Gas exchange is essential for bioreactor cultivation of tissue engineered cartilage
Само за регистроване кориснике
1999
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Tissue engineered cartilage can be grown in vitro if the necessary physical and biochemical factors are present in the tissue culture environment. Cell metabolism and tissue composition were studied for engineered cartilage cultured for 5 weeks using bovine articular chondrocytes, polymer scaffolds (5 mm diameter x 2 mm thick fibrous discs), and rotating bioreactors. Medium pH and concentrations of oxygen, carbon dioxide, glucose, lactate, ammonia, and glycosoaminoglycan (GAG) were varied by altering the exchange rates of gas and medium in the bioreactors. Cell-polymer constructs were assessed with respect to histomorphology, biochemical composition and metabolic activity. Low oxygen tension (similar to 40 mmHg) and low pH (similar to 6.7) were associated with anaerobic cell metabolism (yield of lactate on glucose, Y-L/G, of 2.2 mol/mol) while higher oxygen tension (similar to 80 mmHg) and higher pH (similar to 7.0) were associated with more aerobic cell metabolism (Y-L/G of 1.65-1.79 ...mol/mol). Under conditions of infrequent medium replacement (50% once per week), cells utilized more economical pathways such that glucose consumption and lactate production both decreased, cell metabolism remained relatively aerobic (Y-L/G of 1.67 mol/mol) and the resulting constructs were cartilaginous. More aerobic conditions generally resulted in larger constructs containing higher amounts of cartilaginous tissue components, while anaerobic conditions suppressed chondrogenesis in 3D tissue constructs.
Кључне речи:
bioreactor / tissue engineering / cartilage / chondrocyte metabolism / oxygenИзвор:
Biotechnology and Bioengineering, 1999, 63, 2, 197-205Издавач:
- John Wiley & Sons Inc, New York
DOI: 10.1002/(SICI)1097-0290(19990420)63:2197::AID-BIT83.0.CO;2-2
ISSN: 0006-3592
PubMed: 10099596
WoS: 000078725200008
Scopus: 2-s2.0-0033586733
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Obradović, Bojana AU - Carrier, RL AU - Vunjak-Novaković, Gordana AU - Freed, LE PY - 1999 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/249 AB - Tissue engineered cartilage can be grown in vitro if the necessary physical and biochemical factors are present in the tissue culture environment. Cell metabolism and tissue composition were studied for engineered cartilage cultured for 5 weeks using bovine articular chondrocytes, polymer scaffolds (5 mm diameter x 2 mm thick fibrous discs), and rotating bioreactors. Medium pH and concentrations of oxygen, carbon dioxide, glucose, lactate, ammonia, and glycosoaminoglycan (GAG) were varied by altering the exchange rates of gas and medium in the bioreactors. Cell-polymer constructs were assessed with respect to histomorphology, biochemical composition and metabolic activity. Low oxygen tension (similar to 40 mmHg) and low pH (similar to 6.7) were associated with anaerobic cell metabolism (yield of lactate on glucose, Y-L/G, of 2.2 mol/mol) while higher oxygen tension (similar to 80 mmHg) and higher pH (similar to 7.0) were associated with more aerobic cell metabolism (Y-L/G of 1.65-1.79 mol/mol). Under conditions of infrequent medium replacement (50% once per week), cells utilized more economical pathways such that glucose consumption and lactate production both decreased, cell metabolism remained relatively aerobic (Y-L/G of 1.67 mol/mol) and the resulting constructs were cartilaginous. More aerobic conditions generally resulted in larger constructs containing higher amounts of cartilaginous tissue components, while anaerobic conditions suppressed chondrogenesis in 3D tissue constructs. PB - John Wiley & Sons Inc, New York T2 - Biotechnology and Bioengineering T1 - Gas exchange is essential for bioreactor cultivation of tissue engineered cartilage EP - 205 IS - 2 SP - 197 VL - 63 DO - 10.1002/(SICI)1097-0290(19990420)63:2197::AID-BIT83.0.CO;2-2 ER -
@article{ author = "Obradović, Bojana and Carrier, RL and Vunjak-Novaković, Gordana and Freed, LE", year = "1999", abstract = "Tissue engineered cartilage can be grown in vitro if the necessary physical and biochemical factors are present in the tissue culture environment. Cell metabolism and tissue composition were studied for engineered cartilage cultured for 5 weeks using bovine articular chondrocytes, polymer scaffolds (5 mm diameter x 2 mm thick fibrous discs), and rotating bioreactors. Medium pH and concentrations of oxygen, carbon dioxide, glucose, lactate, ammonia, and glycosoaminoglycan (GAG) were varied by altering the exchange rates of gas and medium in the bioreactors. Cell-polymer constructs were assessed with respect to histomorphology, biochemical composition and metabolic activity. Low oxygen tension (similar to 40 mmHg) and low pH (similar to 6.7) were associated with anaerobic cell metabolism (yield of lactate on glucose, Y-L/G, of 2.2 mol/mol) while higher oxygen tension (similar to 80 mmHg) and higher pH (similar to 7.0) were associated with more aerobic cell metabolism (Y-L/G of 1.65-1.79 mol/mol). Under conditions of infrequent medium replacement (50% once per week), cells utilized more economical pathways such that glucose consumption and lactate production both decreased, cell metabolism remained relatively aerobic (Y-L/G of 1.67 mol/mol) and the resulting constructs were cartilaginous. More aerobic conditions generally resulted in larger constructs containing higher amounts of cartilaginous tissue components, while anaerobic conditions suppressed chondrogenesis in 3D tissue constructs.", publisher = "John Wiley & Sons Inc, New York", journal = "Biotechnology and Bioengineering", title = "Gas exchange is essential for bioreactor cultivation of tissue engineered cartilage", pages = "205-197", number = "2", volume = "63", doi = "10.1002/(SICI)1097-0290(19990420)63:2197::AID-BIT83.0.CO;2-2" }
Obradović, B., Carrier, R., Vunjak-Novaković, G.,& Freed, L.. (1999). Gas exchange is essential for bioreactor cultivation of tissue engineered cartilage. in Biotechnology and Bioengineering John Wiley & Sons Inc, New York., 63(2), 197-205. https://doi.org/10.1002/(SICI)1097-0290(19990420)63:2197::AID-BIT83.0.CO;2-2
Obradović B, Carrier R, Vunjak-Novaković G, Freed L. Gas exchange is essential for bioreactor cultivation of tissue engineered cartilage. in Biotechnology and Bioengineering. 1999;63(2):197-205. doi:10.1002/(SICI)1097-0290(19990420)63:2197::AID-BIT83.0.CO;2-2 .
Obradović, Bojana, Carrier, RL, Vunjak-Novaković, Gordana, Freed, LE, "Gas exchange is essential for bioreactor cultivation of tissue engineered cartilage" in Biotechnology and Bioengineering, 63, no. 2 (1999):197-205, https://doi.org/10.1002/(SICI)1097-0290(19990420)63:2197::AID-BIT83.0.CO;2-2 . .