Production of lipase and protease from an indigenous Pseudomonas aeruginosa strain and their evaluation as detergent additives: Compatibility study with detergent ingredients and washing performance
Само за регистроване кориснике
2011
Аутори
Grbavčić, SanjaBezbradica, Dejan
Izrael-Zivković, Lidija
Avramović, Nataša
Milosavić, Nenad
Karadžić, Ivanka
Knežević-Jugović, Zorica
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
An indigenous Pseudomonas aeruginosa strain has been studied for lipase and protease activities for their potential application in detergents. Produced enzymes were investigated in order to assess their compatibility with several surfactants, oxidizing agents and commercial detergents. The crude lipase appeared to retain high activity and stability in the presence of several surfactants and oxidizing agents and it was insusceptible to proteolysis. Lutensol (R) XP80 and Triton (R) X-100 strongly activated the lipase for a long period (up to 40 and 30% against the control after 1 h) while the protease activity was enhanced by the addition of Triton (R) WR1339 and Tween (R) 80. The washing performance of the investigated surfactants was significantly improved with the addition of the crude enzyme preparation. Studies were further undertaken to improve enzymes production. The optimization of fermentation conditions led to an 8-fold increase of lipase production, while the production of pro...tease was enhanced by 60%.
Кључне речи:
Pseudomonas aeruginosa / Lipase / Protease / Detergent / Medium optimizationИзвор:
Bioresource Technology, 2011, 102, 24, 11226-11233Издавач:
- Elsevier Sci Ltd, Oxford
Финансирање / пројекти:
- Развој нових инкапсулационих и ензимских технологија за производњу биокатализатора и биолошки активних компонената хране у циљу повећања њене конкурентности, квалитета и безбедности (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46010)
DOI: 10.1016/j.biortech.2011.09.076
ISSN: 0960-8524
PubMed: 22004595
WoS: 000298127600026
Scopus: 2-s2.0-82755177953
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Grbavčić, Sanja AU - Bezbradica, Dejan AU - Izrael-Zivković, Lidija AU - Avramović, Nataša AU - Milosavić, Nenad AU - Karadžić, Ivanka AU - Knežević-Jugović, Zorica PY - 2011 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1895 AB - An indigenous Pseudomonas aeruginosa strain has been studied for lipase and protease activities for their potential application in detergents. Produced enzymes were investigated in order to assess their compatibility with several surfactants, oxidizing agents and commercial detergents. The crude lipase appeared to retain high activity and stability in the presence of several surfactants and oxidizing agents and it was insusceptible to proteolysis. Lutensol (R) XP80 and Triton (R) X-100 strongly activated the lipase for a long period (up to 40 and 30% against the control after 1 h) while the protease activity was enhanced by the addition of Triton (R) WR1339 and Tween (R) 80. The washing performance of the investigated surfactants was significantly improved with the addition of the crude enzyme preparation. Studies were further undertaken to improve enzymes production. The optimization of fermentation conditions led to an 8-fold increase of lipase production, while the production of protease was enhanced by 60%. PB - Elsevier Sci Ltd, Oxford T2 - Bioresource Technology T1 - Production of lipase and protease from an indigenous Pseudomonas aeruginosa strain and their evaluation as detergent additives: Compatibility study with detergent ingredients and washing performance EP - 11233 IS - 24 SP - 11226 VL - 102 DO - 10.1016/j.biortech.2011.09.076 ER -
@article{ author = "Grbavčić, Sanja and Bezbradica, Dejan and Izrael-Zivković, Lidija and Avramović, Nataša and Milosavić, Nenad and Karadžić, Ivanka and Knežević-Jugović, Zorica", year = "2011", abstract = "An indigenous Pseudomonas aeruginosa strain has been studied for lipase and protease activities for their potential application in detergents. Produced enzymes were investigated in order to assess their compatibility with several surfactants, oxidizing agents and commercial detergents. The crude lipase appeared to retain high activity and stability in the presence of several surfactants and oxidizing agents and it was insusceptible to proteolysis. Lutensol (R) XP80 and Triton (R) X-100 strongly activated the lipase for a long period (up to 40 and 30% against the control after 1 h) while the protease activity was enhanced by the addition of Triton (R) WR1339 and Tween (R) 80. The washing performance of the investigated surfactants was significantly improved with the addition of the crude enzyme preparation. Studies were further undertaken to improve enzymes production. The optimization of fermentation conditions led to an 8-fold increase of lipase production, while the production of protease was enhanced by 60%.", publisher = "Elsevier Sci Ltd, Oxford", journal = "Bioresource Technology", title = "Production of lipase and protease from an indigenous Pseudomonas aeruginosa strain and their evaluation as detergent additives: Compatibility study with detergent ingredients and washing performance", pages = "11233-11226", number = "24", volume = "102", doi = "10.1016/j.biortech.2011.09.076" }
Grbavčić, S., Bezbradica, D., Izrael-Zivković, L., Avramović, N., Milosavić, N., Karadžić, I.,& Knežević-Jugović, Z.. (2011). Production of lipase and protease from an indigenous Pseudomonas aeruginosa strain and their evaluation as detergent additives: Compatibility study with detergent ingredients and washing performance. in Bioresource Technology Elsevier Sci Ltd, Oxford., 102(24), 11226-11233. https://doi.org/10.1016/j.biortech.2011.09.076
Grbavčić S, Bezbradica D, Izrael-Zivković L, Avramović N, Milosavić N, Karadžić I, Knežević-Jugović Z. Production of lipase and protease from an indigenous Pseudomonas aeruginosa strain and their evaluation as detergent additives: Compatibility study with detergent ingredients and washing performance. in Bioresource Technology. 2011;102(24):11226-11233. doi:10.1016/j.biortech.2011.09.076 .
Grbavčić, Sanja, Bezbradica, Dejan, Izrael-Zivković, Lidija, Avramović, Nataša, Milosavić, Nenad, Karadžić, Ivanka, Knežević-Jugović, Zorica, "Production of lipase and protease from an indigenous Pseudomonas aeruginosa strain and their evaluation as detergent additives: Compatibility study with detergent ingredients and washing performance" in Bioresource Technology, 102, no. 24 (2011):11226-11233, https://doi.org/10.1016/j.biortech.2011.09.076 . .