Adsorption of lipase from Candida rugosa on multi walled carbon nanotubes

In this work lipase from Candida rugosa was adsorbed on unmodified surface of multi walled carbon nanotubes (raw-MWCIsIT). The effects of immobilization time, initial enzyme concentration and buffer ionic strength on enzyme loading and activity of immobilized preparations were tested. High loadings are attained. The immobilized enzyme obtained at lowest initial enzyme concentration and high ionic strength retained 85% of initial enzyme activity. It is assumed that immobilization on hydrophobic surface led to conformational changes that resulted in the adsorption of lipase in active conformation. Immobilized preparations were characterized, with FT-IR spectroscopy, AFM, and cyclic voltammetry.

Keywords:

Lipase / Carbon nanotubes / Adsorption / FT-IR spectroscopy / AFM / Cyclic voltammetry

Source:
Journal of Industrial and Engineering Chemistry, 2013, 19, 1, 279-285

Publisher:

Elsevier Science Inc, New York

Funding / projects:

DOI: 10.1016/j.jiec.2012.08.012

ISSN: 1226-086X

WoS: 000312764700039

Scopus: 2-s2.0-84870399209

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TY  - JOUR
AU  - Prlainović, Nevena
AU  - Bezbradica, Dejan
AU  - Knežević-Jugović, Zorica
AU  - Stevanović, Sanja
AU  - Avramov-Ivić, Milka
AU  - Uskoković, Petar
AU  - Mijin, Dušan
PY  - 2013
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2477
AB  - In this work lipase from Candida rugosa was adsorbed on unmodified surface of multi walled carbon nanotubes (raw-MWCIsIT). The effects of immobilization time, initial enzyme concentration and buffer ionic strength on enzyme loading and activity of immobilized preparations were tested. High loadings are attained. The immobilized enzyme obtained at lowest initial enzyme concentration and high ionic strength retained 85% of initial enzyme activity. It is assumed that immobilization on hydrophobic surface led to conformational changes that resulted in the adsorption of lipase in active conformation. Immobilized preparations were characterized, with FT-IR spectroscopy, AFM, and cyclic voltammetry.
PB  - Elsevier Science Inc, New York
T2  - Journal of Industrial and Engineering Chemistry
T1  - Adsorption of lipase from Candida rugosa on multi walled carbon nanotubes
EP  - 285
IS  - 1
SP  - 279
VL  - 19
DO  - 10.1016/j.jiec.2012.08.012
ER  -

@article{
author = "Prlainović, Nevena and Bezbradica, Dejan and Knežević-Jugović, Zorica and Stevanović, Sanja and Avramov-Ivić, Milka and Uskoković, Petar and Mijin, Dušan",
year = "2013",
abstract = "In this work lipase from Candida rugosa was adsorbed on unmodified surface of multi walled carbon nanotubes (raw-MWCIsIT). The effects of immobilization time, initial enzyme concentration and buffer ionic strength on enzyme loading and activity of immobilized preparations were tested. High loadings are attained. The immobilized enzyme obtained at lowest initial enzyme concentration and high ionic strength retained 85% of initial enzyme activity. It is assumed that immobilization on hydrophobic surface led to conformational changes that resulted in the adsorption of lipase in active conformation. Immobilized preparations were characterized, with FT-IR spectroscopy, AFM, and cyclic voltammetry.",
publisher = "Elsevier Science Inc, New York",
journal = "Journal of Industrial and Engineering Chemistry",
title = "Adsorption of lipase from Candida rugosa on multi walled carbon nanotubes",
pages = "285-279",
number = "1",
volume = "19",
doi = "10.1016/j.jiec.2012.08.012"
}

Prlainović, N., Bezbradica, D., Knežević-Jugović, Z., Stevanović, S., Avramov-Ivić, M., Uskoković, P.,& Mijin, D.. (2013). Adsorption of lipase from Candida rugosa on multi walled carbon nanotubes. in Journal of Industrial and Engineering Chemistry
Elsevier Science Inc, New York., 19(1), 279-285.
https://doi.org/10.1016/j.jiec.2012.08.012

Prlainović N, Bezbradica D, Knežević-Jugović Z, Stevanović S, Avramov-Ivić M, Uskoković P, Mijin D. Adsorption of lipase from Candida rugosa on multi walled carbon nanotubes. in Journal of Industrial and Engineering Chemistry. 2013;19(1):279-285.
doi:10.1016/j.jiec.2012.08.012 .

Prlainović, Nevena, Bezbradica, Dejan, Knežević-Jugović, Zorica, Stevanović, Sanja, Avramov-Ivić, Milka, Uskoković, Petar, Mijin, Dušan, "Adsorption of lipase from Candida rugosa on multi walled carbon nanotubes" in Journal of Industrial and Engineering Chemistry, 19, no. 1 (2013):279-285,
https://doi.org/10.1016/j.jiec.2012.08.012 . .