Silver/poly(N-vinyl-2-pyrrolidone) (Ag/PVP) nanocomposites containing Ag nanoparticles at different concentrations were synthesized using gamma-irradiation. Cytotoxicity of the obtained nanocomposites was determined by MU assay in monolayer cultures of normal human immunocompetent peripheral blood mononuclear cells (PBMC) that were either non-stimulated or stimulated to proliferate by mitogen phytohemagglutinin (PHA), as well as in human cervix adenocarcinoma cell (HeLa) cultures. Silver release kinetics and mechanical properties of nanocomposites were investigated under bioreactor conditions in the simulated body fluid (SBF) at 37 degrees C. The release of silver was monitored under static conditions, and in two types of bioreactors: perfusion bioreactors and a bioreactor with dynamic compression coupled with SBF perfusion simulating in vivo conditions in articular cartilage. Ag/PVP nanocomposites exhibited slight cytotoxic effects against PBMC at the estimated concentration of 0.4 mu... mol dm(-3), with negligible variations observed amongst different cell cultures investigated. Studies of the silver release kinetics indicated internal diffusion as the rate limiting step, determined by statistically comparable results obtained at all investigated conditions. However, silver release rate was slightly higher in the bioreactor with dynamic compression coupled with SBF perfusion as compared to the other two systems indicating the influence of dynamic compression. Modelling of silver release kinetics revealed potentials for optimization of Ag/PVP nanocomposites for particular applications as wound dressings or soft tissue implants.
TY - JOUR
AU - Jovanović, Željka
AU - Radosavljević, Aleksandra
AU - Kačarević-Popović, Zorica M.
AU - Stojkovska, Jasmina
AU - Perić-Grujić, Aleksandra
AU - Ristić, Mirjana
AU - Matić, Ivana Z.
AU - Juranić, Zorica D.
AU - Obradović, Bojana
AU - Mišković-Stanković, Vesna
PY - 2013
UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2538
AB - Silver/poly(N-vinyl-2-pyrrolidone) (Ag/PVP) nanocomposites containing Ag nanoparticles at different concentrations were synthesized using gamma-irradiation. Cytotoxicity of the obtained nanocomposites was determined by MU assay in monolayer cultures of normal human immunocompetent peripheral blood mononuclear cells (PBMC) that were either non-stimulated or stimulated to proliferate by mitogen phytohemagglutinin (PHA), as well as in human cervix adenocarcinoma cell (HeLa) cultures. Silver release kinetics and mechanical properties of nanocomposites were investigated under bioreactor conditions in the simulated body fluid (SBF) at 37 degrees C. The release of silver was monitored under static conditions, and in two types of bioreactors: perfusion bioreactors and a bioreactor with dynamic compression coupled with SBF perfusion simulating in vivo conditions in articular cartilage. Ag/PVP nanocomposites exhibited slight cytotoxic effects against PBMC at the estimated concentration of 0.4 mu mol dm(-3), with negligible variations observed amongst different cell cultures investigated. Studies of the silver release kinetics indicated internal diffusion as the rate limiting step, determined by statistically comparable results obtained at all investigated conditions. However, silver release rate was slightly higher in the bioreactor with dynamic compression coupled with SBF perfusion as compared to the other two systems indicating the influence of dynamic compression. Modelling of silver release kinetics revealed potentials for optimization of Ag/PVP nanocomposites for particular applications as wound dressings or soft tissue implants.
PB - Elsevier Science Bv, Amsterdam
T2 - Colloids and Surfaces B-Biointerfaces
T1 - Bioreactor validation and biocompatibility of Ag/poly(N-vinyl-2-pyrrolidone) hydrogel nanocomposites
EP - 235
SP - 230
VL - 105
DO - 10.1016/j.colsurfb.2012.12.055
ER -
@article{
author = "Jovanović, Željka and Radosavljević, Aleksandra and Kačarević-Popović, Zorica M. and Stojkovska, Jasmina and Perić-Grujić, Aleksandra and Ristić, Mirjana and Matić, Ivana Z. and Juranić, Zorica D. and Obradović, Bojana and Mišković-Stanković, Vesna",
year = "2013",
abstract = "Silver/poly(N-vinyl-2-pyrrolidone) (Ag/PVP) nanocomposites containing Ag nanoparticles at different concentrations were synthesized using gamma-irradiation. Cytotoxicity of the obtained nanocomposites was determined by MU assay in monolayer cultures of normal human immunocompetent peripheral blood mononuclear cells (PBMC) that were either non-stimulated or stimulated to proliferate by mitogen phytohemagglutinin (PHA), as well as in human cervix adenocarcinoma cell (HeLa) cultures. Silver release kinetics and mechanical properties of nanocomposites were investigated under bioreactor conditions in the simulated body fluid (SBF) at 37 degrees C. The release of silver was monitored under static conditions, and in two types of bioreactors: perfusion bioreactors and a bioreactor with dynamic compression coupled with SBF perfusion simulating in vivo conditions in articular cartilage. Ag/PVP nanocomposites exhibited slight cytotoxic effects against PBMC at the estimated concentration of 0.4 mu mol dm(-3), with negligible variations observed amongst different cell cultures investigated. Studies of the silver release kinetics indicated internal diffusion as the rate limiting step, determined by statistically comparable results obtained at all investigated conditions. However, silver release rate was slightly higher in the bioreactor with dynamic compression coupled with SBF perfusion as compared to the other two systems indicating the influence of dynamic compression. Modelling of silver release kinetics revealed potentials for optimization of Ag/PVP nanocomposites for particular applications as wound dressings or soft tissue implants.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Colloids and Surfaces B-Biointerfaces",
title = "Bioreactor validation and biocompatibility of Ag/poly(N-vinyl-2-pyrrolidone) hydrogel nanocomposites",
pages = "235-230",
volume = "105",
doi = "10.1016/j.colsurfb.2012.12.055"
}
Jovanović, Ž., Radosavljević, A., Kačarević-Popović, Z. M., Stojkovska, J., Perić-Grujić, A., Ristić, M., Matić, I. Z., Juranić, Z. D., Obradović, B.,& Mišković-Stanković, V.. (2013). Bioreactor validation and biocompatibility of Ag/poly(N-vinyl-2-pyrrolidone) hydrogel nanocomposites. in Colloids and Surfaces B-Biointerfaces
Elsevier Science Bv, Amsterdam., 105, 230-235.
https://doi.org/10.1016/j.colsurfb.2012.12.055
Jovanović Ž, Radosavljević A, Kačarević-Popović ZM, Stojkovska J, Perić-Grujić A, Ristić M, Matić IZ, Juranić ZD, Obradović B, Mišković-Stanković V. Bioreactor validation and biocompatibility of Ag/poly(N-vinyl-2-pyrrolidone) hydrogel nanocomposites. in Colloids and Surfaces B-Biointerfaces. 2013;105:230-235.
doi:10.1016/j.colsurfb.2012.12.055 .
Jovanović, Željka, Radosavljević, Aleksandra, Kačarević-Popović, Zorica M., Stojkovska, Jasmina, Perić-Grujić, Aleksandra, Ristić, Mirjana, Matić, Ivana Z., Juranić, Zorica D., Obradović, Bojana, Mišković-Stanković, Vesna, "Bioreactor validation and biocompatibility of Ag/poly(N-vinyl-2-pyrrolidone) hydrogel nanocomposites" in Colloids and Surfaces B-Biointerfaces, 105 (2013):230-235,
https://doi.org/10.1016/j.colsurfb.2012.12.055 . .
