Controlled release of lipase from Candida rugosa loaded into hydrogels of N-isopropylacrylamide and itaconic acid
Authorized Users Only
2012
Authors
Milašinović, Nikola
Knežević-Jugović, Zorica

Milosavljević, Nedeljko
Filipović, Jovanka
Kalagasidis Krušić, Melina

Article (Published version)

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The series of poly(N-isopropylacrylamide-co-itaconic acid) hydrogels, with lipase from Candida rugosa as a model protein, were synthesized by free radical copolymerization. The composition of hydrogels was varied by monomers ratio, crosslinking agent concentration and amounts of lipase, which was loaded by in situ polymerization. All samples were characterized regarding morphology. The investigation of hydrogel swelling properties revealed their pH and temperature sensitive character. Protein loading efficiency, release profiles and the specific activity yield of the released lipase were also investigated as a function of hydrogel composition, protein content and pH, at the physiological temperature of 37 degrees C. Copolymers of N-isopropylacrylamide and itaconic acid presented high lipase loading efficiency. Another very important feature of these copolymers was that the protein release kinetic strongly depended on the pH value of the medium. The diffusion exponents values around 1 d...enoted that these hydrogel compositions could be adjusted to follow near zero-order kinetics. Namely, hydrogel formulations released low amounts of lipase at pH 2.20, but much higher released protein quantities were observed at pH 6.80 enabling these copolymers to be attractive candidates as site specific protein oral drug delivery systems.
Keywords:
Hydrogels / N-isopropylacrylamide / Itaconic acid / Candida rugosa lipase / Controlled releaseSource:
International Journal of Pharmaceutics, 2012, 436, 1-2, 332-340Publisher:
- Elsevier Science Bv, Amsterdam
Funding / projects:
- EUREKA E!6750, 172062
DOI: 10.1016/j.ijpharm.2012.06.050
ISSN: 0378-5173
PubMed: 22759642
WoS: 000308597600038
Scopus: 2-s2.0-84865307480
Institution/Community
Tehnološko-metalurški fakultetTY - JOUR AU - Milašinović, Nikola AU - Knežević-Jugović, Zorica AU - Milosavljević, Nedeljko AU - Filipović, Jovanka AU - Kalagasidis Krušić, Melina PY - 2012 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2170 AB - The series of poly(N-isopropylacrylamide-co-itaconic acid) hydrogels, with lipase from Candida rugosa as a model protein, were synthesized by free radical copolymerization. The composition of hydrogels was varied by monomers ratio, crosslinking agent concentration and amounts of lipase, which was loaded by in situ polymerization. All samples were characterized regarding morphology. The investigation of hydrogel swelling properties revealed their pH and temperature sensitive character. Protein loading efficiency, release profiles and the specific activity yield of the released lipase were also investigated as a function of hydrogel composition, protein content and pH, at the physiological temperature of 37 degrees C. Copolymers of N-isopropylacrylamide and itaconic acid presented high lipase loading efficiency. Another very important feature of these copolymers was that the protein release kinetic strongly depended on the pH value of the medium. The diffusion exponents values around 1 denoted that these hydrogel compositions could be adjusted to follow near zero-order kinetics. Namely, hydrogel formulations released low amounts of lipase at pH 2.20, but much higher released protein quantities were observed at pH 6.80 enabling these copolymers to be attractive candidates as site specific protein oral drug delivery systems. PB - Elsevier Science Bv, Amsterdam T2 - International Journal of Pharmaceutics T1 - Controlled release of lipase from Candida rugosa loaded into hydrogels of N-isopropylacrylamide and itaconic acid EP - 340 IS - 1-2 SP - 332 VL - 436 DO - 10.1016/j.ijpharm.2012.06.050 ER -
@article{ author = "Milašinović, Nikola and Knežević-Jugović, Zorica and Milosavljević, Nedeljko and Filipović, Jovanka and Kalagasidis Krušić, Melina", year = "2012", abstract = "The series of poly(N-isopropylacrylamide-co-itaconic acid) hydrogels, with lipase from Candida rugosa as a model protein, were synthesized by free radical copolymerization. The composition of hydrogels was varied by monomers ratio, crosslinking agent concentration and amounts of lipase, which was loaded by in situ polymerization. All samples were characterized regarding morphology. The investigation of hydrogel swelling properties revealed their pH and temperature sensitive character. Protein loading efficiency, release profiles and the specific activity yield of the released lipase were also investigated as a function of hydrogel composition, protein content and pH, at the physiological temperature of 37 degrees C. Copolymers of N-isopropylacrylamide and itaconic acid presented high lipase loading efficiency. Another very important feature of these copolymers was that the protein release kinetic strongly depended on the pH value of the medium. The diffusion exponents values around 1 denoted that these hydrogel compositions could be adjusted to follow near zero-order kinetics. Namely, hydrogel formulations released low amounts of lipase at pH 2.20, but much higher released protein quantities were observed at pH 6.80 enabling these copolymers to be attractive candidates as site specific protein oral drug delivery systems.", publisher = "Elsevier Science Bv, Amsterdam", journal = "International Journal of Pharmaceutics", title = "Controlled release of lipase from Candida rugosa loaded into hydrogels of N-isopropylacrylamide and itaconic acid", pages = "340-332", number = "1-2", volume = "436", doi = "10.1016/j.ijpharm.2012.06.050" }
Milašinović, N., Knežević-Jugović, Z., Milosavljević, N., Filipović, J.,& Kalagasidis Krušić, M.. (2012). Controlled release of lipase from Candida rugosa loaded into hydrogels of N-isopropylacrylamide and itaconic acid. in International Journal of Pharmaceutics Elsevier Science Bv, Amsterdam., 436(1-2), 332-340. https://doi.org/10.1016/j.ijpharm.2012.06.050
Milašinović N, Knežević-Jugović Z, Milosavljević N, Filipović J, Kalagasidis Krušić M. Controlled release of lipase from Candida rugosa loaded into hydrogels of N-isopropylacrylamide and itaconic acid. in International Journal of Pharmaceutics. 2012;436(1-2):332-340. doi:10.1016/j.ijpharm.2012.06.050 .
Milašinović, Nikola, Knežević-Jugović, Zorica, Milosavljević, Nedeljko, Filipović, Jovanka, Kalagasidis Krušić, Melina, "Controlled release of lipase from Candida rugosa loaded into hydrogels of N-isopropylacrylamide and itaconic acid" in International Journal of Pharmaceutics, 436, no. 1-2 (2012):332-340, https://doi.org/10.1016/j.ijpharm.2012.06.050 . .