Show simple item record

dc.creatorMilašinović, Nikola
dc.creatorKnežević-Jugović, Zorica
dc.creatorJakovljević, Živana
dc.creatorFilipović, Jovanka
dc.creatorKalagasidis-Krušić, Melina
dc.date.accessioned2021-03-10T11:57:05Z
dc.date.available2021-03-10T11:57:05Z
dc.date.issued2012
dc.identifier.issn1385-8947
dc.identifier.urihttp://TechnoRep.tmf.bg.ac.rs/handle/123456789/2235
dc.description.abstractA commercial Candida rugosa lipase was immobilized into poly(N-isopropylacrylamide-co-itaconic acid) hydrogel under different pH immobilization conditions, characterized and evaluated for both hydrolytic activity in an aqueous medium and esterolytic activity in an organic medium. Although the apparent enzyme activity in the hydrolytic reaction was acceptable, the immobilized lipase appeared to be more suitable for application in ester synthesis in low aqueous system based on n-hexane. The esterification reaction parameters, such as temperature, biocatalyst amount, added water content and substrate concentration was evaluated and optimized by response surface analysis. An adequate statistical quadratic model was obtained, making it possible to predict ester yields from known values of the four main factors. Under optimized conditions, the yield of ester gt 90% could be achieved after 48 h of reaction, corresponding to the volumetric activity of 4.75 mmol L-1 h(-1). The operational stability of the immobilized system in esterification reaction proved to be highly attractive with 15 consecutive 48 h uses with a residual activity of 67.4%, implying that the developed hydrogel and immobilized system could provide a promising solution for the flavor ester synthesis at the industrial scale.en
dc.publisherElsevier Science Sa, Lausanne
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46010/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172062/RS//
dc.rightsrestrictedAccess
dc.sourceChemical Engineering Journal
dc.subjectN-Isopropylacrylamideen
dc.subjectItaconic aciden
dc.subjectEnzyme immobilizationen
dc.subjectCandida rugosa lipaseen
dc.subjectEster synthesisen
dc.titleSynthesis of n-amyl isobutyrate catalyzed by Candida rugosa lipase immobilized into poly(N-isopropylacrylamide-co-itaconic acid) hydrogelsen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage623
dc.citation.other181: 614-623
dc.citation.rankaM21
dc.citation.spage614
dc.citation.volume181
dc.identifier.doi10.1016/j.cej.2011.11.115
dc.identifier.rcubconv_3768
dc.identifier.scopus2-s2.0-84856318182
dc.identifier.wos000301087000069
dc.type.versionpublishedVersion


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record