TechnoRep - Faculty of Technology and Metallurgy Repository
University of Belgrade - Faculty of Technology and Metallurgy
    • English
    • Српски
    • Српски (Serbia)
  • English 
    • English
    • Serbian (Cyrillic)
    • Serbian (Latin)
  • Login
View Item 
  •   TechnoRep
  • Tehnološko-metalurški fakultet
  • Radovi istraživača / Researchers’ publications (TMF)
  • View Item
  •   TechnoRep
  • Tehnološko-metalurški fakultet
  • Radovi istraživača / Researchers’ publications (TMF)
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Study of lipase-catalyzed synthesis of ascorbyl oleate using response surface methodology

No Thumbnail
Authors
Stojanović, M.M.
Veličković, Dušan
Dimitrijević, Aleksandra
Milosavić, Nenad
Knežević-Jugović, Zorica
Bezbradica, Dejan
Conference object (Published version)
Metadata
Show full item record
Abstract
L-ascorbic acid has good antioxidative properties but its efficiency in stabilizing fats and oils in products with high lipid content is negligible due to its hydrophilic characteristics. On the other hand, fatty acid ascorbyl esters are liposoluble, with even better antioxidative properties comparing to L-ascorbic acid. Therefore, developing of industrial process for lipase-catalyzed synthesis of vitamin C fatty acid esters, considering numerous advantages over conventional chemical methods (mild reaction conditions, high regioselectivity, and simplified downstream processing), is of great interest. In this study, L-ascorbyl oleate was synthesized in esterification reaction between vitamin C and oleic acid catalyzed by immobilized lipase from Candida antarctica in acetone as a reaction medium. Response surface methodology and 5-level-5-factor central composite rotatable design were employed in order to investigate interactions between experimental factors (initial water content, tempe...rature, substrates molar ratio, vitamin C concentration, and enzyme amount), determine their individual influence on molar conversion, and eventually optimize the synthesis. Based on the experimental data, regression model, expressed with second order polynomial equation, was obtained. At values in the range of examination, enzyme amount had no influence on conversion so it was fixed at the minimum (0.2 % (w/v)). The maximum molar conversion of 91.3 % was predicted and corresponding, optimal reaction conditions were: temperature - 60 °C, initial water content - 0 % (v/v), vitamin C concentration - 0.02 M, and substrate molar ratio - 1:15. Our system provided reaction conditions which enabled high conversions, thus obtained results may be used as a starting point for the process scale-up.

Keywords:
Ascorbyl oleate / Lipase / Optimization / response surface methodology (RSM)
Source:
CEFood 2012 - Proceedings of 6th Central European Congress on Food, 2012, 807-813
Publisher:
  • 6th Central European Congress on Food, CEFood 2012

ISBN: 978-867994027-8

PubMed:

Scopus: 2-s2.0-84926010053
[ Google Scholar ]
Handle
https://hdl.handle.net/21.15107/rcub_technorep_1976
URI
http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1976
Collections
  • Radovi istraživača / Researchers’ publications (TMF)
Institution/Community
Tehnološko-metalurški fakultet
TY  - CONF
AU  - Stojanović, M.M.
AU  - Veličković, Dušan
AU  - Dimitrijević, Aleksandra
AU  - Milosavić, Nenad
AU  - Knežević-Jugović, Zorica
AU  - Bezbradica, Dejan
PY  - 2012
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1976
AB  - L-ascorbic acid has good antioxidative properties but its efficiency in stabilizing fats and oils in products with high lipid content is negligible due to its hydrophilic characteristics. On the other hand, fatty acid ascorbyl esters are liposoluble, with even better antioxidative properties comparing to L-ascorbic acid. Therefore, developing of industrial process for lipase-catalyzed synthesis of vitamin C fatty acid esters, considering numerous advantages over conventional chemical methods (mild reaction conditions, high regioselectivity, and simplified downstream processing), is of great interest. In this study, L-ascorbyl oleate was synthesized in esterification reaction between vitamin C and oleic acid catalyzed by immobilized lipase from Candida antarctica in acetone as a reaction medium. Response surface methodology and 5-level-5-factor central composite rotatable design were employed in order to investigate interactions between experimental factors (initial water content, temperature, substrates molar ratio, vitamin C concentration, and enzyme amount), determine their individual influence on molar conversion, and eventually optimize the synthesis. Based on the experimental data, regression model, expressed with second order polynomial equation, was obtained. At values in the range of examination, enzyme amount had no influence on conversion so it was fixed at the minimum (0.2 % (w/v)). The maximum molar conversion of 91.3 % was predicted and corresponding, optimal reaction conditions were: temperature - 60 °C, initial water content - 0 % (v/v), vitamin C concentration - 0.02 M, and substrate molar ratio - 1:15. Our system provided reaction conditions which enabled high conversions, thus obtained results may be used as a starting point for the process scale-up.
PB  - 6th Central European Congress on Food, CEFood 2012
C3  - CEFood 2012 - Proceedings of 6th Central European Congress on Food
T1  - Study of lipase-catalyzed synthesis of ascorbyl oleate using response surface methodology
EP  - 813
SP  - 807
UR  - https://hdl.handle.net/21.15107/rcub_technorep_1976
ER  - 
@conference{
author = "Stojanović, M.M. and Veličković, Dušan and Dimitrijević, Aleksandra and Milosavić, Nenad and Knežević-Jugović, Zorica and Bezbradica, Dejan",
year = "2012",
abstract = "L-ascorbic acid has good antioxidative properties but its efficiency in stabilizing fats and oils in products with high lipid content is negligible due to its hydrophilic characteristics. On the other hand, fatty acid ascorbyl esters are liposoluble, with even better antioxidative properties comparing to L-ascorbic acid. Therefore, developing of industrial process for lipase-catalyzed synthesis of vitamin C fatty acid esters, considering numerous advantages over conventional chemical methods (mild reaction conditions, high regioselectivity, and simplified downstream processing), is of great interest. In this study, L-ascorbyl oleate was synthesized in esterification reaction between vitamin C and oleic acid catalyzed by immobilized lipase from Candida antarctica in acetone as a reaction medium. Response surface methodology and 5-level-5-factor central composite rotatable design were employed in order to investigate interactions between experimental factors (initial water content, temperature, substrates molar ratio, vitamin C concentration, and enzyme amount), determine their individual influence on molar conversion, and eventually optimize the synthesis. Based on the experimental data, regression model, expressed with second order polynomial equation, was obtained. At values in the range of examination, enzyme amount had no influence on conversion so it was fixed at the minimum (0.2 % (w/v)). The maximum molar conversion of 91.3 % was predicted and corresponding, optimal reaction conditions were: temperature - 60 °C, initial water content - 0 % (v/v), vitamin C concentration - 0.02 M, and substrate molar ratio - 1:15. Our system provided reaction conditions which enabled high conversions, thus obtained results may be used as a starting point for the process scale-up.",
publisher = "6th Central European Congress on Food, CEFood 2012",
journal = "CEFood 2012 - Proceedings of 6th Central European Congress on Food",
title = "Study of lipase-catalyzed synthesis of ascorbyl oleate using response surface methodology",
pages = "813-807",
url = "https://hdl.handle.net/21.15107/rcub_technorep_1976"
}
Stojanović, M.M., Veličković, D., Dimitrijević, A., Milosavić, N., Knežević-Jugović, Z.,& Bezbradica, D.. (2012). Study of lipase-catalyzed synthesis of ascorbyl oleate using response surface methodology. in CEFood 2012 - Proceedings of 6th Central European Congress on Food
6th Central European Congress on Food, CEFood 2012., 807-813.
https://hdl.handle.net/21.15107/rcub_technorep_1976
Stojanović M, Veličković D, Dimitrijević A, Milosavić N, Knežević-Jugović Z, Bezbradica D. Study of lipase-catalyzed synthesis of ascorbyl oleate using response surface methodology. in CEFood 2012 - Proceedings of 6th Central European Congress on Food. 2012;:807-813.
https://hdl.handle.net/21.15107/rcub_technorep_1976 .
Stojanović, M.M., Veličković, Dušan, Dimitrijević, Aleksandra, Milosavić, Nenad, Knežević-Jugović, Zorica, Bezbradica, Dejan, "Study of lipase-catalyzed synthesis of ascorbyl oleate using response surface methodology" in CEFood 2012 - Proceedings of 6th Central European Congress on Food (2012):807-813,
https://hdl.handle.net/21.15107/rcub_technorep_1976 .

DSpace software copyright © 2002-2015  DuraSpace
About TechnoRep | Send Feedback

OpenAIRERCUB
 

 

All of DSpaceInstitutions/communitiesAuthorsTitlesSubjectsThis institutionAuthorsTitlesSubjects

Statistics

View Usage Statistics

DSpace software copyright © 2002-2015  DuraSpace
About TechnoRep | Send Feedback

OpenAIRERCUB