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Catalyzed Ester Synthesis Using Candida rugosa Lipase Entrapped by Poly(N-isopropylacrylamide-co-itaconic Acid) Hydrogel

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2014
2768.pdf (1.935Mb)
Authors
Milašinović, Nikola
Jakovetić, Sonja
Knežević-Jugović, Zorica
Milosavljević, Nedeljko
Lučić, Marija
Filipović, Jovanka
Kalagasidis Krušić, Melina
Article (Published version)
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Abstract
This study reports the synthesis of polymeric matrices based on N-isopropylacrylamide and itaconic acid and its application for immobilization of lipase from Candida rugosa. The lipase was immobilized by entrapment method. Free and immobilized lipase activities, pH and temperature optima, and storage stability were investigated. The optimum temperature for free and entrapped lipase was found to be 40 and 45 degrees C, while the optimum pH was observed at pH 7 and 8, respectively. Both hydrolytic activity in an aqueous medium and esterolytic activity in an organic medium have been evaluated. Maximum reaction rate (V-max) and Michaelis-Menten constants (K-m) were also determined for immobilized lipase. Storage stability of lipase was increased as a result of immobilization process. Furthermore, the operational stability and reusability of the immobilized lipase in esterification reaction have been studied, and it was observed that after 10 cycles, the residual activity for entrapped lipa...se was as high as 50%, implying that the developed hydrogel and immobilized system could provide a promising solution for the flavor ester synthesis at the industrial scale.

Source:
Scientific World Journal, 2014
Publisher:
  • Hindawi Publishing Corp, New York
Funding / projects:
  • Novel encapsulation and enzyme technologies for designing of new biocatalysts and biologically active compounds targeting enhancement of food quality, safety and competitiveness (RS-46010)
  • Synthesis and characterization of novel functional polymers and polymeric nanocomposites (RS-172062)

DOI: 10.1155/2014/142123

ISSN: 1537-744X

PubMed: 24701136

WoS: 000332230600001

Scopus: 2-s2.0-84896343481
[ Google Scholar ]
10
9
URI
http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2771
Collections
  • Radovi istraživača / Researchers’ publications (TMF)
  • Radovi istraživača (Inovacioni centar) / Researchers’ publications (Innovation Centre)
Institution/Community
Tehnološko-metalurški fakultet
TY  - JOUR
AU  - Milašinović, Nikola
AU  - Jakovetić, Sonja
AU  - Knežević-Jugović, Zorica
AU  - Milosavljević, Nedeljko
AU  - Lučić, Marija
AU  - Filipović, Jovanka
AU  - Kalagasidis Krušić, Melina
PY  - 2014
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2771
AB  - This study reports the synthesis of polymeric matrices based on N-isopropylacrylamide and itaconic acid and its application for immobilization of lipase from Candida rugosa. The lipase was immobilized by entrapment method. Free and immobilized lipase activities, pH and temperature optima, and storage stability were investigated. The optimum temperature for free and entrapped lipase was found to be 40 and 45 degrees C, while the optimum pH was observed at pH 7 and 8, respectively. Both hydrolytic activity in an aqueous medium and esterolytic activity in an organic medium have been evaluated. Maximum reaction rate (V-max) and Michaelis-Menten constants (K-m) were also determined for immobilized lipase. Storage stability of lipase was increased as a result of immobilization process. Furthermore, the operational stability and reusability of the immobilized lipase in esterification reaction have been studied, and it was observed that after 10 cycles, the residual activity for entrapped lipase was as high as 50%, implying that the developed hydrogel and immobilized system could provide a promising solution for the flavor ester synthesis at the industrial scale.
PB  - Hindawi Publishing Corp, New York
T2  - Scientific World Journal
T1  - Catalyzed Ester Synthesis Using Candida rugosa Lipase Entrapped by Poly(N-isopropylacrylamide-co-itaconic Acid) Hydrogel
DO  - 10.1155/2014/142123
ER  - 
@article{
author = "Milašinović, Nikola and Jakovetić, Sonja and Knežević-Jugović, Zorica and Milosavljević, Nedeljko and Lučić, Marija and Filipović, Jovanka and Kalagasidis Krušić, Melina",
year = "2014",
abstract = "This study reports the synthesis of polymeric matrices based on N-isopropylacrylamide and itaconic acid and its application for immobilization of lipase from Candida rugosa. The lipase was immobilized by entrapment method. Free and immobilized lipase activities, pH and temperature optima, and storage stability were investigated. The optimum temperature for free and entrapped lipase was found to be 40 and 45 degrees C, while the optimum pH was observed at pH 7 and 8, respectively. Both hydrolytic activity in an aqueous medium and esterolytic activity in an organic medium have been evaluated. Maximum reaction rate (V-max) and Michaelis-Menten constants (K-m) were also determined for immobilized lipase. Storage stability of lipase was increased as a result of immobilization process. Furthermore, the operational stability and reusability of the immobilized lipase in esterification reaction have been studied, and it was observed that after 10 cycles, the residual activity for entrapped lipase was as high as 50%, implying that the developed hydrogel and immobilized system could provide a promising solution for the flavor ester synthesis at the industrial scale.",
publisher = "Hindawi Publishing Corp, New York",
journal = "Scientific World Journal",
title = "Catalyzed Ester Synthesis Using Candida rugosa Lipase Entrapped by Poly(N-isopropylacrylamide-co-itaconic Acid) Hydrogel",
doi = "10.1155/2014/142123"
}
Milašinović, N., Jakovetić, S., Knežević-Jugović, Z., Milosavljević, N., Lučić, M., Filipović, J.,& Kalagasidis Krušić, M.. (2014). Catalyzed Ester Synthesis Using Candida rugosa Lipase Entrapped by Poly(N-isopropylacrylamide-co-itaconic Acid) Hydrogel. in Scientific World Journal
Hindawi Publishing Corp, New York..
https://doi.org/10.1155/2014/142123
Milašinović N, Jakovetić S, Knežević-Jugović Z, Milosavljević N, Lučić M, Filipović J, Kalagasidis Krušić M. Catalyzed Ester Synthesis Using Candida rugosa Lipase Entrapped by Poly(N-isopropylacrylamide-co-itaconic Acid) Hydrogel. in Scientific World Journal. 2014;.
doi:10.1155/2014/142123 .
Milašinović, Nikola, Jakovetić, Sonja, Knežević-Jugović, Zorica, Milosavljević, Nedeljko, Lučić, Marija, Filipović, Jovanka, Kalagasidis Krušić, Melina, "Catalyzed Ester Synthesis Using Candida rugosa Lipase Entrapped by Poly(N-isopropylacrylamide-co-itaconic Acid) Hydrogel" in Scientific World Journal (2014),
https://doi.org/10.1155/2014/142123 . .

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