Tryptophan-functionalized gold nanoparticles for deep UV imaging of microbial cells
Authorized Users Only
2015
Authors
Pajović, Jelena D.
Dojčilović, Radovan

Božanić, Dušan K.

Kascakova, Slavka
Refregiers, Matthieu

Dimitrijević-Branković, Suzana

Vodnik, Vesna

Milosavljević, Aleksandar R.

Piscopiello, Emanuela
Luyt, Adriaan S.

Đoković, Vladimir

Article (Published version)

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Show full item recordAbstract
Biocompatible fluorescent nanostructures were prepared by a functionalization of gold nanoparticles with the amino acid tryptophan. The gold-tryptophan bioconjugates were investigated by TEM and HRTEM and various spectroscopy methods (XPS, FTIR, UV-vis and photoluminescence). It was found that the gold nanoparticles, initially 8 nm in diameter, aggregate in the presence of the amino acid. From the XPS and FTIR spectroscopy results, it was concluded that the tryptophan gold interactions mainly take place via indole and carboxyl groups. Although the indole group is involved in the interaction with the gold surfaces, the tryptophan-gold hybrids showed strong fluorescence due to the presence of multilayers of tryptophan. Deep ultra violet (DUV) imaging performed at the SOLEIL synchrotron showed that It is possible to detect these hybrid nanostructures within Escherichia coli cells.
Keywords:
Gold nanoparticles / Tryptophan / Functionalization / Fluorescence imaging / E. coliSource:
Colloids and Surfaces B-Biointerfaces, 2015, 135, 742-750Publisher:
- Elsevier Science Bv, Amsterdam
Funding / projects:
- [20120810]
- Size-, shape- and structure- dependent properties of nanoparticles and nanocomposites (RS-172056)
- Materials of Reduced Dimensions for Efficient Light Harvesting and Energy conversion (RS-45020)
- Physics of collisions and photo processes in atomic, (bio)molecular and nanosized systems (RS-171020)
DOI: 10.1016/j.colsurfb.2015.08.050
ISSN: 0927-7765
PubMed: 26340364
WoS: 000367410200091
Scopus: 2-s2.0-84940494285
Institution/Community
Tehnološko-metalurški fakultetTY - JOUR AU - Pajović, Jelena D. AU - Dojčilović, Radovan AU - Božanić, Dušan K. AU - Kascakova, Slavka AU - Refregiers, Matthieu AU - Dimitrijević-Branković, Suzana AU - Vodnik, Vesna AU - Milosavljević, Aleksandar R. AU - Piscopiello, Emanuela AU - Luyt, Adriaan S. AU - Đoković, Vladimir PY - 2015 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3102 AB - Biocompatible fluorescent nanostructures were prepared by a functionalization of gold nanoparticles with the amino acid tryptophan. The gold-tryptophan bioconjugates were investigated by TEM and HRTEM and various spectroscopy methods (XPS, FTIR, UV-vis and photoluminescence). It was found that the gold nanoparticles, initially 8 nm in diameter, aggregate in the presence of the amino acid. From the XPS and FTIR spectroscopy results, it was concluded that the tryptophan gold interactions mainly take place via indole and carboxyl groups. Although the indole group is involved in the interaction with the gold surfaces, the tryptophan-gold hybrids showed strong fluorescence due to the presence of multilayers of tryptophan. Deep ultra violet (DUV) imaging performed at the SOLEIL synchrotron showed that It is possible to detect these hybrid nanostructures within Escherichia coli cells. PB - Elsevier Science Bv, Amsterdam T2 - Colloids and Surfaces B-Biointerfaces T1 - Tryptophan-functionalized gold nanoparticles for deep UV imaging of microbial cells EP - 750 SP - 742 VL - 135 DO - 10.1016/j.colsurfb.2015.08.050 ER -
@article{ author = "Pajović, Jelena D. and Dojčilović, Radovan and Božanić, Dušan K. and Kascakova, Slavka and Refregiers, Matthieu and Dimitrijević-Branković, Suzana and Vodnik, Vesna and Milosavljević, Aleksandar R. and Piscopiello, Emanuela and Luyt, Adriaan S. and Đoković, Vladimir", year = "2015", abstract = "Biocompatible fluorescent nanostructures were prepared by a functionalization of gold nanoparticles with the amino acid tryptophan. The gold-tryptophan bioconjugates were investigated by TEM and HRTEM and various spectroscopy methods (XPS, FTIR, UV-vis and photoluminescence). It was found that the gold nanoparticles, initially 8 nm in diameter, aggregate in the presence of the amino acid. From the XPS and FTIR spectroscopy results, it was concluded that the tryptophan gold interactions mainly take place via indole and carboxyl groups. Although the indole group is involved in the interaction with the gold surfaces, the tryptophan-gold hybrids showed strong fluorescence due to the presence of multilayers of tryptophan. Deep ultra violet (DUV) imaging performed at the SOLEIL synchrotron showed that It is possible to detect these hybrid nanostructures within Escherichia coli cells.", publisher = "Elsevier Science Bv, Amsterdam", journal = "Colloids and Surfaces B-Biointerfaces", title = "Tryptophan-functionalized gold nanoparticles for deep UV imaging of microbial cells", pages = "750-742", volume = "135", doi = "10.1016/j.colsurfb.2015.08.050" }
Pajović, J. D., Dojčilović, R., Božanić, D. K., Kascakova, S., Refregiers, M., Dimitrijević-Branković, S., Vodnik, V., Milosavljević, A. R., Piscopiello, E., Luyt, A. S.,& Đoković, V.. (2015). Tryptophan-functionalized gold nanoparticles for deep UV imaging of microbial cells. in Colloids and Surfaces B-Biointerfaces Elsevier Science Bv, Amsterdam., 135, 742-750. https://doi.org/10.1016/j.colsurfb.2015.08.050
Pajović JD, Dojčilović R, Božanić DK, Kascakova S, Refregiers M, Dimitrijević-Branković S, Vodnik V, Milosavljević AR, Piscopiello E, Luyt AS, Đoković V. Tryptophan-functionalized gold nanoparticles for deep UV imaging of microbial cells. in Colloids and Surfaces B-Biointerfaces. 2015;135:742-750. doi:10.1016/j.colsurfb.2015.08.050 .
Pajović, Jelena D., Dojčilović, Radovan, Božanić, Dušan K., Kascakova, Slavka, Refregiers, Matthieu, Dimitrijević-Branković, Suzana, Vodnik, Vesna, Milosavljević, Aleksandar R., Piscopiello, Emanuela, Luyt, Adriaan S., Đoković, Vladimir, "Tryptophan-functionalized gold nanoparticles for deep UV imaging of microbial cells" in Colloids and Surfaces B-Biointerfaces, 135 (2015):742-750, https://doi.org/10.1016/j.colsurfb.2015.08.050 . .