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dc.creatorStephansen, Karen
dc.creatorMattebjerg, Maria
dc.creatorWattjes, Jasper
dc.creatorMilivojević, Ana
dc.creatorJessen, Flemming
dc.creatorQvortrup, Klaus
dc.creatorGoycoolea, Francisco M.
dc.creatorChronakis, Ioannis S.
dc.date.accessioned2021-03-10T12:45:33Z
dc.date.available2021-03-10T12:45:33Z
dc.date.issued2015
dc.identifier.issn0141-8130
dc.identifier.urihttp://TechnoRep.tmf.bg.ac.rs/handle/123456789/2996
dc.description.abstractMacrostructures based on natural polymers are subject to large attention, as the application range is wide within the food and pharmaceutical industries. In this study we present nanocomplexes (NCXs) made from electrostatic self-assembly between negatively charged alginate and positively charged fish sarcoplasmic proteins (FSP), prepared by bulk mixing. A concentration screening revealed that there was a range of alginate and FSP concentrations where stable NCXs with similar properties were formed, rather than two exact concentrations. The size of the NCXs was 293 +/- 3 nm, and the zeta potential was -42 +/- 0.3 mV. The NCXs were stable in water, gastric buffer, intestinal buffer and HEPES buffered glycose, and at all pH values from 2 to 9 except pH 3, where they aggregated. When proteolytic enzymes were present in the buffer, the NCXs were degraded. Only at high concentrations the NCXs caused a decreased viability in HeLa and U2OS cell lines. The simple processing procedure and the high stability of the NCXs, makes them excellent candidates for use in the food and pharmaceutical industry.en
dc.publisherElsevier, Amsterdam
dc.relationDanish Strategic Research CouncilDanske Strategiske Forskningsrad (DSF) [DSF -10-93456]
dc.relationDanish Strategic Research Council (FENAMI Project)
dc.rightsrestrictedAccess
dc.sourceInternational Journal of Biological Macromolecules
dc.subjectAlginateen
dc.subjectProteinsen
dc.subjectParticleen
dc.subjectNanocomplexen
dc.subjectDrug deliveryen
dc.titleDesign and characterization of self-assembled fish sarcoplasmic protein-alginate nanocomplexesen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage152
dc.citation.other76: 146-152
dc.citation.rankM21
dc.citation.spage146
dc.citation.volume76
dc.identifier.doi10.1016/j.ijbiomac.2015.02.018
dc.identifier.pmid25709012
dc.identifier.rcubconv_4717
dc.identifier.scopus2-s2.0-84924551687
dc.identifier.wos000353604000019
dc.type.versionpublishedVersion


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