From Agricultural Waste to Biofuel: Enzymatic Potential of a Bacterial Isolate Streptomyces fulvissimus CKS7 for Bioethanol Production
Authorized Users Only
2021
Authors
Mihajlovski, Katarina
Buntić, Aneta

Milić, Marija

Rajilić-Stojanović, Mirjana

Dimitrijević-Branković, Suzana

Article (Published version)

Metadata
Show full item recordAbstract
Purpose To avoid a negative environmental and economic impact of agricultural wastes, and following the principles of circular economy, the reuse of agricultural wastes is necessary. For this purpose, isolation of novel microorganisms with potential biotechnological application is recommended. The current researches in bioethanol production are aimed to reduce the production costs using low-cost substrates and in-house produced enzymes by novel isolated microorganisms. In line with this, in this study valorization of these agricultural by-products by novel isolate S. fulvissimus CKS7 to biotechnological value added products was done. Methods Standard microbiological methods were used for the isolation and characterization of strain. Enzymes activities were determinated using DNS method while, the ethanol concentration was determined based on the density of the alcohol distillate at 20 degrees C. Results The maximal enzymatic activities for amylase, cellulases (carboxymethyl cellulase a...nd Avicelase), pectinase and xylanase were achieved using rye bran as a waste substrate for CKS7 growth. Obtained crude bacterial enzymes were used for enzymatic hydrolysis of lignocellulosic materials including horsetail waste, yellow gentian waste, corn stover, cotton material and corona pre-treated cotton material. The maximum yield of reducing sugars was obtained on horsetail waste and corona pre-treated cotton material. Waste brewer's yeast Saccharomyces cerevisiae was successfully used for the production of bioethanol using horsetail waste hydrolysate and corona pre-treated cotton material hydrolysate. Conclusion The obtained results showed that bacterial strain CKS7 has a significant, still unexplored enzymatic potential that could be used to achieve a cleaner, environmental friendly and economically acceptable biofuel production. [GRAPHICS] .
Keywords:
Solid state fermentation / Streptomyces fulvissimus CKS7 / Hydrolytic enzymes production / Lignocellulosic waste hydrolysis / BioethanolSource:
Waste and Biomass Valorization, 2021, 12, 1, 165-174Publisher:
- Springer, Dordrecht
Funding / projects:
- Application of biotechnological methods for sustainable exploitation of by-products of agro-industry (RS-31035)
DOI: 10.1007/s12649-020-00960-3
ISSN: 1877-2641
WoS: 000516057100001
Scopus: 2-s2.0-85079461025
Institution/Community
Tehnološko-metalurški fakultetTY - JOUR AU - Mihajlovski, Katarina AU - Buntić, Aneta AU - Milić, Marija AU - Rajilić-Stojanović, Mirjana AU - Dimitrijević-Branković, Suzana PY - 2021 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/20 AB - Purpose To avoid a negative environmental and economic impact of agricultural wastes, and following the principles of circular economy, the reuse of agricultural wastes is necessary. For this purpose, isolation of novel microorganisms with potential biotechnological application is recommended. The current researches in bioethanol production are aimed to reduce the production costs using low-cost substrates and in-house produced enzymes by novel isolated microorganisms. In line with this, in this study valorization of these agricultural by-products by novel isolate S. fulvissimus CKS7 to biotechnological value added products was done. Methods Standard microbiological methods were used for the isolation and characterization of strain. Enzymes activities were determinated using DNS method while, the ethanol concentration was determined based on the density of the alcohol distillate at 20 degrees C. Results The maximal enzymatic activities for amylase, cellulases (carboxymethyl cellulase and Avicelase), pectinase and xylanase were achieved using rye bran as a waste substrate for CKS7 growth. Obtained crude bacterial enzymes were used for enzymatic hydrolysis of lignocellulosic materials including horsetail waste, yellow gentian waste, corn stover, cotton material and corona pre-treated cotton material. The maximum yield of reducing sugars was obtained on horsetail waste and corona pre-treated cotton material. Waste brewer's yeast Saccharomyces cerevisiae was successfully used for the production of bioethanol using horsetail waste hydrolysate and corona pre-treated cotton material hydrolysate. Conclusion The obtained results showed that bacterial strain CKS7 has a significant, still unexplored enzymatic potential that could be used to achieve a cleaner, environmental friendly and economically acceptable biofuel production. [GRAPHICS] . PB - Springer, Dordrecht T2 - Waste and Biomass Valorization T1 - From Agricultural Waste to Biofuel: Enzymatic Potential of a Bacterial Isolate Streptomyces fulvissimus CKS7 for Bioethanol Production EP - 174 IS - 1 SP - 165 VL - 12 DO - 10.1007/s12649-020-00960-3 ER -
@article{ author = "Mihajlovski, Katarina and Buntić, Aneta and Milić, Marija and Rajilić-Stojanović, Mirjana and Dimitrijević-Branković, Suzana", year = "2021", abstract = "Purpose To avoid a negative environmental and economic impact of agricultural wastes, and following the principles of circular economy, the reuse of agricultural wastes is necessary. For this purpose, isolation of novel microorganisms with potential biotechnological application is recommended. The current researches in bioethanol production are aimed to reduce the production costs using low-cost substrates and in-house produced enzymes by novel isolated microorganisms. In line with this, in this study valorization of these agricultural by-products by novel isolate S. fulvissimus CKS7 to biotechnological value added products was done. Methods Standard microbiological methods were used for the isolation and characterization of strain. Enzymes activities were determinated using DNS method while, the ethanol concentration was determined based on the density of the alcohol distillate at 20 degrees C. Results The maximal enzymatic activities for amylase, cellulases (carboxymethyl cellulase and Avicelase), pectinase and xylanase were achieved using rye bran as a waste substrate for CKS7 growth. Obtained crude bacterial enzymes were used for enzymatic hydrolysis of lignocellulosic materials including horsetail waste, yellow gentian waste, corn stover, cotton material and corona pre-treated cotton material. The maximum yield of reducing sugars was obtained on horsetail waste and corona pre-treated cotton material. Waste brewer's yeast Saccharomyces cerevisiae was successfully used for the production of bioethanol using horsetail waste hydrolysate and corona pre-treated cotton material hydrolysate. Conclusion The obtained results showed that bacterial strain CKS7 has a significant, still unexplored enzymatic potential that could be used to achieve a cleaner, environmental friendly and economically acceptable biofuel production. [GRAPHICS] .", publisher = "Springer, Dordrecht", journal = "Waste and Biomass Valorization", title = "From Agricultural Waste to Biofuel: Enzymatic Potential of a Bacterial Isolate Streptomyces fulvissimus CKS7 for Bioethanol Production", pages = "174-165", number = "1", volume = "12", doi = "10.1007/s12649-020-00960-3" }
Mihajlovski, K., Buntić, A., Milić, M., Rajilić-Stojanović, M.,& Dimitrijević-Branković, S.. (2021). From Agricultural Waste to Biofuel: Enzymatic Potential of a Bacterial Isolate Streptomyces fulvissimus CKS7 for Bioethanol Production. in Waste and Biomass Valorization Springer, Dordrecht., 12(1), 165-174. https://doi.org/10.1007/s12649-020-00960-3
Mihajlovski K, Buntić A, Milić M, Rajilić-Stojanović M, Dimitrijević-Branković S. From Agricultural Waste to Biofuel: Enzymatic Potential of a Bacterial Isolate Streptomyces fulvissimus CKS7 for Bioethanol Production. in Waste and Biomass Valorization. 2021;12(1):165-174. doi:10.1007/s12649-020-00960-3 .
Mihajlovski, Katarina, Buntić, Aneta, Milić, Marija, Rajilić-Stojanović, Mirjana, Dimitrijević-Branković, Suzana, "From Agricultural Waste to Biofuel: Enzymatic Potential of a Bacterial Isolate Streptomyces fulvissimus CKS7 for Bioethanol Production" in Waste and Biomass Valorization, 12, no. 1 (2021):165-174, https://doi.org/10.1007/s12649-020-00960-3 . .