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One-step, inexpensive high yield strategy for Candida antarctica lipase A isolation using hydroxyapatite
dc.creator | Dimitrijević, Aleksandra | |
dc.creator | Veličković, Dušan | |
dc.creator | Bihelović, Filip | |
dc.creator | Bezbradica, Dejan | |
dc.creator | Jankov, Ratko | |
dc.creator | Milosavić, Nenad | |
dc.date.accessioned | 2021-03-10T11:54:57Z | |
dc.date.available | 2021-03-10T11:54:57Z | |
dc.date.issued | 2012 | |
dc.identifier.issn | 0960-8524 | |
dc.identifier.uri | http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2201 | |
dc.description.abstract | Lipase A from Candida antarctica (CAL A) was purified to apparent homogeneity in a single step using hydroxyapatite (HAP) chromatography. CAL A bound to HAP was eluted with 10 mM Na-phosphate buffer, pH 7.0 containing 0.5% Triton X-100. The protocol resulted in a 3.74-fold purification with 94.7% final recovery and 400.83 U/mg specific activity. Silver staining after SDS-PAGE revealed the presence a single band of 45 kDa. The enzyme exhibited a temperature optimum of 60 degrees C, was unaffected by monovalent metal ions, but was destabilized by divalent metal ions (Zn2+, Ca2+, Mg2+, Cu2+, Mn2+) and stimulated by 50 mM Fe2+. Detergents at 0.1% concentrations did not affect lipase activity. Except for Triton X-100, detergent concentrations of 1% had a destabilizing effect. | en |
dc.publisher | Elsevier Sci Ltd, Oxford | |
dc.relation | info:eu-repo/grantAgreement/EC/FP7/256716/EU// | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172049/RS// | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46010/RS// | |
dc.rights | restrictedAccess | |
dc.source | Bioresource Technology | |
dc.subject | Candida antarctica | en |
dc.subject | Lipase | en |
dc.subject | Isolation | en |
dc.subject | Purification | en |
dc.subject | Hydroxyapatite | en |
dc.title | One-step, inexpensive high yield strategy for Candida antarctica lipase A isolation using hydroxyapatite | en |
dc.type | article | |
dc.rights.license | ARR | |
dc.citation.epage | 362 | |
dc.citation.other | 107: 358-362 | |
dc.citation.rank | aM21 | |
dc.citation.spage | 358 | |
dc.citation.volume | 107 | |
dc.identifier.doi | 10.1016/j.biortech.2011.11.077 | |
dc.identifier.pmid | 22209131 | |
dc.identifier.scopus | 2-s2.0-84856573973 | |
dc.identifier.wos | 000301620600050 | |
dc.type.version | publishedVersion |