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Immunomodulatory effects of carbon nanotubes functionalized with a Toll-like receptor 7 agonist on human dendritic cells
dc.creator | Čolić, Miodrag | |
dc.creator | Dzopalić, Tanja | |
dc.creator | Tomić, Sergej | |
dc.creator | Rajković, Jelena | |
dc.creator | Rudolf, Rebeka | |
dc.creator | Vuković, Goran D. | |
dc.creator | Marinković, Aleksandar | |
dc.creator | Uskoković, Petar | |
dc.date.accessioned | 2021-03-10T12:31:45Z | |
dc.date.available | 2021-03-10T12:31:45Z | |
dc.date.issued | 2014 | |
dc.identifier.issn | 0008-6223 | |
dc.identifier.uri | http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2782 | |
dc.description.abstract | The protocols for the preparation of dendritic cells (DCs) as cancer vaccines based on stimulation of Toll-like receptors (TLRs) are very promising. In this study we covalently attached 7-thia-8-oxoguanosine (7-TOG), a selective endosomal TLR7, to previously oxidized multi-walled carbon nanotubes (o-MWCNTs) (mass ratio 1:5.6, respectively), and tested their ability to activate human monocyte-derived (Mo)DCs. Light, confocal and transmission electron microscopy confirmed efficient phagocytosis of 7-TOG-MWCNTs by MoDCs and their efficient delivery to TLR7(+) endosomes. The biocompatibility studies showed that 7-TOG-MWCNTs, at concentrations lower than 100 mu g/ml, were not cytotoxic for MoDCs. 7-TOG-MV/CNTs (50 mu g/ml) up-regulated CD86 expression, allostimulatory activity, T helper (Th)1- and Th17-polarizing capability of MoDCs. The same concentration of soluble 7-TOG, alone or in combination with control o-MWCNTs, did not have such effects. It can be hypothesized that the efficacy of 7-TOG-MWCNTs in stimulating MoDCs was a consequence of increased intracellular concentration of 7-TOG after internalization of the nano-complexes, because similar Th-polarizing capability could be induced with 10-times higher concentrations of soluble 7-TOG. In conclusion, our results suggest that functionalized MWCNTs may be a promising system for the delivery of drugs to intracellular targets, in order to improve the immunogenic potential of DCs for therapeutic purposes. | en |
dc.publisher | Pergamon-Elsevier Science Ltd, Oxford | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175102/RS// | |
dc.relation | Military Medical Academy, Belgrade [MFVMA/8/12-14] | |
dc.relation | info:eu-repo/grantAgreement/EC/FP7/245916/EU// | |
dc.rights | restrictedAccess | |
dc.source | Carbon | |
dc.title | Immunomodulatory effects of carbon nanotubes functionalized with a Toll-like receptor 7 agonist on human dendritic cells | en |
dc.type | article | |
dc.rights.license | ARR | |
dc.citation.epage | 287 | |
dc.citation.other | 67: 273-287 | |
dc.citation.rank | aM21 | |
dc.citation.spage | 273 | |
dc.citation.volume | 67 | |
dc.identifier.doi | 10.1016/j.carbon.2013.09.090 | |
dc.identifier.scopus | 2-s2.0-84892785218 | |
dc.identifier.wos | 000329379300030 | |
dc.type.version | publishedVersion |