Dissolution of copper mineral phases in biological fluids and the controlled release of copper ions from mineralized alginate hydrogels

2015
Аутори
Bassett, David C.
Madžovska, Ivana
Beckwith, Kai S.
Melo, Thor Bernt

Obradović, Bojana

Sikorski, Pawel

Чланак у часопису (Објављена верзија)

Метаподаци
Приказ свих података о документуАпстракт
Here we investigate the dissolution behaviour of copper minerals contained within biocompatible alginate hydrogels. Copper has a number of biological effects and has most recently been evaluated as an alternative to expensive and controversial growth factors for applications in tissue engineering. Precise control and sustained release of copper ions are important due to a narrow therapeutic window of this potentially toxic ion, and alginate would appear to be a good material of choice for this purpose. We found that aqueously insoluble copper minerals could be precipitated during gelling within or mixed into alginate hydrogels in the form of microbeads prior to gelling to serve as depots of copper. These minerals were found to be soluble in a variety of biological fluids relevant to in vitro and in vivo investigations, and the alginate carrier served as a barrier to diffusion of these ions and therefore offered control over the rate and duration of release (Cu2+ release rates observed ...between 10-750 mu Mol g(-1) h(-1) and duration for up to 32 d). Copper mineral and copper mineralized alginate microbeads were characterized using powder x-ray diffraction, FTIR, thermogravimetric analysis and scanning electron microscopy. Dissolution kinetics were studied based on measurements of copper ion concentrations using colourimetric methods. In addition we characterized the complexes formed between released copper ions and biological fluids by electron paramagnetic spectroscopy which offers an insight into the behaviour of these materials in the body.
Кључне речи:
bioinorganics / controlled release / alginate / biomineralization / biotechnologyИзвор:
Biomedical Materials, 2015, 10, 1Издавач:
- IOP Publishing Ltd, Bristol
Финансирање / пројекти:
- Research Council of NorwayResearch Council of Norway [214607]
- European Cooperation in Science and Technology (COST) framework through the MP1005 NAMABIO action
- Синтеза, развој технологија добијања и примена наноструктурних мултифункционалних материјала дефинисаних својстава (RS-45019)
DOI: 10.1088/1748-6041/10/1/015006
ISSN: 1748-6041
PubMed: 25546880
WoS: 000350975400007
Scopus: 2-s2.0-84924284184
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Bassett, David C. AU - Madžovska, Ivana AU - Beckwith, Kai S. AU - Melo, Thor Bernt AU - Obradović, Bojana AU - Sikorski, Pawel PY - 2015 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3029 AB - Here we investigate the dissolution behaviour of copper minerals contained within biocompatible alginate hydrogels. Copper has a number of biological effects and has most recently been evaluated as an alternative to expensive and controversial growth factors for applications in tissue engineering. Precise control and sustained release of copper ions are important due to a narrow therapeutic window of this potentially toxic ion, and alginate would appear to be a good material of choice for this purpose. We found that aqueously insoluble copper minerals could be precipitated during gelling within or mixed into alginate hydrogels in the form of microbeads prior to gelling to serve as depots of copper. These minerals were found to be soluble in a variety of biological fluids relevant to in vitro and in vivo investigations, and the alginate carrier served as a barrier to diffusion of these ions and therefore offered control over the rate and duration of release (Cu2+ release rates observed between 10-750 mu Mol g(-1) h(-1) and duration for up to 32 d). Copper mineral and copper mineralized alginate microbeads were characterized using powder x-ray diffraction, FTIR, thermogravimetric analysis and scanning electron microscopy. Dissolution kinetics were studied based on measurements of copper ion concentrations using colourimetric methods. In addition we characterized the complexes formed between released copper ions and biological fluids by electron paramagnetic spectroscopy which offers an insight into the behaviour of these materials in the body. PB - IOP Publishing Ltd, Bristol T2 - Biomedical Materials T1 - Dissolution of copper mineral phases in biological fluids and the controlled release of copper ions from mineralized alginate hydrogels IS - 1 VL - 10 DO - 10.1088/1748-6041/10/1/015006 ER -
@article{ author = "Bassett, David C. and Madžovska, Ivana and Beckwith, Kai S. and Melo, Thor Bernt and Obradović, Bojana and Sikorski, Pawel", year = "2015", abstract = "Here we investigate the dissolution behaviour of copper minerals contained within biocompatible alginate hydrogels. Copper has a number of biological effects and has most recently been evaluated as an alternative to expensive and controversial growth factors for applications in tissue engineering. Precise control and sustained release of copper ions are important due to a narrow therapeutic window of this potentially toxic ion, and alginate would appear to be a good material of choice for this purpose. We found that aqueously insoluble copper minerals could be precipitated during gelling within or mixed into alginate hydrogels in the form of microbeads prior to gelling to serve as depots of copper. These minerals were found to be soluble in a variety of biological fluids relevant to in vitro and in vivo investigations, and the alginate carrier served as a barrier to diffusion of these ions and therefore offered control over the rate and duration of release (Cu2+ release rates observed between 10-750 mu Mol g(-1) h(-1) and duration for up to 32 d). Copper mineral and copper mineralized alginate microbeads were characterized using powder x-ray diffraction, FTIR, thermogravimetric analysis and scanning electron microscopy. Dissolution kinetics were studied based on measurements of copper ion concentrations using colourimetric methods. In addition we characterized the complexes formed between released copper ions and biological fluids by electron paramagnetic spectroscopy which offers an insight into the behaviour of these materials in the body.", publisher = "IOP Publishing Ltd, Bristol", journal = "Biomedical Materials", title = "Dissolution of copper mineral phases in biological fluids and the controlled release of copper ions from mineralized alginate hydrogels", number = "1", volume = "10", doi = "10.1088/1748-6041/10/1/015006" }
Bassett, D. C., Madžovska, I., Beckwith, K. S., Melo, T. B., Obradović, B.,& Sikorski, P.. (2015). Dissolution of copper mineral phases in biological fluids and the controlled release of copper ions from mineralized alginate hydrogels. in Biomedical Materials IOP Publishing Ltd, Bristol., 10(1). https://doi.org/10.1088/1748-6041/10/1/015006
Bassett DC, Madžovska I, Beckwith KS, Melo TB, Obradović B, Sikorski P. Dissolution of copper mineral phases in biological fluids and the controlled release of copper ions from mineralized alginate hydrogels. in Biomedical Materials. 2015;10(1). doi:10.1088/1748-6041/10/1/015006 .
Bassett, David C., Madžovska, Ivana, Beckwith, Kai S., Melo, Thor Bernt, Obradović, Bojana, Sikorski, Pawel, "Dissolution of copper mineral phases in biological fluids and the controlled release of copper ions from mineralized alginate hydrogels" in Biomedical Materials, 10, no. 1 (2015), https://doi.org/10.1088/1748-6041/10/1/015006 . .