Interpretative optimization of the isocratic ion chromatographic separation of anions
Abstract
Interpretive retention modeling was utilized to optimize the isocratic ion chromatographic (IC) separation of the nine anions (formate, fluoride, chloride, nitrite, bromide, nitrate, phosphate, sulfate and oxalate). The carbonate-bicarbonate eluent was used and separation was done on a Dionex AS14 ion-exchange column. The influence of combined effects of two mobile phase factors, the total eluent concentration (2-6 mM) and the carbonate/bicaronate ratio from 1:9 to 9:1 (which correspond to pH range 9.35-11.27), on the IC separation was studied. The multiple species analyte/eluent model that takes into account ion-exchange equilibria of the eluent and sample anions was used. In order to estimate the parameters in the model, a non-linear fitting of the retention data, obtained at two-factor three-level experimental design, was applied. To find the optimal conditions in the experimental design, the normalized resolution product as a chromatographic objective function was employed. This cr...iterion includes both the individual peak resolution and the total analysis time. A good agreement between experimental and simulated chromatograms was obtained.
Keywords:
anions / ion chromatography / isocratic / multiple species analyte//eluent / resolution productSource:
Journal of the Serbian Chemical Society, 2016, 81, 6, 661-672Publisher:
- Srpsko hemijsko društvo, Beograd
Funding / projects:
DOI: 10.2298/JSC150927022T
ISSN: 0352-5139
WoS: 000380246500005
Scopus: 2-s2.0-84979058874
Institution/Community
Tehnološko-metalurški fakultetTY - JOUR AU - Todorović, Žaklina AU - Rajaković, Ljubinka V. AU - Onjia, Antonije PY - 2016 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3223 AB - Interpretive retention modeling was utilized to optimize the isocratic ion chromatographic (IC) separation of the nine anions (formate, fluoride, chloride, nitrite, bromide, nitrate, phosphate, sulfate and oxalate). The carbonate-bicarbonate eluent was used and separation was done on a Dionex AS14 ion-exchange column. The influence of combined effects of two mobile phase factors, the total eluent concentration (2-6 mM) and the carbonate/bicaronate ratio from 1:9 to 9:1 (which correspond to pH range 9.35-11.27), on the IC separation was studied. The multiple species analyte/eluent model that takes into account ion-exchange equilibria of the eluent and sample anions was used. In order to estimate the parameters in the model, a non-linear fitting of the retention data, obtained at two-factor three-level experimental design, was applied. To find the optimal conditions in the experimental design, the normalized resolution product as a chromatographic objective function was employed. This criterion includes both the individual peak resolution and the total analysis time. A good agreement between experimental and simulated chromatograms was obtained. PB - Srpsko hemijsko društvo, Beograd T2 - Journal of the Serbian Chemical Society T1 - Interpretative optimization of the isocratic ion chromatographic separation of anions EP - 672 IS - 6 SP - 661 VL - 81 DO - 10.2298/JSC150927022T ER -
@article{ author = "Todorović, Žaklina and Rajaković, Ljubinka V. and Onjia, Antonije", year = "2016", abstract = "Interpretive retention modeling was utilized to optimize the isocratic ion chromatographic (IC) separation of the nine anions (formate, fluoride, chloride, nitrite, bromide, nitrate, phosphate, sulfate and oxalate). The carbonate-bicarbonate eluent was used and separation was done on a Dionex AS14 ion-exchange column. The influence of combined effects of two mobile phase factors, the total eluent concentration (2-6 mM) and the carbonate/bicaronate ratio from 1:9 to 9:1 (which correspond to pH range 9.35-11.27), on the IC separation was studied. The multiple species analyte/eluent model that takes into account ion-exchange equilibria of the eluent and sample anions was used. In order to estimate the parameters in the model, a non-linear fitting of the retention data, obtained at two-factor three-level experimental design, was applied. To find the optimal conditions in the experimental design, the normalized resolution product as a chromatographic objective function was employed. This criterion includes both the individual peak resolution and the total analysis time. A good agreement between experimental and simulated chromatograms was obtained.", publisher = "Srpsko hemijsko društvo, Beograd", journal = "Journal of the Serbian Chemical Society", title = "Interpretative optimization of the isocratic ion chromatographic separation of anions", pages = "672-661", number = "6", volume = "81", doi = "10.2298/JSC150927022T" }
Todorović, Ž., Rajaković, L. V.,& Onjia, A.. (2016). Interpretative optimization of the isocratic ion chromatographic separation of anions. in Journal of the Serbian Chemical Society Srpsko hemijsko društvo, Beograd., 81(6), 661-672. https://doi.org/10.2298/JSC150927022T
Todorović Ž, Rajaković LV, Onjia A. Interpretative optimization of the isocratic ion chromatographic separation of anions. in Journal of the Serbian Chemical Society. 2016;81(6):661-672. doi:10.2298/JSC150927022T .
Todorović, Žaklina, Rajaković, Ljubinka V., Onjia, Antonije, "Interpretative optimization of the isocratic ion chromatographic separation of anions" in Journal of the Serbian Chemical Society, 81, no. 6 (2016):661-672, https://doi.org/10.2298/JSC150927022T . .