Antioxidant effects of twelve 3-substituted-5,5-diphenylhydantoins in concentrations of 0.01, 0.1, 1, 10, and 100 mu M on human colon cancer cell line HCT-116 were determined 24 h after treatment. The concentrations of superoxide anion radical (O(2)radical anion), nitrites (NO2-), and total glutathione were determined spectrophotometrically. Results indicated that treatment with all compounds induced a decrease in O(2)radical anion and an increase in NO(2)(-)concentrations in HCT-116 cells. Treatment with all compounds resulted in an increase in glutathione, and this indicates changes in redox homeostasis. Based on the obtained results, the investigated hydantoins act as antioxidants because they decrease the production of superoxide anion radical and increase concentrations of glutathione, but they also induce an increase in nitrite production.
TY - JOUR
AU - Obradović, Ana
AU - Zizić, Jovana
AU - Trišović, Nemanja
AU - Božić, Bojan
AU - Ušćumlić, Gordana
AU - Božić, Biljana
AU - Marković, Snežana
PY - 2013
UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2427
AB - Antioxidant effects of twelve 3-substituted-5,5-diphenylhydantoins in concentrations of 0.01, 0.1, 1, 10, and 100 mu M on human colon cancer cell line HCT-116 were determined 24 h after treatment. The concentrations of superoxide anion radical (O(2)radical anion), nitrites (NO2-), and total glutathione were determined spectrophotometrically. Results indicated that treatment with all compounds induced a decrease in O(2)radical anion and an increase in NO(2)(-)concentrations in HCT-116 cells. Treatment with all compounds resulted in an increase in glutathione, and this indicates changes in redox homeostasis. Based on the obtained results, the investigated hydantoins act as antioxidants because they decrease the production of superoxide anion radical and increase concentrations of glutathione, but they also induce an increase in nitrite production.
PB - Tubitak Scientific & Technical Research Council Turkey, Ankara
T2 - Turkish Journal of Biology
T1 - Evaluation of antioxidative effects of twelve 3-substituted-5,5-diphenylhydantoins on human colon cancer cell line HCT-116
EP - 747
IS - 6
SP - 741
VL - 37
DO - 10.3906/biy-1302-15
ER -
@article{
author = "Obradović, Ana and Zizić, Jovana and Trišović, Nemanja and Božić, Bojan and Ušćumlić, Gordana and Božić, Biljana and Marković, Snežana",
year = "2013",
abstract = "Antioxidant effects of twelve 3-substituted-5,5-diphenylhydantoins in concentrations of 0.01, 0.1, 1, 10, and 100 mu M on human colon cancer cell line HCT-116 were determined 24 h after treatment. The concentrations of superoxide anion radical (O(2)radical anion), nitrites (NO2-), and total glutathione were determined spectrophotometrically. Results indicated that treatment with all compounds induced a decrease in O(2)radical anion and an increase in NO(2)(-)concentrations in HCT-116 cells. Treatment with all compounds resulted in an increase in glutathione, and this indicates changes in redox homeostasis. Based on the obtained results, the investigated hydantoins act as antioxidants because they decrease the production of superoxide anion radical and increase concentrations of glutathione, but they also induce an increase in nitrite production.",
publisher = "Tubitak Scientific & Technical Research Council Turkey, Ankara",
journal = "Turkish Journal of Biology",
title = "Evaluation of antioxidative effects of twelve 3-substituted-5,5-diphenylhydantoins on human colon cancer cell line HCT-116",
pages = "747-741",
number = "6",
volume = "37",
doi = "10.3906/biy-1302-15"
}
Obradović, A., Zizić, J., Trišović, N., Božić, B., Ušćumlić, G., Božić, B.,& Marković, S.. (2013). Evaluation of antioxidative effects of twelve 3-substituted-5,5-diphenylhydantoins on human colon cancer cell line HCT-116. in Turkish Journal of Biology
Tubitak Scientific & Technical Research Council Turkey, Ankara., 37(6), 741-747.
https://doi.org/10.3906/biy-1302-15
Obradović A, Zizić J, Trišović N, Božić B, Ušćumlić G, Božić B, Marković S. Evaluation of antioxidative effects of twelve 3-substituted-5,5-diphenylhydantoins on human colon cancer cell line HCT-116. in Turkish Journal of Biology. 2013;37(6):741-747.
doi:10.3906/biy-1302-15 .
Obradović, Ana, Zizić, Jovana, Trišović, Nemanja, Božić, Bojan, Ušćumlić, Gordana, Božić, Biljana, Marković, Snežana, "Evaluation of antioxidative effects of twelve 3-substituted-5,5-diphenylhydantoins on human colon cancer cell line HCT-116" in Turkish Journal of Biology, 37, no. 6 (2013):741-747,
https://doi.org/10.3906/biy-1302-15 . .
