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Cryopreservation of cherry rootstock Gisela 5 using vitrification procedure

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2014
234_2013-HORTSCI.pdf (1.379Mb)
Authors
Ružić, Đurđina
Vujović, Tatjana
Cerović, Radosav
Article (Published version)
Metadata
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Abstract
In vitro-grown shoot tips of Gisela 5 (Prunus cerasus x Prunus canescens) cherry rootstock were tested for regrowth after cryopreservation using vitrification technique. Explants were precultured in the dark at 23 degrees C, in a liquid MS medium with a progressively increasing sucrose concentration (0.3 M for 15 h, then 0.7 M for 5 h), and subsequently loaded in a solution containing 2 M glycerol and 0.4 M sucrose for 20 minutes. Shoot tips were dehydrated at 0 degrees C using either the original PVS2 or modified PVS2 solution (PVS A3 - 22.5% sucrose, 37.5% glycerol, 15% ethylene glycol and 15% DMSO) for 30,40 and 50 minutes. The survival and regrowth of the cryopreserved shoot tips dehydrated with the original PVS2 solution ranged between 36-54% and 8-17%, respectively. However, the dehydration with the PVS A3 solution resulted in considerably higher survival rates (81-92%), as well as higher regrowth rates (39-56%) after cryopreservation. These results prove the feasibility of the P...VS A3-based vitrification technique for a long-term storage of this genotype.

Keywords:
Prunus cerasus x Prunus canescens / liquid nitrogen / vitrification solutions
Source:
Horticultural Science, 2014, 41, 2, 55-63
Publisher:
  • Czech Academy of Agricultural Sciences

ISSN: 0862-867X

PubMed:

WoS: 000338096300002

Scopus: 2-s2.0-84902440320
[ Google Scholar ]
7
5
Handle
https://hdl.handle.net/21.15107/rcub_technorep_2560
URI
http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2560
Collections
  • Radovi istraživača (Inovacioni centar) / Researchers’ publications (Innovation Centre)
Institution/Community
Inovacioni centar
TY  - JOUR
AU  - Ružić, Đurđina
AU  - Vujović, Tatjana
AU  - Cerović, Radosav
PY  - 2014
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2560
AB  - In vitro-grown shoot tips of Gisela 5 (Prunus cerasus x Prunus canescens) cherry rootstock were tested for regrowth after cryopreservation using vitrification technique. Explants were precultured in the dark at 23 degrees C, in a liquid MS medium with a progressively increasing sucrose concentration (0.3 M for 15 h, then 0.7 M for 5 h), and subsequently loaded in a solution containing 2 M glycerol and 0.4 M sucrose for 20 minutes. Shoot tips were dehydrated at 0 degrees C using either the original PVS2 or modified PVS2 solution (PVS A3 - 22.5% sucrose, 37.5% glycerol, 15% ethylene glycol and 15% DMSO) for 30,40 and 50 minutes. The survival and regrowth of the cryopreserved shoot tips dehydrated with the original PVS2 solution ranged between 36-54% and 8-17%, respectively. However, the dehydration with the PVS A3 solution resulted in considerably higher survival rates (81-92%), as well as higher regrowth rates (39-56%) after cryopreservation. These results prove the feasibility of the PVS A3-based vitrification technique for a long-term storage of this genotype.
PB  - Czech Academy of Agricultural Sciences
T2  - Horticultural Science
T1  - Cryopreservation of cherry rootstock Gisela 5 using vitrification procedure
EP  - 63
IS  - 2
SP  - 55
VL  - 41
UR  - https://hdl.handle.net/21.15107/rcub_technorep_2560
ER  - 
@article{
author = "Ružić, Đurđina and Vujović, Tatjana and Cerović, Radosav",
year = "2014",
abstract = "In vitro-grown shoot tips of Gisela 5 (Prunus cerasus x Prunus canescens) cherry rootstock were tested for regrowth after cryopreservation using vitrification technique. Explants were precultured in the dark at 23 degrees C, in a liquid MS medium with a progressively increasing sucrose concentration (0.3 M for 15 h, then 0.7 M for 5 h), and subsequently loaded in a solution containing 2 M glycerol and 0.4 M sucrose for 20 minutes. Shoot tips were dehydrated at 0 degrees C using either the original PVS2 or modified PVS2 solution (PVS A3 - 22.5% sucrose, 37.5% glycerol, 15% ethylene glycol and 15% DMSO) for 30,40 and 50 minutes. The survival and regrowth of the cryopreserved shoot tips dehydrated with the original PVS2 solution ranged between 36-54% and 8-17%, respectively. However, the dehydration with the PVS A3 solution resulted in considerably higher survival rates (81-92%), as well as higher regrowth rates (39-56%) after cryopreservation. These results prove the feasibility of the PVS A3-based vitrification technique for a long-term storage of this genotype.",
publisher = "Czech Academy of Agricultural Sciences",
journal = "Horticultural Science",
title = "Cryopreservation of cherry rootstock Gisela 5 using vitrification procedure",
pages = "63-55",
number = "2",
volume = "41",
url = "https://hdl.handle.net/21.15107/rcub_technorep_2560"
}
Ružić, Đ., Vujović, T.,& Cerović, R.. (2014). Cryopreservation of cherry rootstock Gisela 5 using vitrification procedure. in Horticultural Science
Czech Academy of Agricultural Sciences., 41(2), 55-63.
https://hdl.handle.net/21.15107/rcub_technorep_2560
Ružić Đ, Vujović T, Cerović R. Cryopreservation of cherry rootstock Gisela 5 using vitrification procedure. in Horticultural Science. 2014;41(2):55-63.
https://hdl.handle.net/21.15107/rcub_technorep_2560 .
Ružić, Đurđina, Vujović, Tatjana, Cerović, Radosav, "Cryopreservation of cherry rootstock Gisela 5 using vitrification procedure" in Horticultural Science, 41, no. 2 (2014):55-63,
https://hdl.handle.net/21.15107/rcub_technorep_2560 .

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