Functional cartilaginous constructs for scientific research and eventual tissue repair were cultivated in bioreactors starting from chondrocytes immobilized on polymeric scaffolds. The scaffolds gradually degraded as the cells regenerated tissue matrix consisting of glycosaminoglycan (GAG) and type II collagen. To facilitate data interpretation and optimize cultivation conditions, a mathematical model was developed which yields the concentrations of oxygen and GAG as functions of time and position in growing tissue. Calculated GAG concentrations were qualitatively and quantitatively consistent with profiles measured via high-resolution image processing of tissue samples cultured at two different oxygen tensions for various periods of time.
TY - JOUR
AU - Obradović, Bojana
AU - Meldon, JH
AU - Freed, LE
AU - Vunjak-Novaković, Gordana
PY - 2000
UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/302
AB - Functional cartilaginous constructs for scientific research and eventual tissue repair were cultivated in bioreactors starting from chondrocytes immobilized on polymeric scaffolds. The scaffolds gradually degraded as the cells regenerated tissue matrix consisting of glycosaminoglycan (GAG) and type II collagen. To facilitate data interpretation and optimize cultivation conditions, a mathematical model was developed which yields the concentrations of oxygen and GAG as functions of time and position in growing tissue. Calculated GAG concentrations were qualitatively and quantitatively consistent with profiles measured via high-resolution image processing of tissue samples cultured at two different oxygen tensions for various periods of time.
PB - Wiley-Blackwell, Malden
T2 - AICHE Journal
T1 - Glycosaminoglycan deposition in engineered cartilage: Experiments and mathematical model
EP - 1871
IS - 9
SP - 1860
VL - 46
DO - 10.1002/aic.690460914
ER -
@article{
author = "Obradović, Bojana and Meldon, JH and Freed, LE and Vunjak-Novaković, Gordana",
year = "2000",
abstract = "Functional cartilaginous constructs for scientific research and eventual tissue repair were cultivated in bioreactors starting from chondrocytes immobilized on polymeric scaffolds. The scaffolds gradually degraded as the cells regenerated tissue matrix consisting of glycosaminoglycan (GAG) and type II collagen. To facilitate data interpretation and optimize cultivation conditions, a mathematical model was developed which yields the concentrations of oxygen and GAG as functions of time and position in growing tissue. Calculated GAG concentrations were qualitatively and quantitatively consistent with profiles measured via high-resolution image processing of tissue samples cultured at two different oxygen tensions for various periods of time.",
publisher = "Wiley-Blackwell, Malden",
journal = "AICHE Journal",
title = "Glycosaminoglycan deposition in engineered cartilage: Experiments and mathematical model",
pages = "1871-1860",
number = "9",
volume = "46",
doi = "10.1002/aic.690460914"
}
Obradović, B., Meldon, J., Freed, L.,& Vunjak-Novaković, G.. (2000). Glycosaminoglycan deposition in engineered cartilage: Experiments and mathematical model. in AICHE Journal
Wiley-Blackwell, Malden., 46(9), 1860-1871.
https://doi.org/10.1002/aic.690460914
Obradović B, Meldon J, Freed L, Vunjak-Novaković G. Glycosaminoglycan deposition in engineered cartilage: Experiments and mathematical model. in AICHE Journal. 2000;46(9):1860-1871.
doi:10.1002/aic.690460914 .
Obradović, Bojana, Meldon, JH, Freed, LE, Vunjak-Novaković, Gordana, "Glycosaminoglycan deposition in engineered cartilage: Experiments and mathematical model" in AICHE Journal, 46, no. 9 (2000):1860-1871,
https://doi.org/10.1002/aic.690460914 . .
