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Preparation of cellulosic fibers with biological activity by immobilization of trypsin on periodate oxidized viscose fibers

Samo za registrovane korisnike
2014
Autori
Nikolić, Tanja
Milanović, Jovana
Kramar, Ana
Petronijević, Živomir
Milenković, Ljubiša
Kostić, Mirjana
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentu
Apstrakt
In this study, a biologically active fibrous material was designed by immobilizing trypsin on viscose fibers. The viscose yarn was first oxidized with sodium periodate to produce aldehyde groups and then employed as a support for subsequent immobilization of trypsin through bovine serum albumin. The oxidation by sodium periodate caused changes in the chemical and physical properties of the modified yarn samples, which were evaluated by determining the aldehyde group content, fineness and tensile strength of yarn. The viscose fibers oxidized under the most severe conditions (0.4 % NaIO4, 360 min) exhibited the maximum amount of introduced aldehyde groups (1.284 mmol/g), but also the highest decrease in tensile strength. The trypsin activity was assayed with N-alpha-benzoyl-DL-arginine p-nitroanilide hydrochloride, whereas the amount of bound trypsin was determined by Bradford method. Trypsin immobilized on oxidized viscose yarn retained 97.3 and 83.8 % of the initial activity over 60 da...ys of storage at 4 and 25 A degrees C, respectively, and remained firmly attached to the carrier. The potential application of obtained bioactive fibers is in the treatment of wounds.

Ključne reči:
Viscose yarn / Periodate oxidation / Tensile strength / BSA / Trypsin / Immobilization / Storage stability
Izvor:
Cellulose, 2014, 21, 3, 1369-1380
Izdavač:
  • Springer, Dordrecht
Finansiranje / projekti:
  • Funkcionalizacija, karakterizacija i primena celuloze i derivata celuloze (RS-172029)

DOI: 10.1007/s10570-014-0171-0

ISSN: 0969-0239

WoS: 000336322800027

Scopus: 2-s2.0-84901195720
[ Google Scholar ]
28
23
URI
http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2835
Kolekcije
  • Radovi istraživača / Researchers’ publications (TMF)
  • Radovi istraživača (Inovacioni centar) / Researchers’ publications (Innovation Centre)
Institucija/grupa
Tehnološko-metalurški fakultet
TY  - JOUR
AU  - Nikolić, Tanja
AU  - Milanović, Jovana
AU  - Kramar, Ana
AU  - Petronijević, Živomir
AU  - Milenković, Ljubiša
AU  - Kostić, Mirjana
PY  - 2014
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2835
AB  - In this study, a biologically active fibrous material was designed by immobilizing trypsin on viscose fibers. The viscose yarn was first oxidized with sodium periodate to produce aldehyde groups and then employed as a support for subsequent immobilization of trypsin through bovine serum albumin. The oxidation by sodium periodate caused changes in the chemical and physical properties of the modified yarn samples, which were evaluated by determining the aldehyde group content, fineness and tensile strength of yarn. The viscose fibers oxidized under the most severe conditions (0.4 % NaIO4, 360 min) exhibited the maximum amount of introduced aldehyde groups (1.284 mmol/g), but also the highest decrease in tensile strength. The trypsin activity was assayed with N-alpha-benzoyl-DL-arginine p-nitroanilide hydrochloride, whereas the amount of bound trypsin was determined by Bradford method. Trypsin immobilized on oxidized viscose yarn retained 97.3 and 83.8 % of the initial activity over 60 days of storage at 4 and 25 A degrees C, respectively, and remained firmly attached to the carrier. The potential application of obtained bioactive fibers is in the treatment of wounds.
PB  - Springer, Dordrecht
T2  - Cellulose
T1  - Preparation of cellulosic fibers with biological activity by immobilization of trypsin on periodate oxidized viscose fibers
EP  - 1380
IS  - 3
SP  - 1369
VL  - 21
DO  - 10.1007/s10570-014-0171-0
ER  - 
@article{
author = "Nikolić, Tanja and Milanović, Jovana and Kramar, Ana and Petronijević, Živomir and Milenković, Ljubiša and Kostić, Mirjana",
year = "2014",
abstract = "In this study, a biologically active fibrous material was designed by immobilizing trypsin on viscose fibers. The viscose yarn was first oxidized with sodium periodate to produce aldehyde groups and then employed as a support for subsequent immobilization of trypsin through bovine serum albumin. The oxidation by sodium periodate caused changes in the chemical and physical properties of the modified yarn samples, which were evaluated by determining the aldehyde group content, fineness and tensile strength of yarn. The viscose fibers oxidized under the most severe conditions (0.4 % NaIO4, 360 min) exhibited the maximum amount of introduced aldehyde groups (1.284 mmol/g), but also the highest decrease in tensile strength. The trypsin activity was assayed with N-alpha-benzoyl-DL-arginine p-nitroanilide hydrochloride, whereas the amount of bound trypsin was determined by Bradford method. Trypsin immobilized on oxidized viscose yarn retained 97.3 and 83.8 % of the initial activity over 60 days of storage at 4 and 25 A degrees C, respectively, and remained firmly attached to the carrier. The potential application of obtained bioactive fibers is in the treatment of wounds.",
publisher = "Springer, Dordrecht",
journal = "Cellulose",
title = "Preparation of cellulosic fibers with biological activity by immobilization of trypsin on periodate oxidized viscose fibers",
pages = "1380-1369",
number = "3",
volume = "21",
doi = "10.1007/s10570-014-0171-0"
}
Nikolić, T., Milanović, J., Kramar, A., Petronijević, Ž., Milenković, L.,& Kostić, M.. (2014). Preparation of cellulosic fibers with biological activity by immobilization of trypsin on periodate oxidized viscose fibers. in Cellulose
Springer, Dordrecht., 21(3), 1369-1380.
https://doi.org/10.1007/s10570-014-0171-0
Nikolić T, Milanović J, Kramar A, Petronijević Ž, Milenković L, Kostić M. Preparation of cellulosic fibers with biological activity by immobilization of trypsin on periodate oxidized viscose fibers. in Cellulose. 2014;21(3):1369-1380.
doi:10.1007/s10570-014-0171-0 .
Nikolić, Tanja, Milanović, Jovana, Kramar, Ana, Petronijević, Živomir, Milenković, Ljubiša, Kostić, Mirjana, "Preparation of cellulosic fibers with biological activity by immobilization of trypsin on periodate oxidized viscose fibers" in Cellulose, 21, no. 3 (2014):1369-1380,
https://doi.org/10.1007/s10570-014-0171-0 . .

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