Application of alginates in cell and enzyme immobilization
Nema prikaza
Autori
Milosavić, NenadDimitrijević, Aleksandra
Veličković, Dušan
Bezbradica, Dejan
Knežević-Jugović, Zorica
Jankov, Ratko
Poglavlje u monografiji (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Enzymes are a particularly versatile class of catalysts that perform and regulate processes in living matter. Enzymatic regio-, chemo- and enantioselectivity were used in industry and in organic synthesis. The common perception is however, that enzymes are sensitive, unstable and have to be used in water, features that are not ideal for a catalyst and undesirable in most syntheses. In many cases a way to avoid at least part of these complaints is to immobilize enzymes. There are several immobilization techniques, and the best means of avoiding any negative influence on the structure of an enzyme is to encapsulate it. Due to its ability to form gel with multivalent cations under relatively mild conditions, alginates are very important in cell and enzyme encapsulation. Entrapment within insoluble alginate gel is recognized as a rapid, nontoxic, inexpensive and versatile method for immobilization of enzymes as well as cells. The resultant gel is biochemically inert and mechanically stable... with interstitial spaces that are suitable for cell immobilization.
Izvor:
Alginates: Production, Types and Applications, 2012, 37-60Institucija/grupa
Tehnološko-metalurški fakultetTY - CHAP AU - Milosavić, Nenad AU - Dimitrijević, Aleksandra AU - Veličković, Dušan AU - Bezbradica, Dejan AU - Knežević-Jugović, Zorica AU - Jankov, Ratko PY - 2012 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1968 AB - Enzymes are a particularly versatile class of catalysts that perform and regulate processes in living matter. Enzymatic regio-, chemo- and enantioselectivity were used in industry and in organic synthesis. The common perception is however, that enzymes are sensitive, unstable and have to be used in water, features that are not ideal for a catalyst and undesirable in most syntheses. In many cases a way to avoid at least part of these complaints is to immobilize enzymes. There are several immobilization techniques, and the best means of avoiding any negative influence on the structure of an enzyme is to encapsulate it. Due to its ability to form gel with multivalent cations under relatively mild conditions, alginates are very important in cell and enzyme encapsulation. Entrapment within insoluble alginate gel is recognized as a rapid, nontoxic, inexpensive and versatile method for immobilization of enzymes as well as cells. The resultant gel is biochemically inert and mechanically stable with interstitial spaces that are suitable for cell immobilization. T2 - Alginates: Production, Types and Applications T1 - Application of alginates in cell and enzyme immobilization EP - 60 SP - 37 UR - https://hdl.handle.net/21.15107/rcub_technorep_1968 ER -
@inbook{ author = "Milosavić, Nenad and Dimitrijević, Aleksandra and Veličković, Dušan and Bezbradica, Dejan and Knežević-Jugović, Zorica and Jankov, Ratko", year = "2012", abstract = "Enzymes are a particularly versatile class of catalysts that perform and regulate processes in living matter. Enzymatic regio-, chemo- and enantioselectivity were used in industry and in organic synthesis. The common perception is however, that enzymes are sensitive, unstable and have to be used in water, features that are not ideal for a catalyst and undesirable in most syntheses. In many cases a way to avoid at least part of these complaints is to immobilize enzymes. There are several immobilization techniques, and the best means of avoiding any negative influence on the structure of an enzyme is to encapsulate it. Due to its ability to form gel with multivalent cations under relatively mild conditions, alginates are very important in cell and enzyme encapsulation. Entrapment within insoluble alginate gel is recognized as a rapid, nontoxic, inexpensive and versatile method for immobilization of enzymes as well as cells. The resultant gel is biochemically inert and mechanically stable with interstitial spaces that are suitable for cell immobilization.", journal = "Alginates: Production, Types and Applications", booktitle = "Application of alginates in cell and enzyme immobilization", pages = "60-37", url = "https://hdl.handle.net/21.15107/rcub_technorep_1968" }
Milosavić, N., Dimitrijević, A., Veličković, D., Bezbradica, D., Knežević-Jugović, Z.,& Jankov, R.. (2012). Application of alginates in cell and enzyme immobilization. in Alginates: Production, Types and Applications, 37-60. https://hdl.handle.net/21.15107/rcub_technorep_1968
Milosavić N, Dimitrijević A, Veličković D, Bezbradica D, Knežević-Jugović Z, Jankov R. Application of alginates in cell and enzyme immobilization. in Alginates: Production, Types and Applications. 2012;:37-60. https://hdl.handle.net/21.15107/rcub_technorep_1968 .
Milosavić, Nenad, Dimitrijević, Aleksandra, Veličković, Dušan, Bezbradica, Dejan, Knežević-Jugović, Zorica, Jankov, Ratko, "Application of alginates in cell and enzyme immobilization" in Alginates: Production, Types and Applications (2012):37-60, https://hdl.handle.net/21.15107/rcub_technorep_1968 .