Cellulases are industrially important enzymes with a potential to convert cellulose into fermentable sugars. Novel bacterial isolate Paenibacillus sp. CKS1 was tested for cellulase activity and the optimal conditions for carboxymethyl cellulase (CMCase) production were determined. Maximum CMCase activity was obtained in the third passage of the bacterial culture after 3 days of incubation at 30 degrees C. Cellobiose and yeast extract was the optimal source of carbon and nitrogen for induction of CMCase activity. In addition, with initial pH 7 of the medium and 40 ml of working volume in 500 ml culture flasks with shaking at 150 rpm, the maximum CMCase activity in a crude culture supernatant reached value of 0.532 +/- 0.006 U/ml. For crude CMCase, optimal temperature was 50 degrees C and optimal pH 4.8, respectively. HPLC analysis confirmed the bacterium is capable to hydrolise CMC to glucose and other soluble sugars.
Keywords:
Paenibacillus sp. / cellulose / CMCase production / optimal conditions
TY - JOUR
AU - Mihajlovski, Katarina
AU - Davidović, Slađana
AU - Simović, Milica
AU - Radovanović, Neda
AU - Šiler-Marinković, Slavica
AU - Rajilić-Stojanović, Mirjana
AU - Dimitrijević-Branković, Suzana
PY - 2016
UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3229
AB - Cellulases are industrially important enzymes with a potential to convert cellulose into fermentable sugars. Novel bacterial isolate Paenibacillus sp. CKS1 was tested for cellulase activity and the optimal conditions for carboxymethyl cellulase (CMCase) production were determined. Maximum CMCase activity was obtained in the third passage of the bacterial culture after 3 days of incubation at 30 degrees C. Cellobiose and yeast extract was the optimal source of carbon and nitrogen for induction of CMCase activity. In addition, with initial pH 7 of the medium and 40 ml of working volume in 500 ml culture flasks with shaking at 150 rpm, the maximum CMCase activity in a crude culture supernatant reached value of 0.532 +/- 0.006 U/ml. For crude CMCase, optimal temperature was 50 degrees C and optimal pH 4.8, respectively. HPLC analysis confirmed the bacterium is capable to hydrolise CMC to glucose and other soluble sugars.
PB - Savez hemijskih inženjera, Beograd
T2 - Hemijska industrija
T1 - Carboxymethyl cellulase production from a Paenibacillus sp.
EP - 338
IS - 3
SP - 329
VL - 70
DO - 10.2298/HEMIND150222038M
ER -
@article{
author = "Mihajlovski, Katarina and Davidović, Slađana and Simović, Milica and Radovanović, Neda and Šiler-Marinković, Slavica and Rajilić-Stojanović, Mirjana and Dimitrijević-Branković, Suzana",
year = "2016",
abstract = "Cellulases are industrially important enzymes with a potential to convert cellulose into fermentable sugars. Novel bacterial isolate Paenibacillus sp. CKS1 was tested for cellulase activity and the optimal conditions for carboxymethyl cellulase (CMCase) production were determined. Maximum CMCase activity was obtained in the third passage of the bacterial culture after 3 days of incubation at 30 degrees C. Cellobiose and yeast extract was the optimal source of carbon and nitrogen for induction of CMCase activity. In addition, with initial pH 7 of the medium and 40 ml of working volume in 500 ml culture flasks with shaking at 150 rpm, the maximum CMCase activity in a crude culture supernatant reached value of 0.532 +/- 0.006 U/ml. For crude CMCase, optimal temperature was 50 degrees C and optimal pH 4.8, respectively. HPLC analysis confirmed the bacterium is capable to hydrolise CMC to glucose and other soluble sugars.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska industrija",
title = "Carboxymethyl cellulase production from a Paenibacillus sp.",
pages = "338-329",
number = "3",
volume = "70",
doi = "10.2298/HEMIND150222038M"
}
Mihajlovski, K., Davidović, S., Simović, M., Radovanović, N., Šiler-Marinković, S., Rajilić-Stojanović, M.,& Dimitrijević-Branković, S.. (2016). Carboxymethyl cellulase production from a Paenibacillus sp.. in Hemijska industrija
Savez hemijskih inženjera, Beograd., 70(3), 329-338.
https://doi.org/10.2298/HEMIND150222038M
Mihajlovski K, Davidović S, Simović M, Radovanović N, Šiler-Marinković S, Rajilić-Stojanović M, Dimitrijević-Branković S. Carboxymethyl cellulase production from a Paenibacillus sp.. in Hemijska industrija. 2016;70(3):329-338.
doi:10.2298/HEMIND150222038M .
Mihajlovski, Katarina, Davidović, Slađana, Simović, Milica, Radovanović, Neda, Šiler-Marinković, Slavica, Rajilić-Stojanović, Mirjana, Dimitrijević-Branković, Suzana, "Carboxymethyl cellulase production from a Paenibacillus sp." in Hemijska industrija, 70, no. 3 (2016):329-338,
https://doi.org/10.2298/HEMIND150222038M . .
