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dc.creatorKnežević, Zorica
dc.creatorŠiler-Marinković, Slavica
dc.creatorMojović, Ljiljana
dc.date.accessioned2021-03-10T09:44:40Z
dc.date.available2021-03-10T09:44:40Z
dc.date.issued1998
dc.identifier.issn0175-7598
dc.identifier.urihttp://TechnoRep.tmf.bg.ac.rs/handle/123456789/187
dc.description.abstractCandida rugosa lipase has been used to investigate the hydrolysis of palm oil in a lecithin/isooctane reversed micellar system. The reaction obeys Michaelis-Menten kinetics for the initial conditions. Kinetic parameters such as maximum rate and Michaelis constant (K-m) were determined for lipase-catalyzed hydrolysis in n-hexane and isooctane. According to the K-m values, the enzyme affinity towards the substrate was increased in isooctane. The maximum degree of hydrolysis was generally decreased as the initial substrate concentration was increased. This may suggest that the hydrolysis in lecithin reversed micelles should be regarded as a one-substrate first-order reversible reaction. It is shown in this study that the proposed one-substrate first-order kinetic model can serve for the precise prediction of the degree of hydrolysis for a known reaction time or vice versa, when the initial substrate concentration is less than 0.325 mol/dm(3). A disagreement with this model was found when the initial substrate concentration was higher than approximately 0.3 mol/dm(3). This may be due to the effects of the products on lipase activity or even to the conversion of the reversed micellar system to other systems.en
dc.publisherSpringer, New York
dc.rightsrestrictedAccess
dc.sourceApplied Microbiology and Biotechnology
dc.titleKinetics of lipase-catalyzed hydrolysis of palm oil in lecithin/izooctane reversed micelllesen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage271
dc.citation.issue3
dc.citation.other49(3): 267-271
dc.citation.rankM22
dc.citation.spage267
dc.citation.volume49
dc.identifier.doi10.1007/s002530051167
dc.identifier.rcubconv_1868
dc.identifier.scopus2-s2.0-0031893209
dc.identifier.wos000073117700004
dc.type.versionpublishedVersion


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