The objective of this paper was the investigation of a suitable Sepabeads(A (R)) support and method for immobilization of lipase from Candida rugosa. Three different supports were used, two with amino groups, (Sepabeads(A (R)) EC-EA and Sepabeads(A (R)) EC-HA), differing in spacer length (two and six carbons, respectively) and one with epoxy group (Sepabeads(A (R)) EC-EP). Lipase immobilization was carried out by two conventional methods (via epoxy groups and via glutaraldehyde), and with periodate method for modification of lipase. The results of activity assays showed that lipase retained 94.8% or 87.6% of activity after immobilization via epoxy groups or with periodate method, respectively, while glutaraldehyde method was inferior with only 12.7% of retention. The immobilization of lipase, previously modified by periodate oxidation, via amino groups has proven to be more efficient than direct immobilization of lipase via epoxy groups. In such a way immobilized enzyme exhibited highe...r activity at high reaction temperatures and higher thermal stability.
TY - JOUR
AU - Prlainović, Nevena
AU - Knežević-Jugović, Zorica
AU - Mijin, Dušan
AU - Bezbradica, Dejan
PY - 2011
UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1939
AB - The objective of this paper was the investigation of a suitable Sepabeads(A (R)) support and method for immobilization of lipase from Candida rugosa. Three different supports were used, two with amino groups, (Sepabeads(A (R)) EC-EA and Sepabeads(A (R)) EC-HA), differing in spacer length (two and six carbons, respectively) and one with epoxy group (Sepabeads(A (R)) EC-EP). Lipase immobilization was carried out by two conventional methods (via epoxy groups and via glutaraldehyde), and with periodate method for modification of lipase. The results of activity assays showed that lipase retained 94.8% or 87.6% of activity after immobilization via epoxy groups or with periodate method, respectively, while glutaraldehyde method was inferior with only 12.7% of retention. The immobilization of lipase, previously modified by periodate oxidation, via amino groups has proven to be more efficient than direct immobilization of lipase via epoxy groups. In such a way immobilized enzyme exhibited higher activity at high reaction temperatures and higher thermal stability.
PB - Springer, New York
T2 - Bioprocess and Biosystems Engineering
T1 - Immobilization of lipase from Candida rugosa on Sepabeads(A (R)): the effect of lipase oxidation by periodates
EP - 810
IS - 7
SP - 803
VL - 34
DO - 10.1007/s00449-011-0530-2
ER -
@article{
author = "Prlainović, Nevena and Knežević-Jugović, Zorica and Mijin, Dušan and Bezbradica, Dejan",
year = "2011",
abstract = "The objective of this paper was the investigation of a suitable Sepabeads(A (R)) support and method for immobilization of lipase from Candida rugosa. Three different supports were used, two with amino groups, (Sepabeads(A (R)) EC-EA and Sepabeads(A (R)) EC-HA), differing in spacer length (two and six carbons, respectively) and one with epoxy group (Sepabeads(A (R)) EC-EP). Lipase immobilization was carried out by two conventional methods (via epoxy groups and via glutaraldehyde), and with periodate method for modification of lipase. The results of activity assays showed that lipase retained 94.8% or 87.6% of activity after immobilization via epoxy groups or with periodate method, respectively, while glutaraldehyde method was inferior with only 12.7% of retention. The immobilization of lipase, previously modified by periodate oxidation, via amino groups has proven to be more efficient than direct immobilization of lipase via epoxy groups. In such a way immobilized enzyme exhibited higher activity at high reaction temperatures and higher thermal stability.",
publisher = "Springer, New York",
journal = "Bioprocess and Biosystems Engineering",
title = "Immobilization of lipase from Candida rugosa on Sepabeads(A (R)): the effect of lipase oxidation by periodates",
pages = "810-803",
number = "7",
volume = "34",
doi = "10.1007/s00449-011-0530-2"
}
Prlainović, N., Knežević-Jugović, Z., Mijin, D.,& Bezbradica, D.. (2011). Immobilization of lipase from Candida rugosa on Sepabeads(A (R)): the effect of lipase oxidation by periodates. in Bioprocess and Biosystems Engineering
Springer, New York., 34(7), 803-810.
https://doi.org/10.1007/s00449-011-0530-2
Prlainović N, Knežević-Jugović Z, Mijin D, Bezbradica D. Immobilization of lipase from Candida rugosa on Sepabeads(A (R)): the effect of lipase oxidation by periodates. in Bioprocess and Biosystems Engineering. 2011;34(7):803-810.
doi:10.1007/s00449-011-0530-2 .
Prlainović, Nevena, Knežević-Jugović, Zorica, Mijin, Dušan, Bezbradica, Dejan, "Immobilization of lipase from Candida rugosa on Sepabeads(A (R)): the effect of lipase oxidation by periodates" in Bioprocess and Biosystems Engineering, 34, no. 7 (2011):803-810,
https://doi.org/10.1007/s00449-011-0530-2 . .
