Bioreactor validation and biocompatibility of Ag/poly(N-vinyl-2-pyrrolidone) hydrogel nanocomposites
Само за регистроване кориснике
2013
Аутори
Jovanović, ŽeljkaRadosavljević, Aleksandra

Kačarević-Popović, Zorica M.

Stojkovska, Jasmina

Perić-Grujić, Aleksandra

Ristić, Mirjana

Matić, Ivana Z.

Juranić, Zorica D.
Obradović, Bojana

Mišković-Stanković, Vesna

Чланак у часопису (Објављена верзија)

Метаподаци
Приказ свих података о документуАпстракт
Silver/poly(N-vinyl-2-pyrrolidone) (Ag/PVP) nanocomposites containing Ag nanoparticles at different concentrations were synthesized using gamma-irradiation. Cytotoxicity of the obtained nanocomposites was determined by MU assay in monolayer cultures of normal human immunocompetent peripheral blood mononuclear cells (PBMC) that were either non-stimulated or stimulated to proliferate by mitogen phytohemagglutinin (PHA), as well as in human cervix adenocarcinoma cell (HeLa) cultures. Silver release kinetics and mechanical properties of nanocomposites were investigated under bioreactor conditions in the simulated body fluid (SBF) at 37 degrees C. The release of silver was monitored under static conditions, and in two types of bioreactors: perfusion bioreactors and a bioreactor with dynamic compression coupled with SBF perfusion simulating in vivo conditions in articular cartilage. Ag/PVP nanocomposites exhibited slight cytotoxic effects against PBMC at the estimated concentration of 0.4 mu... mol dm(-3), with negligible variations observed amongst different cell cultures investigated. Studies of the silver release kinetics indicated internal diffusion as the rate limiting step, determined by statistically comparable results obtained at all investigated conditions. However, silver release rate was slightly higher in the bioreactor with dynamic compression coupled with SBF perfusion as compared to the other two systems indicating the influence of dynamic compression. Modelling of silver release kinetics revealed potentials for optimization of Ag/PVP nanocomposites for particular applications as wound dressings or soft tissue implants.
Кључне речи:
Silver nanoparticles / Poly(N-vinyl-2-pyrrolidone) / In vitro cytotoxicity / Silver release kinetics / Bioreactor conditionsИзвор:
Colloids and Surfaces B-Biointerfaces, 2013, 105, 230-235Издавач:
- Elsevier Science Bv, Amsterdam
Финансирање / пројекти:
- International Atomic Energy Agency, ViennaInternational Atomic Energy Agency [CRP: F23028, 15384]
- Синтеза, развој технологија добијања и примена наноструктурних мултифункционалних материјала дефинисаних својстава (RS-45019)
- Функционални, функционализовани и усавршени нано материјали (RS-45005)
- Модификатори биолошког одговора у физиолошким и патолошким стањима (RS-175011)
DOI: 10.1016/j.colsurfb.2012.12.055
ISSN: 0927-7765
PubMed: 23376750
WoS: 000316589500033
Scopus: 2-s2.0-84873109814
Колекције
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Jovanović, Željka AU - Radosavljević, Aleksandra AU - Kačarević-Popović, Zorica M. AU - Stojkovska, Jasmina AU - Perić-Grujić, Aleksandra AU - Ristić, Mirjana AU - Matić, Ivana Z. AU - Juranić, Zorica D. AU - Obradović, Bojana AU - Mišković-Stanković, Vesna PY - 2013 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2538 AB - Silver/poly(N-vinyl-2-pyrrolidone) (Ag/PVP) nanocomposites containing Ag nanoparticles at different concentrations were synthesized using gamma-irradiation. Cytotoxicity of the obtained nanocomposites was determined by MU assay in monolayer cultures of normal human immunocompetent peripheral blood mononuclear cells (PBMC) that were either non-stimulated or stimulated to proliferate by mitogen phytohemagglutinin (PHA), as well as in human cervix adenocarcinoma cell (HeLa) cultures. Silver release kinetics and mechanical properties of nanocomposites were investigated under bioreactor conditions in the simulated body fluid (SBF) at 37 degrees C. The release of silver was monitored under static conditions, and in two types of bioreactors: perfusion bioreactors and a bioreactor with dynamic compression coupled with SBF perfusion simulating in vivo conditions in articular cartilage. Ag/PVP nanocomposites exhibited slight cytotoxic effects against PBMC at the estimated concentration of 0.4 mu mol dm(-3), with negligible variations observed amongst different cell cultures investigated. Studies of the silver release kinetics indicated internal diffusion as the rate limiting step, determined by statistically comparable results obtained at all investigated conditions. However, silver release rate was slightly higher in the bioreactor with dynamic compression coupled with SBF perfusion as compared to the other two systems indicating the influence of dynamic compression. Modelling of silver release kinetics revealed potentials for optimization of Ag/PVP nanocomposites for particular applications as wound dressings or soft tissue implants. PB - Elsevier Science Bv, Amsterdam T2 - Colloids and Surfaces B-Biointerfaces T1 - Bioreactor validation and biocompatibility of Ag/poly(N-vinyl-2-pyrrolidone) hydrogel nanocomposites EP - 235 SP - 230 VL - 105 DO - 10.1016/j.colsurfb.2012.12.055 ER -
@article{ author = "Jovanović, Željka and Radosavljević, Aleksandra and Kačarević-Popović, Zorica M. and Stojkovska, Jasmina and Perić-Grujić, Aleksandra and Ristić, Mirjana and Matić, Ivana Z. and Juranić, Zorica D. and Obradović, Bojana and Mišković-Stanković, Vesna", year = "2013", abstract = "Silver/poly(N-vinyl-2-pyrrolidone) (Ag/PVP) nanocomposites containing Ag nanoparticles at different concentrations were synthesized using gamma-irradiation. Cytotoxicity of the obtained nanocomposites was determined by MU assay in monolayer cultures of normal human immunocompetent peripheral blood mononuclear cells (PBMC) that were either non-stimulated or stimulated to proliferate by mitogen phytohemagglutinin (PHA), as well as in human cervix adenocarcinoma cell (HeLa) cultures. Silver release kinetics and mechanical properties of nanocomposites were investigated under bioreactor conditions in the simulated body fluid (SBF) at 37 degrees C. The release of silver was monitored under static conditions, and in two types of bioreactors: perfusion bioreactors and a bioreactor with dynamic compression coupled with SBF perfusion simulating in vivo conditions in articular cartilage. Ag/PVP nanocomposites exhibited slight cytotoxic effects against PBMC at the estimated concentration of 0.4 mu mol dm(-3), with negligible variations observed amongst different cell cultures investigated. Studies of the silver release kinetics indicated internal diffusion as the rate limiting step, determined by statistically comparable results obtained at all investigated conditions. However, silver release rate was slightly higher in the bioreactor with dynamic compression coupled with SBF perfusion as compared to the other two systems indicating the influence of dynamic compression. Modelling of silver release kinetics revealed potentials for optimization of Ag/PVP nanocomposites for particular applications as wound dressings or soft tissue implants.", publisher = "Elsevier Science Bv, Amsterdam", journal = "Colloids and Surfaces B-Biointerfaces", title = "Bioreactor validation and biocompatibility of Ag/poly(N-vinyl-2-pyrrolidone) hydrogel nanocomposites", pages = "235-230", volume = "105", doi = "10.1016/j.colsurfb.2012.12.055" }
Jovanović, Ž., Radosavljević, A., Kačarević-Popović, Z. M., Stojkovska, J., Perić-Grujić, A., Ristić, M., Matić, I. Z., Juranić, Z. D., Obradović, B.,& Mišković-Stanković, V.. (2013). Bioreactor validation and biocompatibility of Ag/poly(N-vinyl-2-pyrrolidone) hydrogel nanocomposites. in Colloids and Surfaces B-Biointerfaces Elsevier Science Bv, Amsterdam., 105, 230-235. https://doi.org/10.1016/j.colsurfb.2012.12.055
Jovanović Ž, Radosavljević A, Kačarević-Popović ZM, Stojkovska J, Perić-Grujić A, Ristić M, Matić IZ, Juranić ZD, Obradović B, Mišković-Stanković V. Bioreactor validation and biocompatibility of Ag/poly(N-vinyl-2-pyrrolidone) hydrogel nanocomposites. in Colloids and Surfaces B-Biointerfaces. 2013;105:230-235. doi:10.1016/j.colsurfb.2012.12.055 .
Jovanović, Željka, Radosavljević, Aleksandra, Kačarević-Popović, Zorica M., Stojkovska, Jasmina, Perić-Grujić, Aleksandra, Ristić, Mirjana, Matić, Ivana Z., Juranić, Zorica D., Obradović, Bojana, Mišković-Stanković, Vesna, "Bioreactor validation and biocompatibility of Ag/poly(N-vinyl-2-pyrrolidone) hydrogel nanocomposites" in Colloids and Surfaces B-Biointerfaces, 105 (2013):230-235, https://doi.org/10.1016/j.colsurfb.2012.12.055 . .