Prikaz osnovnih podataka o dokumentu

dc.creatorSeratlić, Sanja
dc.creatorBugarski, Branko
dc.creatorRadulović, Zorica
dc.creatorDejmek, Petr
dc.creatorWadso, Lars
dc.creatorNedović, Viktor
dc.date.accessioned2021-03-10T11:59:04Z
dc.date.available2021-03-10T11:59:04Z
dc.date.issued2013
dc.identifier.issn1330-9862
dc.identifier.urihttp://TechnoRep.tmf.bg.ac.rs/handle/123456789/2267
dc.description.abstractThe exposure of bacterial cells to pulsed electric fields (PEF) leads to the reversible formation of pores in the cell membrane if an applied energy is below the critical level. Therefore, the effect of electric field pulses with amplitudes below 14 kV/cm and the applied energy up to 12.2 J/cm(3) on the growth of Lactobacillus plantarum 564 cells was investigated. After PEF treatments, the growth of lactobacilli in De Man-Rogosa-Sharpe broth at 37 degrees C was monitored by isothermal calorimetry, absorbance and plate counts. All the applied treatments resulted in a higher growth rate of PEF-treated cells during early and mid-log phase, especially bacterial samples treated with lower field intensities (1.3-5.5 J/cm(3)). The transport of ions and molecules through the cell membrane (which facilitates the growth of electroporated lactobacilli) was particularly evident in the mid-exponential growth phase, where the doubling time was reduced more than 3 times after the exposure to electric pulses of 5.5 Yore. The heat production rate during the growth of electroporated cells was also higher, indicating the enhanced metabolic activity of PEF-treated cells. Moreover, the electroporated cells had a better acidification ability than the untreated ones. It can be summarized that the applied PEF treatments with an energy input of below 12 J/cm(3) potentially induce reversible electroporation of the cell membrane, which has a positive impact on the growth and metabolic activity of the cells of lactobacilli.en
dc.publisherUniversity of Zagreb
dc.relationBASI-LEUS
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46010/RS//
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceFood Technology and Biotechnology
dc.subjectLactobacillus plantarumen
dc.subjectpulsed electric fieldsen
dc.subjectisothermal calorimetryen
dc.titleElectroporation Enhances the Metabolic Activity of Lactobacillus plantarum 564en
dc.typearticle
dc.rights.licenseBY
dc.citation.epage452
dc.citation.issue4
dc.citation.other51(4): 446-452
dc.citation.rankM22
dc.citation.spage446
dc.citation.volume51
dc.identifier.fulltexthttp://TechnoRep.tmf.bg.ac.rs/bitstream/id/11621/FTB_51-4_446-452.pdf
dc.identifier.pmid
dc.identifier.rcubhttps://hdl.handle.net/21.15107/rcub_technorep_2267
dc.identifier.scopus2-s2.0-84902368913
dc.identifier.wos000329768300002
dc.type.versionpublishedVersion


Dokumenti

Thumbnail

Ovaj dokument se pojavljuje u sledećim kolekcijama

Prikaz osnovnih podataka o dokumentu