In this work, we present a comprehensive approach to evaluation of alginate microbeads with included silver nanoparticles (AgNPs) at the concentration range of 0.3-5 mM for potential biomedical use by combining cytotoxicity, antibacterial activity, and silver release studies. The microbeads were investigated regarding drying and rehydration showing retention of similar to 80-85% of the initial nanoparticles as determined by UV-vis and SEM analyses. Both wet and dry microbeads were shown to release AgNPs and/or ions inducing similar growth delays of Staphylococcus aureus and Escherichia coli at the total released silver concentrations of similar to 10 mu g/ml. On the other hand, these concentrations were highly toxic for bovine chondrocytes in conventional monolayer cultures while nontoxic when cultured in alginate microbeads under biomimetic conditions in 3D perfusion bioreactors. The applied approach outlined directions for further optimization studies demonstrating Ag/alginate microb...eads as potentially attractive components of soft tissue implants as well as antimicrobial wound dressings.
TY - JOUR
AU - Stojkovska, Jasmina
AU - Kostić, Danijela
AU - Jovanović, Zeljka
AU - Vukašinović-Sekulić, Maja
AU - Mišković-Stanković, Vesna
AU - Obradović, Bojana
PY - 2014
UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2650
AB - In this work, we present a comprehensive approach to evaluation of alginate microbeads with included silver nanoparticles (AgNPs) at the concentration range of 0.3-5 mM for potential biomedical use by combining cytotoxicity, antibacterial activity, and silver release studies. The microbeads were investigated regarding drying and rehydration showing retention of similar to 80-85% of the initial nanoparticles as determined by UV-vis and SEM analyses. Both wet and dry microbeads were shown to release AgNPs and/or ions inducing similar growth delays of Staphylococcus aureus and Escherichia coli at the total released silver concentrations of similar to 10 mu g/ml. On the other hand, these concentrations were highly toxic for bovine chondrocytes in conventional monolayer cultures while nontoxic when cultured in alginate microbeads under biomimetic conditions in 3D perfusion bioreactors. The applied approach outlined directions for further optimization studies demonstrating Ag/alginate microbeads as potentially attractive components of soft tissue implants as well as antimicrobial wound dressings.
PB - Elsevier Sci Ltd, Oxford
T2 - Carbohydrate Polymers
T1 - A comprehensive approach to in vitro functional evaluation of Ag/alginate nanocomposite hydrogels
EP - 314
SP - 305
VL - 111
DO - 10.1016/j.carbpol.2014.04.063
ER -
@article{
author = "Stojkovska, Jasmina and Kostić, Danijela and Jovanović, Zeljka and Vukašinović-Sekulić, Maja and Mišković-Stanković, Vesna and Obradović, Bojana",
year = "2014",
abstract = "In this work, we present a comprehensive approach to evaluation of alginate microbeads with included silver nanoparticles (AgNPs) at the concentration range of 0.3-5 mM for potential biomedical use by combining cytotoxicity, antibacterial activity, and silver release studies. The microbeads were investigated regarding drying and rehydration showing retention of similar to 80-85% of the initial nanoparticles as determined by UV-vis and SEM analyses. Both wet and dry microbeads were shown to release AgNPs and/or ions inducing similar growth delays of Staphylococcus aureus and Escherichia coli at the total released silver concentrations of similar to 10 mu g/ml. On the other hand, these concentrations were highly toxic for bovine chondrocytes in conventional monolayer cultures while nontoxic when cultured in alginate microbeads under biomimetic conditions in 3D perfusion bioreactors. The applied approach outlined directions for further optimization studies demonstrating Ag/alginate microbeads as potentially attractive components of soft tissue implants as well as antimicrobial wound dressings.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Carbohydrate Polymers",
title = "A comprehensive approach to in vitro functional evaluation of Ag/alginate nanocomposite hydrogels",
pages = "314-305",
volume = "111",
doi = "10.1016/j.carbpol.2014.04.063"
}
Stojkovska, J., Kostić, D., Jovanović, Z., Vukašinović-Sekulić, M., Mišković-Stanković, V.,& Obradović, B.. (2014). A comprehensive approach to in vitro functional evaluation of Ag/alginate nanocomposite hydrogels. in Carbohydrate Polymers
Elsevier Sci Ltd, Oxford., 111, 305-314.
https://doi.org/10.1016/j.carbpol.2014.04.063
Stojkovska J, Kostić D, Jovanović Z, Vukašinović-Sekulić M, Mišković-Stanković V, Obradović B. A comprehensive approach to in vitro functional evaluation of Ag/alginate nanocomposite hydrogels. in Carbohydrate Polymers. 2014;111:305-314.
doi:10.1016/j.carbpol.2014.04.063 .
Stojkovska, Jasmina, Kostić, Danijela, Jovanović, Zeljka, Vukašinović-Sekulić, Maja, Mišković-Stanković, Vesna, Obradović, Bojana, "A comprehensive approach to in vitro functional evaluation of Ag/alginate nanocomposite hydrogels" in Carbohydrate Polymers, 111 (2014):305-314,
https://doi.org/10.1016/j.carbpol.2014.04.063 . .
