Efficient immobilization of lipase from Candida rugosa by entrapment into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels under mild conditions
Samo za registrovane korisnike
2012
Autori
Milašinović, NikolaMilosavljević, Nedeljko
Filipović, Jovanka
Knežević-Jugović, Zorica
Kalagasidis Krušić, Melina
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Temperature and pH-sensitive hydrogels, based on N-isopropylacrylamide and itaconic acid, with varying comonomer ratios and crosslinking agent content, were prepared by free radical crosslinking copolymerization. The immobilization of lipase from Candida rugosa was carried out by post-loading entrapment method at different temperatures until equilibrium swelling was achieved. The effects of the hydrogel composition and the immobilization temperature on the hydrogel-binding capacity, immobilized lipase specific activity, as well as the enzyme leakage were studied. It was found that the NiPAAm/IA ratio, crosslinking agent concentration and the temperature at which the entrapment was performed significantly affected the hydrogel-binding capacity. The biocatalysts obtained by entrapment into hydrogel with the highest itaconic acid content at 5 A degrees C exhibited both highest binding capacity and the highest specific activity, but appeared to be less suitable for repeated uses than those... obtained at 25 A degrees C.
Ključne reči:
Lipase from Candida rugosa / Immobilization temperature / Hydrogel / N-isopropylacrylamide / Itaconic acidIzvor:
Polymer Bulletin, 2012, 69, 3, 347-361Izdavač:
- Springer, New York
Finansiranje / projekti:
- Razvoj novih inkapsulacionih i enzimskih tehnologija za proizvodnju biokatalizatora i biološki aktivnih komponenata hrane u cilju povećanja njene konkurentnosti, kvaliteta i bezbednosti (RS-46010)
- Sinteza i karakterizacija novih funkcionalnih polimera i polimernih nanokompozita (RS-172062)
DOI: 10.1007/s00289-012-0737-7
ISSN: 0170-0839
WoS: 000305227500008
Scopus: 2-s2.0-84864616485
Institucija/grupa
Tehnološko-metalurški fakultetTY - JOUR AU - Milašinović, Nikola AU - Milosavljević, Nedeljko AU - Filipović, Jovanka AU - Knežević-Jugović, Zorica AU - Kalagasidis Krušić, Melina PY - 2012 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2132 AB - Temperature and pH-sensitive hydrogels, based on N-isopropylacrylamide and itaconic acid, with varying comonomer ratios and crosslinking agent content, were prepared by free radical crosslinking copolymerization. The immobilization of lipase from Candida rugosa was carried out by post-loading entrapment method at different temperatures until equilibrium swelling was achieved. The effects of the hydrogel composition and the immobilization temperature on the hydrogel-binding capacity, immobilized lipase specific activity, as well as the enzyme leakage were studied. It was found that the NiPAAm/IA ratio, crosslinking agent concentration and the temperature at which the entrapment was performed significantly affected the hydrogel-binding capacity. The biocatalysts obtained by entrapment into hydrogel with the highest itaconic acid content at 5 A degrees C exhibited both highest binding capacity and the highest specific activity, but appeared to be less suitable for repeated uses than those obtained at 25 A degrees C. PB - Springer, New York T2 - Polymer Bulletin T1 - Efficient immobilization of lipase from Candida rugosa by entrapment into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels under mild conditions EP - 361 IS - 3 SP - 347 VL - 69 DO - 10.1007/s00289-012-0737-7 ER -
@article{ author = "Milašinović, Nikola and Milosavljević, Nedeljko and Filipović, Jovanka and Knežević-Jugović, Zorica and Kalagasidis Krušić, Melina", year = "2012", abstract = "Temperature and pH-sensitive hydrogels, based on N-isopropylacrylamide and itaconic acid, with varying comonomer ratios and crosslinking agent content, were prepared by free radical crosslinking copolymerization. The immobilization of lipase from Candida rugosa was carried out by post-loading entrapment method at different temperatures until equilibrium swelling was achieved. The effects of the hydrogel composition and the immobilization temperature on the hydrogel-binding capacity, immobilized lipase specific activity, as well as the enzyme leakage were studied. It was found that the NiPAAm/IA ratio, crosslinking agent concentration and the temperature at which the entrapment was performed significantly affected the hydrogel-binding capacity. The biocatalysts obtained by entrapment into hydrogel with the highest itaconic acid content at 5 A degrees C exhibited both highest binding capacity and the highest specific activity, but appeared to be less suitable for repeated uses than those obtained at 25 A degrees C.", publisher = "Springer, New York", journal = "Polymer Bulletin", title = "Efficient immobilization of lipase from Candida rugosa by entrapment into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels under mild conditions", pages = "361-347", number = "3", volume = "69", doi = "10.1007/s00289-012-0737-7" }
Milašinović, N., Milosavljević, N., Filipović, J., Knežević-Jugović, Z.,& Kalagasidis Krušić, M.. (2012). Efficient immobilization of lipase from Candida rugosa by entrapment into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels under mild conditions. in Polymer Bulletin Springer, New York., 69(3), 347-361. https://doi.org/10.1007/s00289-012-0737-7
Milašinović N, Milosavljević N, Filipović J, Knežević-Jugović Z, Kalagasidis Krušić M. Efficient immobilization of lipase from Candida rugosa by entrapment into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels under mild conditions. in Polymer Bulletin. 2012;69(3):347-361. doi:10.1007/s00289-012-0737-7 .
Milašinović, Nikola, Milosavljević, Nedeljko, Filipović, Jovanka, Knežević-Jugović, Zorica, Kalagasidis Krušić, Melina, "Efficient immobilization of lipase from Candida rugosa by entrapment into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels under mild conditions" in Polymer Bulletin, 69, no. 3 (2012):347-361, https://doi.org/10.1007/s00289-012-0737-7 . .