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dc.creatorVeličković, Dušan
dc.creatorDimitrijević, Aleksandra
dc.creatorBihelović, Filip
dc.creatorBezbradica, Dejan
dc.creatorJankov, Ratko
dc.creatorMilosavić, Nenad
dc.date.accessioned2021-03-10T11:35:22Z
dc.date.available2021-03-10T11:35:22Z
dc.date.issued2011
dc.identifier.issn1359-5113
dc.identifier.urihttp://TechnoRep.tmf.bg.ac.rs/handle/123456789/1891
dc.description.abstractIn this report, alpha-isosalicin, a potent anticoagulant and skin whitening agent, was synthesized by a highly efficient chemoselective and diastereoselective reaction, catalyzed by maltase from bakers' yeast (Saccharomyces cerevisiae). The highest yield of this one-step transglucosylation reaction was achieved with 50 mM of salicyl alcohol as a glucose acceptor. The key reaction factors were optimized using response surface methodology (RSM) with an enzyme concentration of 10 U/mL. The optimum temperature of the reaction was determined as 36.5 degrees C, the optimal maltose concentration was 40% (w/v), the optimal pH was 6.5, and the optimal reaction time was 16 h. Under these conditions 75% of alpha-isosalicin was obtained, with a yield of 10 g/L, and no by product formation was observed.en
dc.publisherElsevier Sci Ltd, Oxford
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172049/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46010/RS//
dc.rightsrestrictedAccess
dc.sourceProcess Biochemistry
dc.subjectalpha-Isosalicinen
dc.subjectGlucosidaseen
dc.subjectSalicyl alcoholen
dc.subjectTransglucosylationen
dc.subjectBakers' yeasten
dc.titleA highly efficient diastereoselective synthesis of alpha-isosalicin by maltase from Saccharomyces cerevisiaeen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage1702
dc.citation.issue8
dc.citation.other46(8): 1698-1702
dc.citation.rankM21
dc.citation.spage1698
dc.citation.volume46
dc.identifier.doi10.1016/j.procbio.2011.05.007
dc.identifier.rcubconv_3639
dc.identifier.scopus2-s2.0-79959922337
dc.identifier.wos000293438300025
dc.type.versionpublishedVersion


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