Integrated production of lactic acid and biomass on distillery stillage
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The possibilities of parallel lactic acid and biomass production in batch and fed-batch fermentation on distillery stillage from bioethanol production were studied. The highest lactic acid yield and productivity of 92.3 % and 1.49 g L-1 h(-1) were achieved in batch fermentation with initial sugar concentration of 55 g L-1. A significant improvement of the process was achieved in fed-batch fermentation where the concentration of lactic acid was increased to 47.6 % and volumetric productivity for 21 % over the batch process. A high number of Lactobacillus rhamnosus ATCC 7469 viable cells of 10(9) CFU ml(-1) was attained at the end of fed-batch fermentation. The survival of 92.9 % of L. rhamnosus cells after 3 h of incubation at pH 2.5 validated that the fermentation media remained after lactic acid removal could be used as a biomass-enriched animal feed thus making an additional value to the process.
Keywords:Lactic acid / Lactobacillus rhamnosus / Batch fermentation / Fed-batch fermentation / Distillery stillage / Probiotic biomass
Source:Bioprocess and Biosystems Engineering, 2013, 36, 9, 1157-1164
- Springer, New York
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