Cryopreservation of cherry rootstock Gisela 5 using vitrification procedure
Abstract
In vitro-grown shoot tips of Gisela 5 (Prunus cerasus x Prunus canescens) cherry rootstock were tested for regrowth after cryopreservation using vitrification technique. Explants were precultured in the dark at 23 degrees C, in a liquid MS medium with a progressively increasing sucrose concentration (0.3 M for 15 h, then 0.7 M for 5 h), and subsequently loaded in a solution containing 2 M glycerol and 0.4 M sucrose for 20 minutes. Shoot tips were dehydrated at 0 degrees C using either the original PVS2 or modified PVS2 solution (PVS A3 - 22.5% sucrose, 37.5% glycerol, 15% ethylene glycol and 15% DMSO) for 30,40 and 50 minutes. The survival and regrowth of the cryopreserved shoot tips dehydrated with the original PVS2 solution ranged between 36-54% and 8-17%, respectively. However, the dehydration with the PVS A3 solution resulted in considerably higher survival rates (81-92%), as well as higher regrowth rates (39-56%) after cryopreservation. These results prove the feasibility of the P...VS A3-based vitrification technique for a long-term storage of this genotype.
Keywords:
Prunus cerasus x Prunus canescens / liquid nitrogen / vitrification solutionsSource:
Horticultural Science, 2014, 41, 2, 55-63Publisher:
- Czech Academy of Agricultural Sciences
Institution/Community
Inovacioni centarTY - JOUR AU - Ružić, Đurđina AU - Vujović, Tatjana AU - Cerović, Radosav PY - 2014 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2560 AB - In vitro-grown shoot tips of Gisela 5 (Prunus cerasus x Prunus canescens) cherry rootstock were tested for regrowth after cryopreservation using vitrification technique. Explants were precultured in the dark at 23 degrees C, in a liquid MS medium with a progressively increasing sucrose concentration (0.3 M for 15 h, then 0.7 M for 5 h), and subsequently loaded in a solution containing 2 M glycerol and 0.4 M sucrose for 20 minutes. Shoot tips were dehydrated at 0 degrees C using either the original PVS2 or modified PVS2 solution (PVS A3 - 22.5% sucrose, 37.5% glycerol, 15% ethylene glycol and 15% DMSO) for 30,40 and 50 minutes. The survival and regrowth of the cryopreserved shoot tips dehydrated with the original PVS2 solution ranged between 36-54% and 8-17%, respectively. However, the dehydration with the PVS A3 solution resulted in considerably higher survival rates (81-92%), as well as higher regrowth rates (39-56%) after cryopreservation. These results prove the feasibility of the PVS A3-based vitrification technique for a long-term storage of this genotype. PB - Czech Academy of Agricultural Sciences T2 - Horticultural Science T1 - Cryopreservation of cherry rootstock Gisela 5 using vitrification procedure EP - 63 IS - 2 SP - 55 VL - 41 UR - https://hdl.handle.net/21.15107/rcub_technorep_2560 ER -
@article{ author = "Ružić, Đurđina and Vujović, Tatjana and Cerović, Radosav", year = "2014", abstract = "In vitro-grown shoot tips of Gisela 5 (Prunus cerasus x Prunus canescens) cherry rootstock were tested for regrowth after cryopreservation using vitrification technique. Explants were precultured in the dark at 23 degrees C, in a liquid MS medium with a progressively increasing sucrose concentration (0.3 M for 15 h, then 0.7 M for 5 h), and subsequently loaded in a solution containing 2 M glycerol and 0.4 M sucrose for 20 minutes. Shoot tips were dehydrated at 0 degrees C using either the original PVS2 or modified PVS2 solution (PVS A3 - 22.5% sucrose, 37.5% glycerol, 15% ethylene glycol and 15% DMSO) for 30,40 and 50 minutes. The survival and regrowth of the cryopreserved shoot tips dehydrated with the original PVS2 solution ranged between 36-54% and 8-17%, respectively. However, the dehydration with the PVS A3 solution resulted in considerably higher survival rates (81-92%), as well as higher regrowth rates (39-56%) after cryopreservation. These results prove the feasibility of the PVS A3-based vitrification technique for a long-term storage of this genotype.", publisher = "Czech Academy of Agricultural Sciences", journal = "Horticultural Science", title = "Cryopreservation of cherry rootstock Gisela 5 using vitrification procedure", pages = "63-55", number = "2", volume = "41", url = "https://hdl.handle.net/21.15107/rcub_technorep_2560" }
Ružić, Đ., Vujović, T.,& Cerović, R.. (2014). Cryopreservation of cherry rootstock Gisela 5 using vitrification procedure. in Horticultural Science Czech Academy of Agricultural Sciences., 41(2), 55-63. https://hdl.handle.net/21.15107/rcub_technorep_2560
Ružić Đ, Vujović T, Cerović R. Cryopreservation of cherry rootstock Gisela 5 using vitrification procedure. in Horticultural Science. 2014;41(2):55-63. https://hdl.handle.net/21.15107/rcub_technorep_2560 .
Ružić, Đurđina, Vujović, Tatjana, Cerović, Radosav, "Cryopreservation of cherry rootstock Gisela 5 using vitrification procedure" in Horticultural Science, 41, no. 2 (2014):55-63, https://hdl.handle.net/21.15107/rcub_technorep_2560 .