Determination of S-allelic constitution in some sweet cherry cultivars (Prunus avium L.)

Abstract

The paper presents the results of S-genotyping for sweet cherry cultivars ‘Germersdorfer’, ‘Hedelfinger’ and ‘Junska Rana’ from collections and commercial orchards in the Republic of Serbia and Romania. The use of the polymerase chain reaction (PCR method) with consensus primers for the second intron and allele-specific primers enabled determination of the following S-allelic constitutions in the assessed samples: S3S4 (‘Germersdorfer’ 1), S3S12 (‘Germersdorfer’ 2-8 and ‘Hedelfinger’ 1-2) and S3S5 (‘Hedelfinger’ 3). The results of this investigation confirmed that the methods used for determination of S-genotype are very suitable for reliable and relatively rapid genotyping of sweet cherry cultivars. This is the first report of S-allelic constitution for ‘Junska Rana’ (S3S9), based on which this cultivar has been assigned to incompatibility group XVI.

U radu su predstavljeni rezultati S-genotipizacije sorti trešnje Germersdorfer, Hedelfinger i Junska rana iz kolekcionih i proizvodnih zasada u Republici Srbiji i Rumuniji. Primenom lančane reakcije polimeraze (PCR metoda) sa konsenzus prajmerima za drugi intron i alel-specifičnim prajmerima kod ispitivanih uzoraka su utvrđene alelne konstitucije S3S4 (Germersdorfer 1), S3S12 (Germersdorfer 2-8 i Hedelfinger 1-2) i S3S5 (Hedelfinger 3). Rezultati istraživanja su potvrdili da su korišćene metode determinacije S-genotipa veoma pogodne za pouzdanu i relativno brzu genotipizaciju sorti trešnje. Po prvi put je određena S-alelna konstitucija sorte Junska rana (S3S9), na osnovu koje se ova sorta svrstava u grupu inkompatibilnosti XVI.