Biocompatible fluorescent nanostructures were prepared by a functionalization of gold nanoparticles with the amino acid tryptophan. The gold-tryptophan bioconjugates were investigated by TEM and HRTEM and various spectroscopy methods (XPS, FTIR, UV-vis and photoluminescence). It was found that the gold nanoparticles, initially 8 nm in diameter, aggregate in the presence of the amino acid. From the XPS and FTIR spectroscopy results, it was concluded that the tryptophan gold interactions mainly take place via indole and carboxyl groups. Although the indole group is involved in the interaction with the gold surfaces, the tryptophan-gold hybrids showed strong fluorescence due to the presence of multilayers of tryptophan. Deep ultra violet (DUV) imaging performed at the SOLEIL synchrotron showed that It is possible to detect these hybrid nanostructures within Escherichia coli cells.
TY - JOUR
AU - Pajović, Jelena D.
AU - Dojčilović, Radovan
AU - Božanić, Dušan K.
AU - Kascakova, Slavka
AU - Refregiers, Matthieu
AU - Dimitrijević-Branković, Suzana
AU - Vodnik, Vesna
AU - Milosavljević, Aleksandar R.
AU - Piscopiello, Emanuela
AU - Luyt, Adriaan S.
AU - Đoković, Vladimir
PY - 2015
UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3102
AB - Biocompatible fluorescent nanostructures were prepared by a functionalization of gold nanoparticles with the amino acid tryptophan. The gold-tryptophan bioconjugates were investigated by TEM and HRTEM and various spectroscopy methods (XPS, FTIR, UV-vis and photoluminescence). It was found that the gold nanoparticles, initially 8 nm in diameter, aggregate in the presence of the amino acid. From the XPS and FTIR spectroscopy results, it was concluded that the tryptophan gold interactions mainly take place via indole and carboxyl groups. Although the indole group is involved in the interaction with the gold surfaces, the tryptophan-gold hybrids showed strong fluorescence due to the presence of multilayers of tryptophan. Deep ultra violet (DUV) imaging performed at the SOLEIL synchrotron showed that It is possible to detect these hybrid nanostructures within Escherichia coli cells.
PB - Elsevier Science Bv, Amsterdam
T2 - Colloids and Surfaces B-Biointerfaces
T1 - Tryptophan-functionalized gold nanoparticles for deep UV imaging of microbial cells
EP - 750
SP - 742
VL - 135
DO - 10.1016/j.colsurfb.2015.08.050
ER -
@article{
author = "Pajović, Jelena D. and Dojčilović, Radovan and Božanić, Dušan K. and Kascakova, Slavka and Refregiers, Matthieu and Dimitrijević-Branković, Suzana and Vodnik, Vesna and Milosavljević, Aleksandar R. and Piscopiello, Emanuela and Luyt, Adriaan S. and Đoković, Vladimir",
year = "2015",
abstract = "Biocompatible fluorescent nanostructures were prepared by a functionalization of gold nanoparticles with the amino acid tryptophan. The gold-tryptophan bioconjugates were investigated by TEM and HRTEM and various spectroscopy methods (XPS, FTIR, UV-vis and photoluminescence). It was found that the gold nanoparticles, initially 8 nm in diameter, aggregate in the presence of the amino acid. From the XPS and FTIR spectroscopy results, it was concluded that the tryptophan gold interactions mainly take place via indole and carboxyl groups. Although the indole group is involved in the interaction with the gold surfaces, the tryptophan-gold hybrids showed strong fluorescence due to the presence of multilayers of tryptophan. Deep ultra violet (DUV) imaging performed at the SOLEIL synchrotron showed that It is possible to detect these hybrid nanostructures within Escherichia coli cells.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Colloids and Surfaces B-Biointerfaces",
title = "Tryptophan-functionalized gold nanoparticles for deep UV imaging of microbial cells",
pages = "750-742",
volume = "135",
doi = "10.1016/j.colsurfb.2015.08.050"
}
Pajović, J. D., Dojčilović, R., Božanić, D. K., Kascakova, S., Refregiers, M., Dimitrijević-Branković, S., Vodnik, V., Milosavljević, A. R., Piscopiello, E., Luyt, A. S.,& Đoković, V.. (2015). Tryptophan-functionalized gold nanoparticles for deep UV imaging of microbial cells. in Colloids and Surfaces B-Biointerfaces
Elsevier Science Bv, Amsterdam., 135, 742-750.
https://doi.org/10.1016/j.colsurfb.2015.08.050
Pajović JD, Dojčilović R, Božanić DK, Kascakova S, Refregiers M, Dimitrijević-Branković S, Vodnik V, Milosavljević AR, Piscopiello E, Luyt AS, Đoković V. Tryptophan-functionalized gold nanoparticles for deep UV imaging of microbial cells. in Colloids and Surfaces B-Biointerfaces. 2015;135:742-750.
doi:10.1016/j.colsurfb.2015.08.050 .
Pajović, Jelena D., Dojčilović, Radovan, Božanić, Dušan K., Kascakova, Slavka, Refregiers, Matthieu, Dimitrijević-Branković, Suzana, Vodnik, Vesna, Milosavljević, Aleksandar R., Piscopiello, Emanuela, Luyt, Adriaan S., Đoković, Vladimir, "Tryptophan-functionalized gold nanoparticles for deep UV imaging of microbial cells" in Colloids and Surfaces B-Biointerfaces, 135 (2015):742-750,
https://doi.org/10.1016/j.colsurfb.2015.08.050 . .
