Prikaz osnovnih podataka o dokumentu

dc.creatorVunjak-Novaković, Gordana
dc.creatorMartin, Ivan
dc.creatorObradović, Bojana
dc.creatorTreppo, S
dc.creatorGrodzinsky, AJ
dc.creatorLanger, R
dc.creatorFreed, LE
dc.date.accessioned2021-03-10T09:48:22Z
dc.date.available2021-03-10T09:48:22Z
dc.date.issued1999
dc.identifier.issn0736-0266
dc.identifier.urihttp://TechnoRep.tmf.bg.ac.rs/handle/123456789/243
dc.description.abstractCartilaginous constructs have been grown in vitro with use of isolated cells, biodegradable polymer scaffolds, and bioreactors. In the present work, the relationships between the composition and mechanical properties of engineered cartilage constructs were studied by culturing bovine calf articular chondrocytes on fibrous polyglycolic acid scaffolds (5 mm in diameter, 2-mm thick, and 97% porous) in three different environments: static flasks, mixed flasks, and rotating vessels. After 6 weeks of cultivation, the composition, morphology, and mechanical function of the constructs in radially confined static and dynamic compression all depended on the conditions of in vitro cultivation. Static culture yielded small and fragile constructs, while turbulent flow in mixed flasks yielded constructs with fibrous outer capsules; both environments resulted in constructs with poor mechanical properties. The constructs that were cultured freely suspended in a dynamic laminar flow field in rotating vessels were the largest, contained continuous cartilage-like extracellular matrices with the highest fractions of glycosaminoglycan and collagen, and had the best mechanical properties. The equilibrium modulus, hydraulic permeability, dynamic stiffness, and streaming potential correlated with the wet-weight fractions of glycosaminoglycan, collagen, and water. These findings suggest that the hydrodynamic conditions in tissue-culture bioreactors can modulate the composition, morphology, mechanical properties, and electromechanical function of engineered cartilage.en
dc.publisherWiley, Hoboken
dc.relationNIAMS NIH HHSUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of Arthritis & Musculoskeletal & Skin Diseases (NIAMS) [AR33236] Funding Source: Medline
dc.relationNATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASESUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of Arthritis & Musculoskeletal & Skin Diseases (NIAMS) [R37AR033236, R0
dc.rightsopenAccess
dc.sourceJournal of Orthopaedic Research
dc.titleBioreactor cultivation conditions modulate the composition and mechanical properties of tissue-engineered cartilageen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage138
dc.citation.issue1
dc.citation.other17(1): 130-138
dc.citation.rankaM21
dc.citation.spage130
dc.citation.volume17
dc.identifier.doi10.1002/jor.1100170119
dc.identifier.fulltexthttp://TechnoRep.tmf.bg.ac.rs/bitstream/id/658/240.pdf
dc.identifier.pmid10073657
dc.identifier.scopus2-s2.0-0033043139
dc.identifier.wos000078670700019
dc.type.versionpublishedVersion


Dokumenti

Thumbnail

Ovaj dokument se pojavljuje u sledećim kolekcijama

Prikaz osnovnih podataka o dokumentu