Приказ основних података о документу

dc.creatorRužić, Đurđina
dc.creatorVujović, Tatjana
dc.creatorCerović, Radosav
dc.date.accessioned2021-03-10T12:17:52Z
dc.date.available2021-03-10T12:17:52Z
dc.date.issued2014
dc.identifier.issn0862-867X
dc.identifier.urihttp://TechnoRep.tmf.bg.ac.rs/handle/123456789/2560
dc.description.abstractIn vitro-grown shoot tips of Gisela 5 (Prunus cerasus x Prunus canescens) cherry rootstock were tested for regrowth after cryopreservation using vitrification technique. Explants were precultured in the dark at 23 degrees C, in a liquid MS medium with a progressively increasing sucrose concentration (0.3 M for 15 h, then 0.7 M for 5 h), and subsequently loaded in a solution containing 2 M glycerol and 0.4 M sucrose for 20 minutes. Shoot tips were dehydrated at 0 degrees C using either the original PVS2 or modified PVS2 solution (PVS A3 - 22.5% sucrose, 37.5% glycerol, 15% ethylene glycol and 15% DMSO) for 30,40 and 50 minutes. The survival and regrowth of the cryopreserved shoot tips dehydrated with the original PVS2 solution ranged between 36-54% and 8-17%, respectively. However, the dehydration with the PVS A3 solution resulted in considerably higher survival rates (81-92%), as well as higher regrowth rates (39-56%) after cryopreservation. These results prove the feasibility of the PVS A3-based vitrification technique for a long-term storage of this genotype.en
dc.publisherCzech Academy of Agricultural Sciences
dc.rightsopenAccess
dc.sourceHorticultural Science
dc.subjectPrunus cerasus x Prunus canescensen
dc.subjectliquid nitrogenen
dc.subjectvitrification solutionsen
dc.titleCryopreservation of cherry rootstock Gisela 5 using vitrification procedureen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage63
dc.citation.issue2
dc.citation.other41(2): 55-63
dc.citation.rankM23
dc.citation.spage55
dc.citation.volume41
dc.identifier.fulltexthttp://TechnoRep.tmf.bg.ac.rs/bitstream/id/11788/234_2013-HORTSCI.pdf
dc.identifier.pmid
dc.identifier.rcubhttps://hdl.handle.net/21.15107/rcub_technorep_2560
dc.identifier.scopus2-s2.0-84902440320
dc.identifier.wos000338096300002
dc.type.versionpublishedVersion


Документи

Thumbnail

Овај документ се појављује у следећим колекцијама

Приказ основних података о документу