The microbial eukaryote Blastocystis is a prevalent and diverse member of the healthy human gut microbiota
Autori
Scanlan, Pauline D.Stensvold, Christen R.
Rajilić-Stojanović, Mirjana
Heilig, Hans G. H. J.
de Vos, Willem M.
O'Toole, Paul W.
Cotter, Paul D.
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
To date, the majority of research into the human gut microbiota has focused on the bacterial fraction of the community. Inevitably, this has resulted in a poor understanding of the diversity and functionality of other intestinal microorganisms in the human gut. One such nonbacterial member is the microbial eukaryote Blastocystis, which has been implicated in the aetiology of a range of different intestinal and extra-intestinal diseases. However, prevalence data from different studies are conflicting, and crucially, there is limited information on its incidence and diversity in healthy individuals. Here, we survey the prevalence, genetic diversity and temporal stability of Blastocystis in a group of healthy adults (n=105) using a sensitive PCR assay. Blastocystis was present in 56% of our sample set, which is much higher than previously reported from an industrialised county (Ireland). Moreover, a diversity of different subtypes (species) were detected, and Blastocystis was present in a... subset of individuals sampled over a period of time between 6 and 10years, indicating that it is capable of long-term host colonisation. These results show that Blastocystis is a common and diverse member of the healthy gut microbiota, thereby extending our knowledge of the microbial ecology of the healthy human intestine.
Ključne reči:
gut microbiota / Blastocystis / intestinal diseaseIzvor:
FEMS Microbiology Ecology, 2014, 90, 1, 326-330Izdavač:
- Oxford Univ Press, Oxford
Finansiranje / projekti:
- Intra-European Marie Curie Research FellowshipEuropean Union (EU)
DOI: 10.1111/1574-6941.12396
ISSN: 0168-6496
PubMed: 25077936
WoS: 000343809700026
Scopus: 2-s2.0-84907868514
Institucija/grupa
Tehnološko-metalurški fakultetTY - JOUR AU - Scanlan, Pauline D. AU - Stensvold, Christen R. AU - Rajilić-Stojanović, Mirjana AU - Heilig, Hans G. H. J. AU - de Vos, Willem M. AU - O'Toole, Paul W. AU - Cotter, Paul D. PY - 2014 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2741 AB - To date, the majority of research into the human gut microbiota has focused on the bacterial fraction of the community. Inevitably, this has resulted in a poor understanding of the diversity and functionality of other intestinal microorganisms in the human gut. One such nonbacterial member is the microbial eukaryote Blastocystis, which has been implicated in the aetiology of a range of different intestinal and extra-intestinal diseases. However, prevalence data from different studies are conflicting, and crucially, there is limited information on its incidence and diversity in healthy individuals. Here, we survey the prevalence, genetic diversity and temporal stability of Blastocystis in a group of healthy adults (n=105) using a sensitive PCR assay. Blastocystis was present in 56% of our sample set, which is much higher than previously reported from an industrialised county (Ireland). Moreover, a diversity of different subtypes (species) were detected, and Blastocystis was present in a subset of individuals sampled over a period of time between 6 and 10years, indicating that it is capable of long-term host colonisation. These results show that Blastocystis is a common and diverse member of the healthy gut microbiota, thereby extending our knowledge of the microbial ecology of the healthy human intestine. PB - Oxford Univ Press, Oxford T2 - FEMS Microbiology Ecology T1 - The microbial eukaryote Blastocystis is a prevalent and diverse member of the healthy human gut microbiota EP - 330 IS - 1 SP - 326 VL - 90 DO - 10.1111/1574-6941.12396 ER -
@article{ author = "Scanlan, Pauline D. and Stensvold, Christen R. and Rajilić-Stojanović, Mirjana and Heilig, Hans G. H. J. and de Vos, Willem M. and O'Toole, Paul W. and Cotter, Paul D.", year = "2014", abstract = "To date, the majority of research into the human gut microbiota has focused on the bacterial fraction of the community. Inevitably, this has resulted in a poor understanding of the diversity and functionality of other intestinal microorganisms in the human gut. One such nonbacterial member is the microbial eukaryote Blastocystis, which has been implicated in the aetiology of a range of different intestinal and extra-intestinal diseases. However, prevalence data from different studies are conflicting, and crucially, there is limited information on its incidence and diversity in healthy individuals. Here, we survey the prevalence, genetic diversity and temporal stability of Blastocystis in a group of healthy adults (n=105) using a sensitive PCR assay. Blastocystis was present in 56% of our sample set, which is much higher than previously reported from an industrialised county (Ireland). Moreover, a diversity of different subtypes (species) were detected, and Blastocystis was present in a subset of individuals sampled over a period of time between 6 and 10years, indicating that it is capable of long-term host colonisation. These results show that Blastocystis is a common and diverse member of the healthy gut microbiota, thereby extending our knowledge of the microbial ecology of the healthy human intestine.", publisher = "Oxford Univ Press, Oxford", journal = "FEMS Microbiology Ecology", title = "The microbial eukaryote Blastocystis is a prevalent and diverse member of the healthy human gut microbiota", pages = "330-326", number = "1", volume = "90", doi = "10.1111/1574-6941.12396" }
Scanlan, P. D., Stensvold, C. R., Rajilić-Stojanović, M., Heilig, H. G. H. J., de Vos, W. M., O'Toole, P. W.,& Cotter, P. D.. (2014). The microbial eukaryote Blastocystis is a prevalent and diverse member of the healthy human gut microbiota. in FEMS Microbiology Ecology Oxford Univ Press, Oxford., 90(1), 326-330. https://doi.org/10.1111/1574-6941.12396
Scanlan PD, Stensvold CR, Rajilić-Stojanović M, Heilig HGHJ, de Vos WM, O'Toole PW, Cotter PD. The microbial eukaryote Blastocystis is a prevalent and diverse member of the healthy human gut microbiota. in FEMS Microbiology Ecology. 2014;90(1):326-330. doi:10.1111/1574-6941.12396 .
Scanlan, Pauline D., Stensvold, Christen R., Rajilić-Stojanović, Mirjana, Heilig, Hans G. H. J., de Vos, Willem M., O'Toole, Paul W., Cotter, Paul D., "The microbial eukaryote Blastocystis is a prevalent and diverse member of the healthy human gut microbiota" in FEMS Microbiology Ecology, 90, no. 1 (2014):326-330, https://doi.org/10.1111/1574-6941.12396 . .