde into electrochemically polymerised PANI on graphite electrode. Electrochemical polymerisation of PANI on graphite was performed from aqueous solution of 1.0 mol dm(-3) HCl and 0.25 mol dm(-3) aniline at constant current density of 2.0 mA cm(-2). Hronopotentiometric curves of the PANI enzyme electrode obtained at current density of 10 mu A cm(-2) were recorded in different glucose concentrations. The linearity response range was between 1.0 and 5.0 mmol dm(-3) of glucose concentration. The estimated apparent Michaelis-Menten constant, was K-m(') = 0.30 mmol dm(-3), which is significantly lower than that of free enzyme.
TY - JOUR
AU - Gvozdenović, Milica
AU - Jugović, Branimir
AU - Bezbradica, Dejan
AU - Antov, Mirjana
AU - Knežević-Jugović, Zorica
AU - Grgur, Branimir
PY - 2011
UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1826
AB - Polyaniline (PANI) enzyme electrode was formed by immobilisation of Glucose oxidase (GOx) via glutaraldehyde into electrochemically polymerised PANI on graphite electrode. Electrochemical polymerisation of PANI on graphite was performed from aqueous solution of 1.0 mol dm(-3) HCl and 0.25 mol dm(-3) aniline at constant current density of 2.0 mA cm(-2). Hronopotentiometric curves of the PANI enzyme electrode obtained at current density of 10 mu A cm(-2) were recorded in different glucose concentrations. The linearity response range was between 1.0 and 5.0 mmol dm(-3) of glucose concentration. The estimated apparent Michaelis-Menten constant, was K-m(') = 0.30 mmol dm(-3), which is significantly lower than that of free enzyme.
PB - Elsevier Sci Ltd, Oxford
T2 - Food Chemistry
T1 - Electrochemical determination of glucose using polyaniline electrode modified by glucose oxidase
EP - 400
IS - 1
SP - 396
VL - 124
DO - 10.1016/j.foodchem.2010.06.046
ER -
@article{
author = "Gvozdenović, Milica and Jugović, Branimir and Bezbradica, Dejan and Antov, Mirjana and Knežević-Jugović, Zorica and Grgur, Branimir",
year = "2011",
abstract = "Polyaniline (PANI) enzyme electrode was formed by immobilisation of Glucose oxidase (GOx) via glutaraldehyde into electrochemically polymerised PANI on graphite electrode. Electrochemical polymerisation of PANI on graphite was performed from aqueous solution of 1.0 mol dm(-3) HCl and 0.25 mol dm(-3) aniline at constant current density of 2.0 mA cm(-2). Hronopotentiometric curves of the PANI enzyme electrode obtained at current density of 10 mu A cm(-2) were recorded in different glucose concentrations. The linearity response range was between 1.0 and 5.0 mmol dm(-3) of glucose concentration. The estimated apparent Michaelis-Menten constant, was K-m(') = 0.30 mmol dm(-3), which is significantly lower than that of free enzyme.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Food Chemistry",
title = "Electrochemical determination of glucose using polyaniline electrode modified by glucose oxidase",
pages = "400-396",
number = "1",
volume = "124",
doi = "10.1016/j.foodchem.2010.06.046"
}
Gvozdenović, M., Jugović, B., Bezbradica, D., Antov, M., Knežević-Jugović, Z.,& Grgur, B.. (2011). Electrochemical determination of glucose using polyaniline electrode modified by glucose oxidase. in Food Chemistry
Elsevier Sci Ltd, Oxford., 124(1), 396-400.
https://doi.org/10.1016/j.foodchem.2010.06.046
Gvozdenović M, Jugović B, Bezbradica D, Antov M, Knežević-Jugović Z, Grgur B. Electrochemical determination of glucose using polyaniline electrode modified by glucose oxidase. in Food Chemistry. 2011;124(1):396-400.
doi:10.1016/j.foodchem.2010.06.046 .
Gvozdenović, Milica, Jugović, Branimir, Bezbradica, Dejan, Antov, Mirjana, Knežević-Jugović, Zorica, Grgur, Branimir, "Electrochemical determination of glucose using polyaniline electrode modified by glucose oxidase" in Food Chemistry, 124, no. 1 (2011):396-400,
https://doi.org/10.1016/j.foodchem.2010.06.046 . .
