Adding Value to the Oil Cake as a Waste from Oil Processing Industry: Production of Lipase and Protease by Candida utilis in Solid State Fermentation
Само за регистроване кориснике
2012
Аутори
Moftah, Omar A.S.Grbavčić, Sanja
Žuža, Milena
Luković, Nevena
Bezbradica, Dejan
Knežević-Jugović, Zorica
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Olive oil cake is a by-product from the olive oil processing industry and can be used for the lipase and protease production by Candida utilis in solid state fermentation. Different carbon and nitrogen sources were evaluated, and the results showed that the supplementation of the substrate with maltose and starch as carbon sources and yeast extract as a nitrogen source significantly increased the lipase production. The best results were obtained with maltose, whereas rather low lipase and protease activities were found with glucose and oleic acid. Response surface methodology and a five-level-three-factor central composite rotatable design were used to evaluate the effects of the initial moisture content, inoculum size and fermentation time on both lipase and protease activity levels. A lipase activity value of approximate to 25 Ug(-1) and a protease activity value of 110 Ug(-1) were obtained under the optimized fermentation conditions. An alkaline treatment of the substrate appeared t...o be efficient, leading to increases of 39% and 133% in the lipase and protease production, respectively. The results showed that the olive cake could be a good source for enzyme production by solid state fermentation.
Кључне речи:
Olive oil cake / Candida utilis / Lipase / Protease / Optimization / Solid state fermentationИзвор:
Applied Biochemistry and Biotechnology, 2012, 166, 2, 348-364Издавач:
- Springer, New York
Финансирање / пројекти:
- Развој нових инкапсулационих и ензимских технологија за производњу биокатализатора и биолошки активних компонената хране у циљу повећања њене конкурентности, квалитета и безбедности (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46010)
DOI: 10.1007/s12010-011-9429-2
ISSN: 0273-2289
PubMed: 22081325
WoS: 000300296000009
Scopus: 2-s2.0-84856255593
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Moftah, Omar A.S. AU - Grbavčić, Sanja AU - Žuža, Milena AU - Luković, Nevena AU - Bezbradica, Dejan AU - Knežević-Jugović, Zorica PY - 2012 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2227 AB - Olive oil cake is a by-product from the olive oil processing industry and can be used for the lipase and protease production by Candida utilis in solid state fermentation. Different carbon and nitrogen sources were evaluated, and the results showed that the supplementation of the substrate with maltose and starch as carbon sources and yeast extract as a nitrogen source significantly increased the lipase production. The best results were obtained with maltose, whereas rather low lipase and protease activities were found with glucose and oleic acid. Response surface methodology and a five-level-three-factor central composite rotatable design were used to evaluate the effects of the initial moisture content, inoculum size and fermentation time on both lipase and protease activity levels. A lipase activity value of approximate to 25 Ug(-1) and a protease activity value of 110 Ug(-1) were obtained under the optimized fermentation conditions. An alkaline treatment of the substrate appeared to be efficient, leading to increases of 39% and 133% in the lipase and protease production, respectively. The results showed that the olive cake could be a good source for enzyme production by solid state fermentation. PB - Springer, New York T2 - Applied Biochemistry and Biotechnology T1 - Adding Value to the Oil Cake as a Waste from Oil Processing Industry: Production of Lipase and Protease by Candida utilis in Solid State Fermentation EP - 364 IS - 2 SP - 348 VL - 166 DO - 10.1007/s12010-011-9429-2 ER -
@article{ author = "Moftah, Omar A.S. and Grbavčić, Sanja and Žuža, Milena and Luković, Nevena and Bezbradica, Dejan and Knežević-Jugović, Zorica", year = "2012", abstract = "Olive oil cake is a by-product from the olive oil processing industry and can be used for the lipase and protease production by Candida utilis in solid state fermentation. Different carbon and nitrogen sources were evaluated, and the results showed that the supplementation of the substrate with maltose and starch as carbon sources and yeast extract as a nitrogen source significantly increased the lipase production. The best results were obtained with maltose, whereas rather low lipase and protease activities were found with glucose and oleic acid. Response surface methodology and a five-level-three-factor central composite rotatable design were used to evaluate the effects of the initial moisture content, inoculum size and fermentation time on both lipase and protease activity levels. A lipase activity value of approximate to 25 Ug(-1) and a protease activity value of 110 Ug(-1) were obtained under the optimized fermentation conditions. An alkaline treatment of the substrate appeared to be efficient, leading to increases of 39% and 133% in the lipase and protease production, respectively. The results showed that the olive cake could be a good source for enzyme production by solid state fermentation.", publisher = "Springer, New York", journal = "Applied Biochemistry and Biotechnology", title = "Adding Value to the Oil Cake as a Waste from Oil Processing Industry: Production of Lipase and Protease by Candida utilis in Solid State Fermentation", pages = "364-348", number = "2", volume = "166", doi = "10.1007/s12010-011-9429-2" }
Moftah, O. A.S., Grbavčić, S., Žuža, M., Luković, N., Bezbradica, D.,& Knežević-Jugović, Z.. (2012). Adding Value to the Oil Cake as a Waste from Oil Processing Industry: Production of Lipase and Protease by Candida utilis in Solid State Fermentation. in Applied Biochemistry and Biotechnology Springer, New York., 166(2), 348-364. https://doi.org/10.1007/s12010-011-9429-2
Moftah OA, Grbavčić S, Žuža M, Luković N, Bezbradica D, Knežević-Jugović Z. Adding Value to the Oil Cake as a Waste from Oil Processing Industry: Production of Lipase and Protease by Candida utilis in Solid State Fermentation. in Applied Biochemistry and Biotechnology. 2012;166(2):348-364. doi:10.1007/s12010-011-9429-2 .
Moftah, Omar A.S., Grbavčić, Sanja, Žuža, Milena, Luković, Nevena, Bezbradica, Dejan, Knežević-Jugović, Zorica, "Adding Value to the Oil Cake as a Waste from Oil Processing Industry: Production of Lipase and Protease by Candida utilis in Solid State Fermentation" in Applied Biochemistry and Biotechnology, 166, no. 2 (2012):348-364, https://doi.org/10.1007/s12010-011-9429-2 . .