The aim of this study was to investigate the inhibitory activity of Gentiana lutea extracts on the enzyme myeloperoxidase (MPO), as well as the antioxidant activity of these extracts and their correlation with the total polyphenol content. Extracts were prepared using methanol (100%), water and ethanol aqueous solutions (96, 75, 50 and 25% v/v) as solvents for extraction. Also, isovitexin, amarogentin and gentiopicroside, pharmacologically active constituents of G. lutea were tested as potential inhibitors of MPO. Antioxidant activity of extracts was determined using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging test and also using cyclic voltammetry (CV). Among all extracts, the antioxidant capacity of 50% ethanol aqueous extract was the highest, both when measured using the DPPH test, with IC50 = 20.6 mu g/ml, and when using CV. Also, 50% ethanol extract, showed the best inhibition of MPO activity in comparison with other extracts. In the group of the selected G. lutea constitu...ents, gentiopicroside has proved to be the strongest inhibitor of MPO, with IC50 = 0.8 mu g/ml. Also, the concentration of G. lutea constituents were determined in all extracts, using Ultra Performance Liquid Chromatography (UPLC).
TY - JOUR
AU - Nastasijević, Branislav J.
AU - Lazarević-Pašti, Tamara
AU - Dimitrijević-Branković, Suzana
AU - Pašti, Igor A.
AU - Vujačić, Ana V.
AU - Joksić, Gordana
AU - Vasić, Vesna M.
PY - 2012
UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2130
AB - The aim of this study was to investigate the inhibitory activity of Gentiana lutea extracts on the enzyme myeloperoxidase (MPO), as well as the antioxidant activity of these extracts and their correlation with the total polyphenol content. Extracts were prepared using methanol (100%), water and ethanol aqueous solutions (96, 75, 50 and 25% v/v) as solvents for extraction. Also, isovitexin, amarogentin and gentiopicroside, pharmacologically active constituents of G. lutea were tested as potential inhibitors of MPO. Antioxidant activity of extracts was determined using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging test and also using cyclic voltammetry (CV). Among all extracts, the antioxidant capacity of 50% ethanol aqueous extract was the highest, both when measured using the DPPH test, with IC50 = 20.6 mu g/ml, and when using CV. Also, 50% ethanol extract, showed the best inhibition of MPO activity in comparison with other extracts. In the group of the selected G. lutea constituents, gentiopicroside has proved to be the strongest inhibitor of MPO, with IC50 = 0.8 mu g/ml. Also, the concentration of G. lutea constituents were determined in all extracts, using Ultra Performance Liquid Chromatography (UPLC).
PB - Elsevier, Amsterdam
T2 - Journal of Pharmaceutical and Biomedical Analysis
T1 - Inhibition of myeloperoxidase and antioxidative activity of Gentiana lutea extracts
EP - 196
SP - 191
VL - 66
DO - 10.1016/j.jpba.2012.03.052
ER -
@article{
author = "Nastasijević, Branislav J. and Lazarević-Pašti, Tamara and Dimitrijević-Branković, Suzana and Pašti, Igor A. and Vujačić, Ana V. and Joksić, Gordana and Vasić, Vesna M.",
year = "2012",
abstract = "The aim of this study was to investigate the inhibitory activity of Gentiana lutea extracts on the enzyme myeloperoxidase (MPO), as well as the antioxidant activity of these extracts and their correlation with the total polyphenol content. Extracts were prepared using methanol (100%), water and ethanol aqueous solutions (96, 75, 50 and 25% v/v) as solvents for extraction. Also, isovitexin, amarogentin and gentiopicroside, pharmacologically active constituents of G. lutea were tested as potential inhibitors of MPO. Antioxidant activity of extracts was determined using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging test and also using cyclic voltammetry (CV). Among all extracts, the antioxidant capacity of 50% ethanol aqueous extract was the highest, both when measured using the DPPH test, with IC50 = 20.6 mu g/ml, and when using CV. Also, 50% ethanol extract, showed the best inhibition of MPO activity in comparison with other extracts. In the group of the selected G. lutea constituents, gentiopicroside has proved to be the strongest inhibitor of MPO, with IC50 = 0.8 mu g/ml. Also, the concentration of G. lutea constituents were determined in all extracts, using Ultra Performance Liquid Chromatography (UPLC).",
publisher = "Elsevier, Amsterdam",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
title = "Inhibition of myeloperoxidase and antioxidative activity of Gentiana lutea extracts",
pages = "196-191",
volume = "66",
doi = "10.1016/j.jpba.2012.03.052"
}
Nastasijević, B. J., Lazarević-Pašti, T., Dimitrijević-Branković, S., Pašti, I. A., Vujačić, A. V., Joksić, G.,& Vasić, V. M.. (2012). Inhibition of myeloperoxidase and antioxidative activity of Gentiana lutea extracts. in Journal of Pharmaceutical and Biomedical Analysis
Elsevier, Amsterdam., 66, 191-196.
https://doi.org/10.1016/j.jpba.2012.03.052
Nastasijević BJ, Lazarević-Pašti T, Dimitrijević-Branković S, Pašti IA, Vujačić AV, Joksić G, Vasić VM. Inhibition of myeloperoxidase and antioxidative activity of Gentiana lutea extracts. in Journal of Pharmaceutical and Biomedical Analysis. 2012;66:191-196.
doi:10.1016/j.jpba.2012.03.052 .
Nastasijević, Branislav J., Lazarević-Pašti, Tamara, Dimitrijević-Branković, Suzana, Pašti, Igor A., Vujačić, Ana V., Joksić, Gordana, Vasić, Vesna M., "Inhibition of myeloperoxidase and antioxidative activity of Gentiana lutea extracts" in Journal of Pharmaceutical and Biomedical Analysis, 66 (2012):191-196,
https://doi.org/10.1016/j.jpba.2012.03.052 . .
