Ethanol influenced fast microwave-assisted extraction for natural antioxidants obtaining from spent filter coffee
Abstract
Ethanol influenced microwave-assisted extraction was performed for obtaining of natural antioxidants from spent filter coffee. The process included response surface methodology which represented the significance of both investigated variables (ethanol concentration and time of microwave radiation) on all tested responses (extraction yield, total polyphenol content (TPC), DPPH radical inhibition activity and ferric reducing ability, FRAP). The highest TPC of 398.95 mg GAE/g of extract dry matter was obtained with 20% aqueous ethanol solution under just 40 s of microwave radiation (80 W), which implies that method was very effective and thereby saved time and chemicals. Obtained extracts showed great antioxidant activities in vitro. Even 20 mu g/mL of working extract concentration inhibited 90.47% of DPPH radicals. The results suggest that examined method provides fast obtaining of high values of bioactive compounds and also ensures their biological stability.
Keywords:
Spent coffee / Antioxidants / Microwave-assisted extraction / response surface methodology (RSM)Source:
Separation and Purification Technology, 2013, 118, 503-510Publisher:
- Elsevier, Amsterdam
Funding / projects:
- Application of biotechnological methods for sustainable exploitation of by-products of agro-industry (RS-31035)
DOI: 10.1016/j.seppur.2013.07.035
ISSN: 1383-5866
WoS: 000326994500060
Scopus: 2-s2.0-84883068266
Institution/Community
Tehnološko-metalurški fakultetTY - JOUR AU - Pavlović, Marija D. AU - Buntić, Aneta AU - Šiler-Marinković, Slavica AU - Dimitrijević-Branković, Suzana PY - 2013 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2443 AB - Ethanol influenced microwave-assisted extraction was performed for obtaining of natural antioxidants from spent filter coffee. The process included response surface methodology which represented the significance of both investigated variables (ethanol concentration and time of microwave radiation) on all tested responses (extraction yield, total polyphenol content (TPC), DPPH radical inhibition activity and ferric reducing ability, FRAP). The highest TPC of 398.95 mg GAE/g of extract dry matter was obtained with 20% aqueous ethanol solution under just 40 s of microwave radiation (80 W), which implies that method was very effective and thereby saved time and chemicals. Obtained extracts showed great antioxidant activities in vitro. Even 20 mu g/mL of working extract concentration inhibited 90.47% of DPPH radicals. The results suggest that examined method provides fast obtaining of high values of bioactive compounds and also ensures their biological stability. PB - Elsevier, Amsterdam T2 - Separation and Purification Technology T1 - Ethanol influenced fast microwave-assisted extraction for natural antioxidants obtaining from spent filter coffee EP - 510 SP - 503 VL - 118 DO - 10.1016/j.seppur.2013.07.035 ER -
@article{ author = "Pavlović, Marija D. and Buntić, Aneta and Šiler-Marinković, Slavica and Dimitrijević-Branković, Suzana", year = "2013", abstract = "Ethanol influenced microwave-assisted extraction was performed for obtaining of natural antioxidants from spent filter coffee. The process included response surface methodology which represented the significance of both investigated variables (ethanol concentration and time of microwave radiation) on all tested responses (extraction yield, total polyphenol content (TPC), DPPH radical inhibition activity and ferric reducing ability, FRAP). The highest TPC of 398.95 mg GAE/g of extract dry matter was obtained with 20% aqueous ethanol solution under just 40 s of microwave radiation (80 W), which implies that method was very effective and thereby saved time and chemicals. Obtained extracts showed great antioxidant activities in vitro. Even 20 mu g/mL of working extract concentration inhibited 90.47% of DPPH radicals. The results suggest that examined method provides fast obtaining of high values of bioactive compounds and also ensures their biological stability.", publisher = "Elsevier, Amsterdam", journal = "Separation and Purification Technology", title = "Ethanol influenced fast microwave-assisted extraction for natural antioxidants obtaining from spent filter coffee", pages = "510-503", volume = "118", doi = "10.1016/j.seppur.2013.07.035" }
Pavlović, M. D., Buntić, A., Šiler-Marinković, S.,& Dimitrijević-Branković, S.. (2013). Ethanol influenced fast microwave-assisted extraction for natural antioxidants obtaining from spent filter coffee. in Separation and Purification Technology Elsevier, Amsterdam., 118, 503-510. https://doi.org/10.1016/j.seppur.2013.07.035
Pavlović MD, Buntić A, Šiler-Marinković S, Dimitrijević-Branković S. Ethanol influenced fast microwave-assisted extraction for natural antioxidants obtaining from spent filter coffee. in Separation and Purification Technology. 2013;118:503-510. doi:10.1016/j.seppur.2013.07.035 .
Pavlović, Marija D., Buntić, Aneta, Šiler-Marinković, Slavica, Dimitrijević-Branković, Suzana, "Ethanol influenced fast microwave-assisted extraction for natural antioxidants obtaining from spent filter coffee" in Separation and Purification Technology, 118 (2013):503-510, https://doi.org/10.1016/j.seppur.2013.07.035 . .